GFAP Antibody, Chicken

Catalog #: 621-GFAP Categories: , Datasheet:

$99.00$310.00

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Strong and specific astroglial cell marker

Formulation:
Total IgY fraction
Species Tested:
Bovine, Human, Mouse, Pig, Rat
Applications:
WB 1:10,000IHC 1:1000-1:5000ICC 1:1000-1:5000
Species:
Chicken
Gene Name:
GFAP
Molecular Weight:
~50 kDa
Cite This Antibody:
PhosphoSolutions Cat# 621-GFAP, RRID:AB_2492125
Antigen/Purification: ExpandCollapse

The antigen is a recombinant and purified bovine GFAP.

The antibody is total IgY fraction.

Biological Significance: ExpandCollapse

Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and co-workers as a major fibrous protein of multiple sclerosis plaques (1). It was subsequently found to be a member of the 10nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers (2).

Synonyms: ExpandCollapse

• wu:fb34h11 antibody
• ALXDRD antibody
• cb345 antibody
• etID36982.3 antibody
• FLJ42474 antibody
• FLJ45472 antibody
• GFAP antibody
• GFAP_HUMAN antibody
• gfapl antibody
• Glial fibrillary acidic protein antibody
• Intermediate filament protein antibody
• wu:fk42c12 antibody
• xx:af506734 antibody
• zgc:110485 antibody

Storage

100 µl total IgY fraction in PBS + 10 mM NaN3. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunocytochemistry

Mixed neurons and glia cultured

Immunohistochemistry

Mouse hippocampus

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Mouse Hippocampus


Immunohistochemistry: Mouse Hippocampus
PMID Dilution Publication
29173281 1:1000 Levenga, J., et al. 2017. AKT isoforms have distinct hippocampal expression and roles in synaptic plasticity. eLife, 6, Nov 27. pii: e30640.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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GFAP Antibody, Chicken
Western blot of rat cortical lysate showing specific immunolabeling of the ~50 kDa GFAP protein.

Click here to view our Western blotting and lysate preparation protocols.
GFAP Antibody, Chicken
Immunolabeling of mixed neuron and glia cultures where astrocytes are strongly and specifically labeled with Anti-GFAP(catalog # 621-GFAP, 1:1000, red), and nuclear staining with DAPI (blue).

Download the protocol used for this image:

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