GAP43 Antibody

Catalog #: 875-GAP43 Categories: , , Datasheet:

$99.00$310.00

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Great for staining the plasma membrane of neurons!

Formulation:
Total IgY fraction
Species Tested:
Bovine, Human, Mouse, Pig, Rat
Applications:
WB 1:2000IHC 1:500-1:1000ICC 1:500-1:2000
Species:
Chicken polyclonal
Gene Name:
GAP43
Molecular Reference:
~43 kDa
Cite This Antibody:
PhosphoSolutions Cat# 875-GAP43, RRID:AB_2492122
Antigen/Purification: ExpandCollapse

The antigen is a synthetic peptide corresponding to amino acid residues from the C-terminal region of the rat GAP43 sequence.

The antibody is total IgY fraction.

Biological Significance: ExpandCollapse

GAP-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that GAP-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). GAP-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by protein kinase C modulates the interaction of GAP-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002).

Synonyms: ExpandCollapse

• Axonal membrane protein GAP 43 antibody
• Axonal membrane protein GAP-43 antibody
• B 50 antibody
• Calmodulin binding protein P 57 antibody
• F1 antibody
• GAP 43 antibody
• GAP-43 antibody
• GAP43 antibody
• Growth Associated Protein 43 antibody
• Growth-associated protein 43 antibody
• Nerve Growth Related Peptide antibody
• Nerve growth related peptide GAP43 antibody
• NEUM_HUMAN antibody
• Neural phosphoprotein B 50 antibody
• Neural phosphoprotein B-50 antibody
• Neuromodulin antibody
• Neuron growth associated protein 43 antibody
• PP46 antibody
• Protein F1 antibody
• QtrA-11580 antibody
• QtrA-13071 antibody

Storage

100 µl total IgY fraction in PBS + 10 mM NaN3. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunocytochemistry

Rat neuron and glial culture

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Benowitz LI, Routtenberg A (1997) Gap-43: An intrinsic determinant of neuronal development and plasticit. Trends Neurosci 20:84-91.

He, Q, Dent, EW, Meiri, KF (1997) Modulation of actin filament behavior by Gap-43 (neuromodulin) is dependent on the phosphorylation status of serine 41, the protein kinase C site. J Neurosci 17:3515-3524.

Hulo S, Alberi, S, Laux T, Muller D, Caroni P (2002) A point mutant of Gap-43 induces enhanced short-term and long term hippocampal plasticity. Eur J Neurosci 15:1976-1982.

Strittmatter SM, Fankhauser C, Huang PL, Mashimo H, Fishman MC (1995) Neuronal pathfinding is abnormal in mice lacking the neuronal growth cone protein Gap-43,” Cell 80:445-452.

Rayudu Gopalakrishna, Usha Gundimeda, Jason Eric Schiffman, and Thomas H. McNeill (2008) A Direct Redox Regulation of Protein Kinase C Isoenzymes Mediates Oxidant-induced Neuritogenesis in PC12 Cells J. Biol. Chem., May 2008; 283: 14430 – 14444.

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GAP43 Antibody
Western blot of rat cortical lysate showing specific immunolabeling of the ~43 kDa GAP43 protein.

Click here to view our Western blotting and lysate preparation protocols.

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