Affinity purified from pooled serum. Learn more.

Gap-43 (Ser41) Antibody

We are the original manufacturer of our Gap-43 (Ser41) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. Optimized in WB.

Catalog #: p1150-41 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Expected Reactivity:
Bovine, Canine, Chicken, Human, Non-human primate, Xenopus, Zebra fish
Applications:
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
GAP43
Molecular Weight:
~50 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1150-41, RRID:AB_2492123
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser41 of Gap-43.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Gap-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that Gap-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). Gap-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser 41 by protein kinase C (Catalog No. 1609-PKC) modulates the interaction of Gap-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002).

Synonyms: ExpandCollapse

• Axonal membrane protein GAP 43 antibody
• Axonal membrane protein GAP-43 antibody
• B 50 antibody
• Calmodulin binding protein P 57 antibody
• F1 antibody
• GAP 43 antibody
• GAP-43 antibody
• GAP43 antibody
• Growth Associated Protein 43 antibody
• Growth-associated protein 43 antibody
• Nerve Growth Related Peptide antibody
• Nerve growth related peptide GAP43 antibody
• NEUM_HUMAN antibody
• Neural phosphoprotein B 50 antibody
• Neural phosphoprotein B-50 antibody
• Neuromodulin antibody
• Neuron growth associated protein 43 antibody
• PP46 antibody
• Protein F1 antibody
• QtrA-11580 antibody
• QtrA-13071 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Rat


Western Blot: Rat
PMID Dilution Publication
18375950 1:1000 Gopalakrishna, R., et al. 2008 A direct redox regulation of protein kinase C isoenzymes mediates oxidant-induced neuritogenesis in PC12 cells. Journal of Biological Chemistry, 283(21), pp.14430-14444.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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