Affinity purified from pooled serum. Learn more.

GluR1 (Ser845) Antibody

Catalog #: p1160-845 Categories: , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat, Turtle
Expected Reactivity:
Human, Non-human primate
Applications:
WB 1:1000IHC 1:1000 Don't see your application?
Species:
Rabbit
Gene Name:
GRIA1
Molecular Weight:
~100 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1160-845, RRID:AB_2492128
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser845 of GluR1.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR (Roche et al., 1996; Banke et al., 2000). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory (Lee at al., 2003; Esteban at al., 2003).

Synonyms: ExpandCollapse

• GLUR 1 antibody
• GLUR A antibody
• AMPA 1 antibody
• AMPA selective glutamate receptor 1 antibody
• AMPA-selective glutamate receptor 1 antibody
• GluA1 antibody
• GLUH1 antibody
• GluR K1 antibody
• GluR-1 antibody
• GluR-A antibody
• GluR-K1 antibody
• GLUR1 antibody
• GLURA antibody
• GluRK1 antibody
• Glutamate receptor 1 antibody
• Glutamate receptor ionotropic AMPA 1 antibody
• Glutamate receptor ionotropic antibody
• Glutamate receptor, ionotropic, AMPA 1 antibody
• Gria1 antibody
• GRIA1_HUMAN antibody
• HBGR1 antibody
• MGC133252 antibody
• OTTHUMP00000160643 antibody
• OTTHUMP00000165781 antibody
• OTTHUMP00000224241 antibody
• OTTHUMP00000224242 antibody
• OTTHUMP00000224243 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Mouse | Rat | Turtle


