GluR2 (Ser880) Antibody

Catalog #: p1170-880 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Formulation:
Affinity purified
Species Reactivity:
Rat, Mouse, Human, Chicken, Non-human primate, Zebra fish
Applications:
WB 1:1000
Species:
Rabbit Polyclonal
Gene Name:
GRIA2
Molecular Weight:
~100 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1170-880, RRID:AB_2492131
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho Ser880 of rat GluR2.

The antibody is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

 

Biological Significance: ExpandCollapse

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by ?-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The number of GluR2 subunits in the AMPA receptor complex affects the Ca2+ permeability, rectification and single-channel conductance of AMPA receptors. Ser880 has been identified as the PKC phosphorylation site within the C-terminal region of GluR2 and has been shown to differentially regulate the interaction of the PDZ domain-containing proteins GRIP1 and PICK 1 (Matsuda et al., 1999)

Synonyms: ExpandCollapse

• AMPA 2 antibody
• AMPA selective glutamate receptor 2 antibody
• AMPA-selective glutamate receptor 2 antibody
• AMPA2 antibody
• GluA2 antibody
• GLUR 2 antibody
• GLUR B antibody
• GluR K2 antibody
• GluR-2 antibody
• GluR-B antibody
• GluR-K2 antibody
• GLUR2 antibody
• GLURB antibody
• Glutamate receptor 2 antibody
• Glutamate receptor ionotropic AMPA 2 antibody
• Glutamate receptor ionotropic antibody
• Gria2 antibody
• GRIA2_HUMAN antibody
• HBGR2 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Mao, L.M., He, N., Jin, D.Z. and Wang, J.Q., 2018. Regulation of Phosphorylation of AMPA Glutamate Receptors by Muscarinic M4 Receptors in the Striatum In vivo. Neuroscience, 375, pp.84-93. PMID: 29432883

Lameth, J., Gervais, A., Colin, C., Lévêque, P., Jay, T.M., Edeline, J.M. and Mallat, M., 2017. Acute Neuroinflammation Promotes Cell Responses to 1800 MHz GSM Electromagnetic Fields in the Rat Cerebral Cortex. Neurotoxicity Research, pp.1-16. PMID: 28578480

Singer, B.F., Bubula, N., Przybycien-Szymanska, M.M., Li, D. and Vezina, P., 2016. Stimuli associated with the presence or absence of amphetamine regulate cytoskeletal signaling and behavior. European Neuropsychopharmacology. PMID: 27720500

O’Leary, H., Bernard, P. B., Castano, A. M., & Benke, T. A. (2016). Enhanced long term potentiation and decreased AMPA receptor desensitization in the acute period following a single kainate induced early life seizure. Neurobiology of disease, 87, 134-144. PMID: 26706598

Xue, B., Edwards, M. C., Mao, L. M., Guo, M. L., Jin, D. Z., Fibuch, E. E., & Wang, J. Q. (2014). Rapid and sustained GluA1 S845 phosphorylation in synaptic and extrasynaptic locations in the rat forebrain following amphetamine administration. Neurochemistry international, 64, 48-54. PMID: 24231469

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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GluR2 Ser880 Antibody
Western blot of rat brain lysate showing specific immunolabeling of the ~100 kDa GluR2 protein phosphorylated at Ser880 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

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