Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody

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Our Anti-Glial Fibrillary Acidic Protein (GFAP) primary antibody from PhosphoSolutions is chicken polyclonal. It detects bovine, human, mouse, and rat Glial Fibrillary Acidic Protein (GFAP) and is total IgY fraction. It is great for use in WB, IHC, ICC.

Immunofluorescence of a section of mouse hippocampus colabeled with Anti-GFAP(cat. 621-GFAP, green, 1:5000) and Anti-FOX3 (red). The Anti-FOX3 labels the nuclei and proximal perikarya of neurons while the Anti-GFAP labels a network of astroglial cells. The blue is DAPI staining of nuclear DNA.
Western blot of rat cortical lysate showing specific immunolabeling of the ~50 kDa GFAP protein.
Immunofluorescence of a section of mouse prefrontal cortex labeled with Anti-GFAP(cat. 621-GFAP, red, 1:1000). Image courtesy Andrea Cardenas, Rosalind Franklin Unversity, Medicine and Science.
Immunolabeling of mixed neuron and glia cultures where astrocytes are strongly and specifically labeled with Anti-GFAP(cat. 621-GFAP, 1:1000, red), and nuclear staining with DAPI (blue).
Immunolabeling of cultured rat neurons showing strong nuclear and distal cytoplasmic labeling with anti-FOX3(cat. 583-FOX3, 1:1000, red). The complete absence of astrocyte staining is shown using anti-GFAP (cat. 621-GFAP, 1:1000, green) and nuclear staining was done with DAPI (blue).
Immunofluorescence of a section of rat cerebellum showing specific labeling of MeCP2 (cat. 1205-MeCP2, 1:1000, red) in nuclei of neurons and specific labeling of GFAP (cat. 621-GFAP, 1:5000, green) in the network of astroglial cells and projections of Bergmann glia, and Hoechst staining of nuclear DNA.
Immunofluorescence of a section of rat frontal cortex showing labeling of Neurofilament-L (cat. 1452-NFL, 1:500, red) and labeling of GFAP( cat. 621-GFAP , 1:5000, green). The anti-NFL antibody labels cell bodies and processes of pyramidal neurons, as well as dendrites and axons of other neuronal cells. While the anti-GFAP antibody labels the network of glial cells.
Immunolabeling of E20 rat mixed neuron and glial cultured cells labeled with anti-neuron specific enolase antibody (cat. 1520-NSE, red, 1:500) and anti-GFAP antibody (cat. 621-GFAP, green, 1:5000). The blue is Hoechst stain of nuclear DNA. Anti-NSE labels protein expressed in neuronal cells and anti-GFAP labels protein expressed in intermediate filaments in astrocytic and other glial cells.
Immunostaining of E20 rat cortical neuron/glial cell culture stained with anti-vimentin (cat. 2107-VIM, red, 1:2000) and anti-GFAP antibody (cat. 621-GFAP, green, 1:5000). The blue is DAPI staining nuclear DNA. Vimentin is expressed alone in fibroblastic and developing cells and appear red. The astrocytes that appear red express only GFAP, while the golden yellow and yellow astrocytes express GFAP and Vimentin.
Immunofluorescence of a section of mouse hippocampus colabeled with Anti-GFAP(cat. 621-GFAP, green, 1:5000) and Anti-FOX3 (red). The Anti-FOX3 labels the nuclei and proximal perikarya of neurons while the Anti-GFAP labels a network of astroglial cells. The blue is DAPI staining of nuclear DNA.
Immunofluorescence of a section of mouse hippocampus colabeled with Anti-GFAP(cat. 621-GFAP, green, 1:5000) and Anti-FOX3 (red). The Anti-FOX3 labels the nuclei and proximal perikarya of neurons while the Anti-GFAP labels a network of astroglial cells. The blue is DAPI staining of nuclear DNA.
Western blot of rat cortical lysate showing specific immunolabeling of the ~50 kDa GFAP protein.
Immunofluorescence of a section of mouse prefrontal cortex labeled with Anti-GFAP(cat. 621-GFAP, red, 1:1000). Image courtesy Andrea Cardenas, Rosalind Franklin Unversity, Medicine and Science.
Immunolabeling of mixed neuron and glia cultures where astrocytes are strongly and specifically labeled with Anti-GFAP(cat. 621-GFAP, 1:1000, red), and nuclear staining with DAPI (blue).
Immunolabeling of cultured rat neurons showing strong nuclear and distal cytoplasmic labeling with anti-FOX3(cat. 583-FOX3, 1:1000, red). The complete absence of astrocyte staining is shown using anti-GFAP (cat. 621-GFAP, 1:1000, green) and nuclear staining was done with DAPI (blue).
Immunofluorescence of a section of rat cerebellum showing specific labeling of MeCP2 (cat. 1205-MeCP2, 1:1000, red) in nuclei of neurons and specific labeling of GFAP (cat. 621-GFAP, 1:5000, green) in the network of astroglial cells and projections of Bergmann glia, and Hoechst staining of nuclear DNA.
Immunofluorescence of a section of rat frontal cortex showing labeling of Neurofilament-L (cat. 1452-NFL, 1:500, red) and labeling of GFAP( cat. 621-GFAP , 1:5000, green). The anti-NFL antibody labels cell bodies and processes of pyramidal neurons, as well as dendrites and axons of other neuronal cells. While the anti-GFAP antibody labels the network of glial cells.
Immunolabeling of E20 rat mixed neuron and glial cultured cells labeled with anti-neuron specific enolase antibody (cat. 1520-NSE, red, 1:500) and anti-GFAP antibody (cat. 621-GFAP, green, 1:5000). The blue is Hoechst stain of nuclear DNA. Anti-NSE labels protein expressed in neuronal cells and anti-GFAP labels protein expressed in intermediate filaments in astrocytic and other glial cells.
Immunostaining of E20 rat cortical neuron/glial cell culture stained with anti-vimentin (cat. 2107-VIM, red, 1:2000) and anti-GFAP antibody (cat. 621-GFAP, green, 1:5000). The blue is DAPI staining nuclear DNA. Vimentin is expressed alone in fibroblastic and developing cells and appear red. The astrocytes that appear red express only GFAP, while the golden yellow and yellow astrocytes express GFAP and Vimentin.
Rat
ICC, IHC, WB
Chicken

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from PhosphoSolutions Datasheet
Trial Size Trial Size

SKU: 621-GFAP

Volume: 100 µL
Price:
$375

Product Specific References for Applications and Species

Immunohistochemistry: Human
PMID Dilution Publication
PMID Dilution Publication
34296180 1:250 Levenga, J., et al. 2021. Immunohistological Examination of AKT Isoforms in the Brain: Cell-Type Specificity that May Underlie AKT’s Role in Complex Brain Disorders and Neurological Disease. Cerebral Cortex Communications.
Immunohistochemistry: Mouse
PMID Dilution Publication
PMID Dilution Publication
364432491:1000Milstead, R.A., et al. 2022. TDP-43 Knockdown In Mouse Model Of ALS Leads To Dsrna Deposition, Gliosis, And Neurodegeneration In The Spinal Cord. Cerebral Cortex, .
34296180 1:1000 Levenga, J., et al. 2021. Immunohistological Examination of AKT Isoforms in the Brain: Cell-Type Specificity that May Underlie AKT’s Role in Complex Brain Disorders and Neurological Disease. Cerebral Cortex Communications.
29173281 1:1000 Levenga, J., et al. 2017. AKT isoforms have distinct hippocampal expression and roles in synaptic plasticity. eLife, 6, Nov 27. pii: e30640.

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