This product is produced by our partner Aves Labs. Learn more.

Green Fluorescent Protein Antibody (GFP)

Chicken polyclonal antibody

Catalog #: GFP-1010 or GFP-1020 Categories: , Datasheet:

$210.00$435.00

  • SizePrice
Clear
Formulation:
Mixture of Affinity Purified Antibodies and purified IgY
Species Tested:
Mouse
Applications:
WB 1:5000-1:10000IHC 1:2000-1:5000ICC 1:2000-1:5000ELISA
Host Species:
Chicken Polyclonal
Molecular Weight:
27 kDa
Cite This Antibody:
Aves Labs Cat# GFP-1010, RRID:AB_2307313
Antigen/Purification: ExpandCollapse

Chickens were immunized with purified recombinant green fluorescent protein (GFP) emulsified in Freund’s adjuvant. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks. Purified antibody preparations were then mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with thimerosal, and then filter-sterilized.

Synonyms: ExpandCollapse
  • GFP antibody
  • Green fluorescent protein antibody

Storage

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with thimerosal (0.01%,w/v)as an anti-microbial agent.

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Since 50% glycerol is present in the vial, this antibody preparation should remain a liquid at -20°C. For longer storage periods, store at -80°C, but be aware that freezing this preparation may reduce its activity.

Product Specific References

MoChenaTakakoKatobYukioKato (2019), ‘Data on localization of coxsackievirus and adenovirus receptor (CAR) in the embryonic rat brain.’ Science Direct. 10.1016/j.dib.2019.103726.

Beatriz del Blanco, Deisy Guiretti, Romana Tomasoni, María T. Lopez-Cascales, Rafael Muñoz-Viana, Michal Lipinski, Marilyn Scandaglia, Yaiza Coca, Román Olivares, Luis M. Valor, Eloísa Herrera, Angel Barco (2019), ‘CBP and SRF co-regulate dendritic growth and synaptic maturation.’ Cell Death & Differentiation. 10.1038/s41418-019-0285-x.

Marina A. Silveira, Thais T. Zampieri, Isadora C. Furigo, Fernando Abdulkader, Jose Donato Jr., Renata Fraza~o (2019), ‘Acute effects of somatomammotropin hormones on neuronal components of the hypothalamic-pituitary-gonadal axis.’ Brain Research. 10.1016/j.brainres.2019.03.003.

Xuefeng Yuan, Ying Huang, Sarita Shah, Hua Wu, and Laurent Gautron (2016), ‘Levels of Cocaine- and Amphetamine-Regulated Transcript in Vagal Afferents in the Mouse Are Unaltered in Response to Metabolic Challenges.’ eNeuro. 10.1523/ENEURO.0174-16.2016.

Efrain A. Ribeiro, Alexander R. Nectow, Lisa E. Pomeranz, Mats I. Ekstrand, Ja Wook Koo, Eric J. Nestler (2019), ‘Viral labeling of neurons synaptically connected to nucleus accumbens somatostatin interneurons.’ Plos One. 10.1371/journal.pone.0213476.

Mariah L. Hoye, Erica D. Koval, Amy J. Wegener, Theodore S. Hyman, Chengran Yang, David R. O’Brien, Rebecca L. Miller, Tracy Cole, Kathleen M. Schoch, Tao Shen, Tomonori Kunikata, Jean-Philippe Richard, David H. Gutmann, Nicholas J. Maragakis, Holly B. Kordasiewicz, Joseph D. Dougherty and Timothy M. Miller (2017), ‘microRNA profiling reveals marker of motor neuron disease in ALS models .’ The Journal of Neuroscience. 10.1523/JNEUROSCI.3582-16.2017.

Karine Narbonne-Reveau, Cédric Maurange (2019), ‘Developmental regulation of regenerative potential in Drosophila by ecdysone through a bistable loop of ZBTB transcription factors.’ Plos Biology. 10.1371/journal.pbio.3000149.

Masafumi Shimojo, Joseph Madara, Sandra Pankow, Xinran Liu, John Yates III, Thomas C. Su¨dhof, and Anton Maximov (2018), ‘Synaptotagmin-11 mediates a vesicle trafficking pathway that is essential for development and synaptic plasticity.’ Genes & Development. 10.1101/gad.320077.118.

