Affinity purified from pooled serum. Learn more.

Gap-43 (Ser41) Antibody

Catalog #: p1150-41 Category: Datasheet:

$365.00

Rabbit polyclonal antibody

Pooled Serum
Size:
100 µl
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Rat, Mouse, Human, Xenopus, Bovine, Chicken, Non-human primate, Zebra fish, Canine
Applications:
WB 1:1,000
Species:
Rabbit
Molecular Reference:
~50 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1150-41, RRID:AB_2492123
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser41 of Gap-43.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Gap-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that Gap-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). Gap-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser 41 by protein kinase C (Catalog No. 1609-PKC) modulates the interaction of Gap-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Rayudu Gopalakrishna, Usha Gundimeda, Jason Eric Schiffman, and Thomas H. McNeill (2008) A Direct Redox Regulation of Protein Kinase C Isoenzymes Mediates Oxidant-induced Neuritogenesis in PC12 Cells J. Biol. Chem., May 2008; 283: 14430 – 14444. PMID: 18375950

Gap-43 ser41 Antibody
Western blot of rat cortical lysate showing specific immunolabeling of the ~50 kDa Gap-43 protein phosphorylated at Ser41 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

You may also like…