GFAP Antibody, Rabbit

Catalog #: 620-GFAP Categories: , , Datasheet:

$99.00$310.00

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Rabbit polyclonal antibody

Formulation:
Neat serum
Species Tested:
Bovine, Human, Mouse, Pig, Rat
Applications:
WB 1:1000-1:5000IHC 1:1000-1:5000ICC 1:1000-1:5000
Species:
Rabbit
Gene Name:
GFAP
Molecular Weight:
~50 kDa
Cite This Antibody:
PhosphoSolutions Cat# 620-GFAP, RRID:AB_2492124
Antigen/Purification: ExpandCollapse

The antigen is a recombinant and purified bovine GFAP.

The antibody is unpurified neat serum.

Biological Significance: ExpandCollapse

Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and co-workers as a major fibrous protein of multiple sclerosis plaques (1). It was subsequently found to be a member of the 10nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers (2).

Synonyms: ExpandCollapse

• wu:fb34h11 antibody
• ALXDRD antibody
• cb345 antibody
• etID36982.3 antibody
• FLJ42474 antibody
• FLJ45472 antibody
• GFAP antibody
• GFAP_HUMAN antibody
• gfapl antibody
• Glial fibrillary acidic protein antibody
• Intermediate filament protein antibody
• wu:fk42c12 antibody
• xx:af506734 antibody
• zgc:110485 antibody

Storage

100 µl neat serum. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunocytochemistry

Cultured neurons and glia

Immunohistochemistry

Rat Cerebellum

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blotting: Rat Hippocampus


Western Blotting: Rat Hippocampus
PMID Dilution Publication
29916754 1:1000 Homiack, D., et al. 2018. Effect of acute alarm odor exposure and biological sex on generalized avoidance and glutamatergic signaling in the hippocampus of Wistar rats. Stress, Jul;21(4):292-303.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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GFAP Antibody, Rabbit
Western blot of rat cortical lysate showing specific immunolabeling of the ~50 kDa GFAP protein.

Click here to view our Western blotting and lysate preparation protocols.

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