Western Blot: Mouse
PMID Dilution Publication
30831136 1:250 Hikida, T., et al. 2019 Adolescent psychosocial stress enhances sensitization to cocaine exposure in genetically vulnerable mice. Neuroscience research, pii: S0168-0102(19)30021-5.
28613156 1:1000 Musante, V., et al. 2017 Reciprocal regulation of ARPP-16 by PKA and MAST3 kinases provides a cAMP-regulated switch in protein phosphatase 2A inhibition. Elife, 6, p.e24998.
28167675 1:1000 Andrade, E.C., et al. 2017 ARPP-16 is a striatal-enriched inhibitor of protein phosphatase 2A regulated by microtubule-associated serine/threonine kinase 3 (Mast 3 kinase). Journal of Neuroscience, pp.4559-15.
25971971 not listed Tassin, T.C., et al. 2015 Regulation of ERK Kinase by MEK1 Kinase Inhibition in the Brain. Journal of Biological Chemistry, Jun 26;290(26):16319-29.
25649278 not listed Ip, F.C., et al. 2015 Anemoside A3 Enhances Cognition through the Regulation of Synaptic Function and Neuroprotection. Neuropsychopharmacology, 40, 1877–1887.
25525190 not listed Suzuki, K., et al. 2015 In Vivo Pharmacological Characterization of TAK-063, a Potent and Selective Phosphodiesterase 10A Inhibitor with Antipsychotic-Like Activity in Rodents. JPET, 352:471-479.
25173217 1:1000 Mango, D., et al. 2014 Adenosine A1 receptor stimulation reduces D1 receptor-mediated GABAergic transmission from striato-nigral terminals and attenuates l-DOPA-induced dyskinesia in dopamine-denervated mice. Experimental neurology, 261, pp.733-743.
25136890 1:1000 Squillace, M., et al. 2014 Dysfunctional dopaminergic neurotransmission in asocial BTBR mice. Translational psychiatry, 4(8), p.e427.
25115542 not listed Wang, J., et al. 2014 Fluoxetine Improves Behavioral Performance by Suppressing the Production of Soluble β-Amyloid in APP/PS1 Mice. Current Alzheimer Research, 11(7), 672-680.
24920629 not listed Meyer, D.A., et al. 2014 Ischemic stroke injury is mediated by aberrant Cdk5. The Journal of Neuroscience, 34(24), 8259-8267.
24907515 not listed Mao, L.M., et al. 2014 Propofol selectively alters GluA1 AMPA receptor phosphorylation in the hippocampus but not prefrontal cortex in young and aged mice. European journal of pharmacology, 738, 237-244.
23303927 not listed Shen, Y., et al. 2013 Melanocortin-4 receptor regulates hippocampal synaptic plasticity through a protein kinase A-dependent mechanism. J Neurosci. 33(2):464-72.
22753408 1:1000 Santini, E., et al. 2012 Dopamine- and cAMP-regulated phosphoprotein of 32-kDa (DARPP-32)-dependent activation of extracellular signal-regulated kinase (ERK) and mammalian target of rapamycin complex 1 (mTORC1) signaling in experimental parkinsonism. J Biol Chem. 287(33):27806-12.
21946266 not listed Errico, F., et al. 2011 Higher free D-aspartate and N-methyl-D-aspartate levels prevent striatal depotentiation and anticipate L-DOPA-induced dyskinesia. Experimental neurology, 232(2), pp.240-250.
20720111 1:1000 Napolitano, F., et al. 2010 Role of aberrant striatal dopamine D1 receptor/cAMP/protein kinase A/DARPP32 signaling in the paradoxical calming effect of amphetamine. Journal of Neuroscience, 30(33), pp.11043-11056.
20554878 1:500 Sears, R.M., et al. 2010 Regulation of Nucleus Accumbens Activity by the Hypothalamic Neuropeptide Melanin-Concentrating Hormone. J. Neurosci., 30: 8263-8273.
19666609 not listed Rebholz, H., et al. 2009 CK2 negatively regulates G s signaling. PNAS, 106: 14096-14101.
19622833 1:750 Santini, E., et al. 2009 Inhibition of mTOR Signaling in Parkinson’s Disease Prevents L-DOPA–Induced Dyskinesia. Sci. Signal., Jul 2009; 2: ra36.
19017804 not listed Meyer, D.A., et al. 2008 Striatal dysregulation of Cdk5 alters locomotor responses to cocaine, motor learning, and dendritic morphology. PNAS, 105: 18561 – 18566.
18923023 1:250 Nishi, A., et al. 2008 Distinct Roles of PDE4 and PDE10A in the Regulation of cAMP/PKA Signaling in the Striatum. J. Neurosci., 28: 10460– 10471.
18417701 1:500 Sun, X., et al. 2008 Acute and chronic dopamine receptor stimulation modulates AMPA receptor trafficking in nucleus accumbens neurons cocultured with prefrontal cortex neurons. J Neurosci. Apr 16;28(16):4216-30.
17923095 not listed Hu,H., et al. 2007 Emotion enhances learning via norepinephrine regulation of AMPA-receptor trafficking. Cell, 131(1), pp.160-173.