XinHuShangyaoQinXiaoHuangYiminYuanZijianTanYakunGuXueyanChengDanWangXiao-FengLianChengHeZhidaSu (2019), ‘Region-Restrict Astrocytes Exhibit Heterogeneous Susceptibility to Neuronal Reprogramming.’ Stem Cell Reports. 10.1016/j.stemcr.2018.12.017.

Christian Fiebig, Silke Keiner, Birgit Ebert, Iris Schäffner, Ravi Jagasia, D. Chichung Lie, and Ruth Beckervordersandforth (2019), ‘Mitochondrial Dysfunction in Astrocytes Impairs the Generation of Reactive Astrocytes and Enhances Neuronal Cell Death in the Cortex Upon Photothrombotic Lesion.’ Frontiers in Molecular Neuroscience. 10.3389/fnmol.2019.00040.

Zengdi Zhang, Zan Huang, Brianna Ong, Chinmayi Sahu, Hu Zeng, and Hai-Bin Ruan (2019), ‘Bone marrow adipose tissue-derived stem cell factor mediates metabolic regulation of hematopoiesis.’ Haematologica. 10.3324/haematol.2018.205856.

Simone Mayer, Jiadong Chen, Dmitry Velmeshev, Tomasz J. Nowakowski, Alex A. Pollen, Arnold R. Kriegstein (2019), ‘Multimodal Single-Cell Analysis Reveals Physiological Maturation in the Developing Human Neocortex.’ Neuron. 10.1016/j.neuron.2019.01.027.

Amanda L. Zimmerman, Eleni M. Kovatsis, Riana Y. Poszgai, Aniqa Tasnim, Qiyu Zhang, David D. Ginty (2019), ‘Distinct Modes of Presynaptic Inhibition of Cutaneous Afferents and Their Functions in Behavior.’ Neuron. 10.1016/j.neuron.2019.02.002.

Shiquan Chai, Kaixuan Liu, Wanbing Feng, Tiantian Liu, Qian Wang, Rong Zhou, Shiming Chen, Liyan Wang, Guanyu Chen, Tian Ming, Jiandong Zhao, Chuanyong Liu and Bing Xue (2019), ‘Activation of G protein–coupled estrogen receptor (GPER) protects intestine from ischemia/reperfusion injury in mice by protecting the crypt cell proliferation.’ Clinical Science. 10.1042/CS20180919.

Stephan W. Baeuml, Daniela Biechl, and Mario F. Wullimann (2019), ‘Adult islet1 Expression Outlines Ventralized Derivatives Along Zebrafish Neuraxis.’ Frontiers in Neuroanatomy. 10.3389/fnana.2019.00019.

Rafael Krämer Sandra Rode Sebastian Rumpf (2019), ‘Rab11 is required for neurite pruning and developmental membrane protein degradation in Drosophila sensory neurons.’ Developmental Biology. 10.1016/j.ydbio.2019.03.003.

Aaron D. Mickle, Sang Min Won, Kyung Nim Noh, Jangyeol Yoon, Kathleen W. Meacham, Yeguang Xue, Lisa A. McIlvried, Bryan A. Copits, Vijay K. Samineni, Kaitlyn e. Crawford, Do Hoon Kim, Paulome Srivastava, Bong Hoon Kim, Seunghwan Min, Young Shiuan, Yeojeong Yun, Maria A. Payne, Jianpeng Zhang, Hokyung Jang, Yuhang Li, H. Henry Lai, Yonggang Huang, Sung-Il Park, robert W. Gereau IV, John A. Rogers (2019), ‘A wireless closed-loop system for optogenetic peripheral neuromodulation.’ Nature. 10.1038/s41586-018-0823-6.

Kazuhiko Namikawa, Alessandro Dorigo, Marta Zagrebelsky, Giulio Russo, Toni Kirmann, Wieland Fahr, Stefan Du¨bel, Martin Korte, and Reinhard W. Ko¨ster (2019), ‘Modeling neurodegenerative Spinocerebellar Ataxia type 13 in zebrafish using a Purkinje neuron specific tunable co-expression system.’ The Journal of Neuroscience. 10.1523/JNEUROSCI.1862-18.2019.

Katy Ong, Camille Collier and Stephen DiNardo (2019), ‘Multiple feedback mechanisms fine-tune Rho signaling to regulate morphogenetic outcomes.’ The Journal of Cell Science. 10.1242/jcs.224378.

