Affinity purified from pooled serum. Learn more.

GRIP1 Associated Protein 1 (GRASP1) Antibody

Catalog #: 642-GRASP Categories: , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Expected Reactivity:
Human, Bovine, Canine
Applications:
WB 1:1000 Don't see your application?
Species:
Rabbit
Gene Name:
GRIPAP1
Molecular Weight:
~95 kDa
Cite This Antibody:
PhosphoSolutions Cat# 642-GRASP, RRID:AB_2492135
Antigen/Purification: ExpandCollapse

The antigen is a peptide corresponding to amino acid residues from the C-terminal region of rat GRASP1, conjugated to keyhole limpet hemocyanin (KLH).

The antibody is prepared from rabbit serum by affinity purification via chromatography on an affinity column prepared with the C-terminal peptide used as antigen.

Biological Significance: ExpandCollapse

PDZ domain-containing proteins, such as PSD-95 and GRIP are thought to play key roles in glutamate receptor plasticity. GRIP-associated proteins (GRASPs) that bind to distinct PDZ domains within GRIP also play key roles in regulation of glutamate receptor function. GRASP-1 is a neuronal rasGEF associated with GRIP and AMPA receptors in vivo (Scannevin and Huganir, 2000). Recent work suggests that GRASP-1 may regulate neuronal ras signaling and contribute to the regulation of AMPA receptor distribution by NMDA receptor activity (Ye et al., 2000).

Synonyms: ExpandCollapse

• DKFZp434P0630 antibody
• DXImx47e antibody
• GRAP1_HUMAN antibody
• GRASP 1 antibody
• GRASP-1 antibody
• GRASP1 antibody
• GRIP 1 associated protein 1 antibody
• GRIP associated protein 1 antibody
• GRIP1 associated protein 1 antibody
• GRIP1-associated protein 1 antibody
• GRIPAP 1 antibody
• GRIPAP1 antibody
• KIAA1167 antibody
• MGC126593 antibody
• MGC126595 antibody
• MPMGp800B12492Q3 antibody
• Sfc10 antibody
• TAMALIN antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Scannevin RH, Huganir RL (2000) Postsynaptic organization and regulation of excitatory synapses. Nat Rev Neurosci 1:133-141. PMID: 11252776

Ye B, Liao DZ, Zhang XQ, Zhang PS, Dong HL, Huganir RL (2000) GRASP-1: A neuronal RasGEF associated with the AMPA receptor/GRIP complex. Neuron 26:603-617. PMID: 10896157

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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