Affinity purified from pooled serum. Learn more.

Glycine Receptor Antibody

Catalog #: 700-GLY Categories: , Datasheet:

$365.00

Rabbit polyclonal antibody

Pooled Serum
Size:
200 µg
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Rat, Mouse, Human
Applications:
WB 1:1000IHC 1:1000 Don't see your application?
Species:
Rabbit
Molecular Weight:
~48 kDa
Cite This Antibody:
PhosphoSolutions Cat# 700-GLY, RRID:AB_2492133
Antigen/Purification: ExpandCollapse

The antigen is a peptide from the N-terminus region of the α1-subunit of rat glycine receptor.

The antibody is prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003). The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000). The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins (Beckstead et al., 2001; Mihic et al., 1997). These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001). The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002).

Synonyms: ExpandCollapse

• GLRA1 antibody
• GLRA1_HUMAN antibody
• Glycine receptor 48 kDa subunit antibody
• Glycine receptor alpha 1 antibody
• Glycine receptor strychnine-binding subunit antibody
• Glycine receptor subunit alpha-1 antibody
• Glycine receptor, alpha 1 subunit antibody
• HKPX1 antibody
• STHE antibody

Storage

200 µg antibody vial; lyophilized from 5 mM ammonium bicarbonate. Adequate amount of material to conduct 10-mini Western Blots.

The antibody should be reconstituted in 50 µl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Human


Western Blot: Human
PMID Dilution Publication
10065998 not listed Wick, M.J., et al. 1999 Stable expression of human glycine alpha1 and alpha2 homomeric receptors in mouse L(tk-) cells. J Neurosci Methods 87(1):97-103.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Glycine Receptor Antibody
Western blot of rat spinal cord lysate (SC) showing specific immunolabeling of the ~48 kDa α1- and α2-subunits of the glycine receptor. Immunolabeling was absent from a rat hippocampal lysate (H), as the glycine receptor is not expressed in the hippocampus.

Click here to view our Western blotting and lysate preparation protocols.

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