Svetoslav Chakarov, Hwee Ying Lim, Leonard Tan, Sheau Yng Lim, Peter See, Josephine Lum, Xiao-Meng Zhang, Shihui Foo, Satoshi Nakamizo, Kaibo Duan, Wan Ting Kong, Rebecca Gentek, Akhila Balachander, Daniel Carbajo, Camille Bleriot, Benoit Malleret, John Kit Chung Tam, Sonia Baig, Muhammad Shabeer, Sue-Anne Ee Shiow Toh, Andreas Schlitzer, Anis Larbi, Thomas Marichal, Bernard Malissen, Jinmiao Chen, Michael Poidinger, Kenji Kabashima, Marc Bajenoff, Lai Guan Ng, Veronique Angeli, Florent Ginhoux (2019), ‘Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches.’ Science. 10.1126/science.aau0964.

Risa Nomura, Kenichi Kashimada, Hitomi Suzuki, Liang Zhao, Atusmi Hosokawa-Tsuji, Hideo Yagita, Masatoshi Takagi, Yoshiakira Kanai, Josephine Bowles, Peter Koopman, Masami Kanai-Azuma, Tomohiro Morio (2019), ‘Nr5a1 suppression during the fetal period optimizes ovarian development by fine-tuning of Notch signaling.’ Journal of Cell Science. 10.1242/jcs.223768.

Lauren Brilli Skvarca, Hwa In Han, Eugenel B. Espiritu, Maria A. Missinato, Elizabeth R. Rochon, Michael D. McDaniels, Abha S. Bais, Beth L. Roman, Joshua S. Waxman, Simon C. Watkins, Alan J. Davidson, Michael Tsang, Neil A. Hukriede (2019), ‘Enhancing acute kidney injury regeneration by promoting cellular dedifferentiation in zebrafish .’ Disease Models & Mechinisms. 10.1242/dmm.037390.

Daniela P. Orquera, M. Bele´n Tavella, Flavio S. J. de Souza, Sofi´a Nasif, Malcolm J. Low and Marcelo Rubinstein (2019), ‘The homeodomain transcription factor NKX2.1 is essential for the early specification of melanocortin neuron identity and activates Pomc expression in the developing hypothalamus.’ The Journal of Neuroscience. 10.1523/JNEUROSCI.2924-18.2019.

VerónicaMurcia-Belmonte YaizaCoca CeliaVegar Santiago Negueruela Caminode Juan Romero Arturo JoséValiño SalvadorSala RonanDaSilva ArturKania VíctorBorrell Luis M.Martinez LyndaErskine EloísaHerrera (2019), ‘A Retino-retinal Projection Guided by Unc5c Emerged in Species with Retinal Waves.’ Current Biology. 10.1016/j.cub.2019.02.052.

Mallgorzata J. Latallo, Nathan M. Livingston, Bin Wu (2019), ‘Translation imaging of single mRNAs in Established Cell Lines and Primary Cultured Neurons .’ Methods. 10.1016/j.ymeth.2019.03.021.

John P. Russell, Ehsan Mohammadi, Casey O. Ligon, Anthony C. Johnson, Michael D. Gershon, Meenakshi Rao, Yuhong Shen, Chi-Chung Chan, Hilary S. Eidam, Michael P. DeMartino, Mui Cheung, Allen I. Oliff, Sanjay Kumar, Beverley Greenwood-Van Meerveld (2018), ‘Exploring the Potential of RET Kinase Inhibition for Irritable Bowel Syndrome: A Preclinical Investigation in Rodent Models of Colonic Hypersensitivity.’ Journal of Pharmacology and Experimental Therapeutics. 10.1124/jpet.118.252973.

Heuijoon Park, Sonali Lad, Kelsey Boland, Kelly Johnson, Nyssa Readio, Guangchun Jin, Samuel Asfaha, Kelly S. Patterson, Ashok Singh, Xiangdong Yang, Douglas Londono, Anupama Singh, Carol Trempus, Derek Gordon, Timothy C. Wang, Rebecca J. Morris (2018), ‘Bone marrow-derived epithelial cells and hair follicle stem cells contribute to development of chronic cutaneous neoplasms.’ Nature Communications. 10.1038/s41467-018-07688-8.

Warren Pan Jessica M Adams Margaret B Allison Christa Patterson Jonathan N Flak Justin Jones Garth Strohbehn James Trevaskis Christopher J Rhodes David P Olson Martin G Myers, Jr. (2018), ‘Essential Role for Hypothalamic Calcitonin Receptor?Expressing Neurons in the Control of Food Intake by Leptin.’ Endocrinology. 10.1210/en.2017-03259.