Western Blot: Rat
PMID Dilution Publication
29432883 not listed Mao, L.M., et al. 2018 Regulation of Phosphorylation of AMPA Glutamate Receptors by Muscarinic M4 Receptors in the Striatum In vivo. Neuroscience, 375, pp.84-93.
29417763 not listed Suzuki, K., et al. 2018 Combined treatment with a selective PDE10A inhibitor TAK‐063 and either haloperidol or olanzapine at subeffective doses produces potent antipsychotic‐like effects without affecting plasma prolactin levels and cataleptic responses in rodents. Pharmacology Research & Perspectives, 6(1).
29242119 not listed Xue, B., et al. 2017 Pharmacological modulation of AMPA receptor phosphorylation by dopamine and muscarinic receptor agents in the rat medial prefrontal cortex. European journal of pharmacology. 2017 Dec 11;820:45-52.
28757258 1:1000 Tronci, E., et al. 2017 BDNF over-expression induces striatal serotonin fiber sprouting and increases the susceptibility to l-DOPA-induced dyskinesia in 6-OHDA-lesioned rats. Experimental Neurology Nov;297:73-8.
28578480 not listed Lameth, J., et al. 2017 Acute Neuroinflammation Promotes Cell Responses to 1800 MHz GSM Electromagnetic Fields in the Rat Cerebral Cortex. Neurotoxicity Research, pp.1-16.
28167675 1:1000 Andrade, E.C., et al. 2017 ARPP-16 is a striatal-enriched inhibitor of protein phosphatase 2A regulated by microtubule-associated serine/threonine kinase 3 (Mast 3 kinase). Journal of Neuroscience, pp.4559-15.
27844053 1:5000 Hollis, F., et al. 2016 Involvement of CRFR1 in the basolateral amygdala in the immediate fear extinction deficit. eNeuro, 3(5).
27338925 1:1000 Xue, B., et al. 2016 Amphetamine elevates phosphorylation of eukaryotic initiation factor 2α (eIF2α) in the rat forebrain via activating dopamine D1 and D2 receptors. Brain research, 1646, pp.459-466.
27060412 not listed Xue, B., et al. 2017 Integrated regulation of AMPA glutamate receptor phosphorylation in the striatum by dopamine and acetylcholine. Neuropharmacology, 112, pp.57-65.
26706598 not listed O’Leary, H., et al. 2016 Enhanced long term potentiation and decreased AMPA receptor desensitization in the acute period following a single kainate induced early life seizure. Neurobiology of disease, 87, 134-144.
25689263 1:1000 Mao, L.M., et al. 2015 Dynamic increases in AMPA receptor phosphorylation in the rat hippocampus in response to amphetamine. Journal of neurochemistry. Jun;133(6):795-805.
25525190 not listed Suzuki, K., et al. 2015 In Vivo Pharmacological Characterization of TAK-063, a Potent and Selective Phosphodiesterase 10A Inhibitor with Antipsychotic-Like Activity in Rodents. JPET, 352:471-479.
24920629 not listed Meyer, D.A., et al. 2014 Ischemic stroke injury is mediated by aberrant Cdk5. The Journal of Neuroscience, 34(24), 8259-8267.
24780380 1:500 Bernard, P.B., et al. 2014 Necessary, but not sufficient: insights into the mechanisms of mGluR mediated long-term depression from a rat model of early life seizures. Neuropharmacology, 84, 1-12.
24231469 1:1000 Xue, B., et al. 2014 Rapid and sustained GluA1 S845 phosphorylation in synaptic and extrasynaptic locations in the rat forebrain following amphetamine administration. Neurochemistry international, 64, 48-54.
23303927 not listed Shen, Y., et al. 2013 Melanocortin-4 receptor regulates hippocampal synaptic plasticity through a protein kinase A-dependent mechanism. J Neurosci. 33(2):464-72.
20554878 1:500 Sears, R.M., et al. 2010 Regulation of Nucleus Accumbens Activity by the Hypothalamic Neuropeptide Melanin-Concentrating Hormone. J. Neurosci., 30: 8263 – 8273.
18819923 1:1000 Davies, K.D., et al. 2008 Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not -Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Internalization. J. Biol. Chem., 283: 33138 – 33146.
18417701 1:500 Sun, X., et al. 2008 Acute and chronic dopamine receptor stimulation modulates AMPA receptor trafficking in nucleus accumbens neurons cocultured with prefrontal cortex neurons. J Neurosci. Apr 16;28(16):4216-30.


Western Blot: Turtle
PMID Dilution Publication
19261706 not listed Zheng, Z., et al. 2009 PKA Has a Critical Role in Synaptic Delivery of GluR1- and GluR4- Containing AMPARs During Initial Stages of Acquisition of In Vitro Classical Conditioning. J Neurophysiol, 101: 2539 – 2549.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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