Affinity purified from pooled serum. Learn more.

GluR1 (Ser831) Antibody

Catalog #: p1160-831 Categories: , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Rat
Expected Reactivity:
Chicken, Canine
Applications:
WB 1:1000 Don't see your application?
Species:
Rabbit
Gene Name:
GRIA1
Molecular Weight:
~100 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1160-831, RRID:AB_2492127
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser831 of GluR1.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is potentiated by phosphorylation at Ser831 which has been shown to be mediated by either PKC or CaM kinase II (McGlade-McCulloh et al., 1993; Mammen et al., 1999; Roche et al., 1996). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory (Soderling and Derkach, 2000).

Synonyms: ExpandCollapse

• GLUR 1 antibody
• GLUR A antibody
• AMPA 1 antibody
• AMPA selective glutamate receptor 1 antibody
• AMPA-selective glutamate receptor 1 antibody
• GluA1 antibody
• GLUH1 antibody
• GluR K1 antibody
• GluR-1 antibody
• GluR-A antibody
• GluR-K1 antibody
• GLUR1 antibody
• GLURA antibody
• GluRK1 antibody
• Glutamate receptor 1 antibody
• Glutamate receptor ionotropic AMPA 1 antibody
• Glutamate receptor ionotropic antibody
• Glutamate receptor, ionotropic, AMPA 1 antibody
• Gria1 antibody
• GRIA1_HUMAN antibody
• HBGR1 antibody
• MGC133252 antibody
• OTTHUMP00000160643 antibody
• OTTHUMP00000165781 antibody
• OTTHUMP00000224241 antibody
• OTTHUMP00000224242 antibody
• OTTHUMP00000224243 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Human | Mouse | Rat


Western Blot: Human
PMID Dilution Publication
22427672 not listed Jakan-Sakrikar, N., et al. 2012 Substrate-selective and calcium-independent activation of CaMKII by α-actinin. Journal of Biological Chemistry, 287(19), pp.15275-15283.
21610080 not listed Jiao, Y., et al. 2011 Characterization of a Central Ca2+/Calmodulin-dependent Protein Kinase IIα/β Binding Domain in Densin That Selectively Modulates Glutamate Receptor Subunit Phosphorylation. J. Biol. Chem. 286: 24806 – 24818.


Western Blot: Mouse
PMID Dilution Publication
26255006 1:500 Pasek, J.G., et al. 2015 Differential CaMKII regulation by voltage-gated calcium channels in the striatum. Molecular and Cellular Neuroscience, 68, 234-243.
24907515 not listed Mao, L.M., et al. 2014 Propofol selectively alters GluA1 AMPA receptor phosphorylation in the hippocampus but not prefrontal cortex in young and aged mice. European journal of pharmacology, 738, 237-244.
20554878 1:500 Sears, R.M., et al. 2010 Regulation of Nucleus Accumbens Activity by the Hypothalamic Neuropeptide Melanin-Concentrating Hormone. J. Neurosci., 30: 8263-8273.
17923095 not listed Hu,H., et al. 2007 Emotion enhances learning via norepinephrine regulation of AMPA-receptor trafficking. Cell, 131(1), pp.160-173.


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Western Blot: Rat
PMID Dilution Publication
29432883 not listed Mao, L.M., et al. 2018 Regulation of Phosphorylation of AMPA Glutamate Receptors by Muscarinic M4 Receptors in the Striatum In vivo. Neuroscience, 375, pp.84-93.
29242119 not listed Xue, B., et al. 2017 Pharmacological modulation of AMPA receptor phosphorylation by dopamine and muscarinic receptor agents in the rat medial prefrontal cortex. European journal of pharmacology. 2017 Dec 11;820:45-52.
28578480 not listed Lameth, J., et al. 2017 Acute Neuroinflammation Promotes Cell Responses to 1800 MHz GSM Electromagnetic Fields in the Rat Cerebral Cortex. Neurotoxicity Research, pp.1-16.
27060412 not listed Xue, B., et al. 2017 Integrated regulation of AMPA glutamate receptor phosphorylation in the striatum by dopamine and acetylcholine. Neuropharmacology, 112, pp.57-65.
26706598 not listed O’Leary, H., et al. 2016 Enhanced long term potentiation and decreased AMPA receptor desensitization in the acute period following a single kainate induced early life seizure. Neurobiology of disease, 87, 134-144.
26658861 1:1000 Bean, L.A., et al. 2015 Re-opening the critical window for estrogen therapy. Journal of Neuroscience, 35(49), pp.16077-16093.
25689263 1:1000 Mao, L.M., et al. 2015 Dynamic increases in AMPA receptor phosphorylation in the rat hippocampus in response to amphetamine. Journal of neurochemistry. Jun;133(6):795-805.
24780380 1:500 Bernard, P.B., et al. 2014 Necessary, but not sufficient: insights into the mechanisms of mGluR mediated long-term depression from a rat model of early life seizures. Neuropharmacology, 84, 1-12.
24231469 1:1000 Xue, B., et al. 2014 Rapid and sustained GluA1 S845 phosphorylation in synaptic and extrasynaptic locations in the rat forebrain following amphetamine administration. Neurochemistry international, 64, 48-54.
24056996 1:1000 Barcomb, K., et al. 2013 Enzymatic activity of CaMKII is not required for its interaction with the glutamate receptor subunit GluN2B. Molecular pharmacology, 84(6), 834-843.
20554878 1:500 Sears, R.M., et al. 2010 Regulation of Nucleus Accumbens Activity by the Hypothalamic Neuropeptide Melanin-Concentrating Hormone. J. Neurosci., 30: 8263 – 8273.
20353941 not listed Coultrap, S.J., et al. 2010 CaMKII Autonomy Is Substrate-dependent and Further Stimulated by Ca2+/Calmodulin. J. Biol. Chem., 285: 17930 – 1793.
18819923 1:1000 Davies, K.D., et al. 2008 Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not -Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Internalization. J. Biol. Chem., 283: 33138 – 33146.
18519737 not listed Leal-Ortiz, S., et al. 2008 Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis. J. Cell Biol., 181: 831 – 846.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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