Kathrin Koblinger, Céline Jean-Xavier, Sandeep Sharma, Tamás Füzesi, Leanne Young, Shane E. A. Eaton, Charlie Hong Ting Kwok, Jaideep Singh Bains, and Patrick J. Whelan, (2018), ‘Optogenetic Activation of A11 Region Increases Motor Activity.’ Frontiers In Neural Circuits. 10.3389/fncir.2018.00086.

Shiloh R. Lueschow, Jessica Stumphy, Huiyu Gong, Stacy L. Kern, Timothy G. Elgin, Mark A. Underwood, Karen M. Kalanetra, David A. Mills, Melissa H. Wong, David K. Meyerholz, Misty Good, Steven J. McElroy (2018), ‘Loss of murine Paneth cell function alters the immature intestinal microbiome and mimics changes seen in neonatal necrotizing enterocolitis.’ Plos One. 10.1371/journal.pone.0204967.

I-Uen Hsua, Jeremy W. Linsleyb, Jade E. Varineaua, Orie T. Shafera, and John Y. Kuwada (2018), ‘Dstac is required for normal circadian activity rhythms in Drosophila.’ Chronobiology International. 10.1080/07420528.2018.1454937.

Erik Curtis, Joel R. Martin, Brandon Gabel, Nikki Sidhu, Teresa K. Rzesiewicz, Ross Mandeville, Sebastiaan Van Gorp, Marjolein Leerink, Takahiro Tadokoro, Silvia Marsala, Catriona Jamieson, Martin Marsala, and Joseph D. Ciacci (2018), ‘A First-in-Human, Phase I Study of Neural Stem Cell Transplantation for Chronic Spinal Cord Injury.’ Cell Stem Cell. 10.1016/j.stem.2018.05.014.

Benoit Labonté, Yun Ha Jeong, Eric Parise, Orna Issler, Mena Fatma, Olivia Engmann, Kyung-Ah Cho, Rachael Neve, Eric J. Nestler, Ja Wook Koo (2019), ‘Gadd45b mediates depressive-like role through DNA demethylation.’ Nature Scientific Reports. 10.1038/s41598-019-40844-8.

Jichao Sun, Jared Carlson-Stevermer, Utpal Das, Minjie Shen, Marion Delenclos, Amanda M. Snead, So Yeon Koo, Lina Wang, Dianhua Qiao, Jonathan Loi, Andrew J. Petersen, Michael Stockton, Anita Bhattacharyya, Mathew V. Jones, Xinyu Zhao, Pamela J. McLean, Andrew A. Sproul, Krishanu Saha, Subhojit Roy (2019), ‘CRISPR/Cas9 editing of APP C-terminus attenuates ß-cleavage and promotes a-cleavage.’ Nature Communications. 10.1038/s41467-018-07971-8.

Marielle Balzano, Maria De Grandis, Thien-Phong Vu Manh, Michel Aurrand-Lions, Claudine Schiff, Ste ´ phane J.C. Mancini (2019), ‘Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri- sinusoidal Hematopoietic Stem Cell Niche.’ Cell Reports. 10.1016/j.celrep.2019.02.065.

Johanna Finn, Kilian Sottoriva, Kostandin V.Pajcini, Jan K. Kitajewski, Chang Chen, Wei Zhang, Asrar B. Malik, Yuru Liu (2019), ‘Dlk1-Mediated Temporal Regulation of Notch Signaling Is Required for Differentiation of Alveolar Type II to Type I Cells during Repair.’ Cell Reports. 10.1016/j.celrep.2019.02.046.

Brian V. Liena, Mark H. Tuszynskib, Paul Lu (2019), ‘Astrocytes migrate from human neural stem cell grafts and functionally T integrate into the injured rat spinal cord.’ Expirimental Neurology. 10.1016/j.expneurol.2019.01.006.

Kim Kridsada, Jingwen Niu, Zhiping Wang, Parthiv Haldipur, Long Ding, Jian J. Li, Anne G. Lindgren, Eloisa Herrera, Gareth M. Thomas, Victor V. Chizhikov, Kathleen J. Millen,# and Wenqin Luo,# (2019), ‘Roof Plate-Derived Dorsal Midline Radial Glial-like Cells Promote Longitudinal Ascending Axon Growth of Rapidly Adapting Mechanoreceptors during Development.’ Cell Reports. 10.1016/j.celrep.2018.05.025.

