GFAP Antibody, Chicken

Our GFAP chicken polyclonal total protein antibody is total IgY fraction. Strong and specific astroglial cell marker. Optimized in WB, ICC, and IHC.

Catalog #: 621-GFAP Categories: , , Datasheet:


  • SizePrice
Total IgY fraction
Species Tested:
Bovine, Human, Mouse, Pig, Rat
WB 1:10,000IHC 1:1000-1:5000ICC 1:1000-1:5000 Don't see your application?
Host Species:
Chicken Polyclonal
Gene Name:
Molecular Weight:
~50 kDa
Cite This Antibody:
PhosphoSolutions Cat# 621-GFAP, RRID:AB_2492125
Antigen/Purification: ExpandCollapse

The antigen is a recombinant and purified bovine GFAP.

The antibody is total IgY fraction.

Biological Significance: ExpandCollapse

Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and co-workers as a major fibrous protein of multiple sclerosis plaques (1). It was subsequently found to be a member of the 10nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers (2).

Synonyms: ExpandCollapse

• wu:fb34h11 antibody
• ALXDRD antibody
• cb345 antibody
• etID36982.3 antibody
• FLJ42474 antibody
• FLJ45472 antibody
• GFAP antibody
• GFAP_HUMAN antibody
• gfapl antibody
• Glial fibrillary acidic protein antibody
• Intermediate filament protein antibody
• wu:fk42c12 antibody
• xx:af506734 antibody
• zgc:110485 antibody


100 µl total IgY fraction in PBS + 10 mM NaN3. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols


Mixed neurons and glia cultured


Mouse hippocampus

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Mouse Hippocampus

Immunohistochemistry: Mouse Hippocampus
PMID Dilution Publication
29173281 1:1000 Levenga, J., et al. 2017. AKT isoforms have distinct hippocampal expression and roles in synaptic plasticity. eLife, 6, Nov 27. pii: e30640.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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