Eneritz Rueda-Alaña, Isabel Martínez-Garay, Juan Manuel Encinas, Zoltán Molnár and Fernando García-Moreno, (2018), ‘Dbx1-Derived Pyramidal Neurons Are Generated Locally in the Developing Murine Neocortex.’ Frontiers in Neuroscience. 10.3389/fnins.2018.00792.

ZhenyuChenXudongRenXiangjieXuXiaojieZhangYiHuiZhongliangLiuLeiShiYujiangFangLinMaYangLiuDanielTerheyden-KeighleyLingLiuXiaoqingZhang (2018), ‘Genetic Engineering of Human Embryonic Stem Cells for Precise Cell Fate Tracing during Human Lineage Development.’ Stem Cell Reports. 10.1016/j.stemcr.2018.09.014.

Onkar S. Dhande, Benjamin K. Stafford, Katrin Franke, Rana El-Danaf, Kumiko A. Percival, Ann H. Phan, Peichao Li, Bryan J. Hansen, Phong L. Nguyen, Philipp Berens, W. Rowland Taylor, Edward Callaway, Thomas Euler, and Andrew D. Huberman (2018), ‘Molecular fingerprinting of On-Off direction selective retinal ganglion cells across species and relevance to primate visual circuits.’ The Journal of Neuroscience. 10.1523/JNEUROSCI.1784-18.2018.

Nick A. Guagliardo, Junlan Yao, Eric J. Stipes, Sylvia Cechova, Thu H. Le, Douglas A. Bayliss, David T. Breault, Paula Q. Barrett (2019), ‘Adrenal Tissue-Speci c Deletion of TASK Channels Causes Aldosterone-Driven Angiotensin II-Independent Hypertension.’ Hypertension. 10.1161/HYPERTENSIONAHA.118.11962.

Maria Morena, Robert J. Aukema, Kira D. Leitl, Asim J. Rashid, Haley A. Vecchiarelli, Sheena A. Josselyn and Matthew N. Hill (2019), ‘Upregulation of Anandamide Hydrolysis in the Basolateral Complex of Amygdala Reduces Fear Memory Expression and Indices of Stress and Anxiety.’ The Journal of Neuroscience. 10.1523/JNEUROSCI.2251-18.2018.

Thomas G. McWilliamsIan G. Ganley (2019), ‘Investigating Mitophagy and Mitochondrial Morphology In Vivo Using mito-QC: A Comprehensive Guide.’ Journal of Applied Genetics. 10.1007/978-1-4939-8873-0_41.

Wenyu Ding, Qian Wu, Le Sun, Na Clara Pan and Xiaoqun Wang (2019), ‘Cenpj regulates cilia disassembly and neurogenesis in the developing mouse cortex.’ The Journal of Neuroscience. 10.1523/JNEUROSCI.1849-18.2018.

Taito Matsuda, Takashi Irie, Shutaro Katsurabayashi, Yoshinori Hayashi, Tatsuya Nagai, Nobuhiko Hamazaki, Aliya Mari D. Adefuin, Fumihito Miura, Takashi Ito, Hiroshi Kimura, Katsuhiko Shirahige, Tadayuki Takeda, Katsunori Iwasaki, Takuya Imamura, and Kinichi Nakashima, (2019), ‘Pioneer Factor NeuroD1 Rearranges Transcriptional and Epigenetic Profiles to Execute Microglia-Neuron Conversion.’ Neuron. 10.1016/j.neuron.2018.12.010.

Dong Sun, Xiang-Dong Sun, Lu Zhao, Dae-Hoon Lee, Jin-Xia Hu, Fu-Lei Tang, Jin-Xiu Pan, Lin Mei, Xiao-Juan Zhu, Wen-Cheng Xiong (2018), ‘Neogenin, a regulator of adult hippocampal neurogenesis, prevents depressive-like behavior.’ Cell Death & Disease. 10.1038/s41419-017-0019-2.

Olga A. Mineyeva, Dmitri V. Bezriadnov, Alexander V. Kedrov, Alexander A. Lazutkin, Konstantin V. Anokhin, and Grigori N. Enikolopov (2018), ‘Radiation Induces Distinct Changes in Defined Subpopulations of Neural Stem and Progenitor Cells in the Adult Hippocampus .’ Frontiers in Neuroscience. 10.3389/fnins.2018.01013.

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  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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