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GAD-67 (Glutamic Acid Decarboxylase, 67 kDa) Antibody

Chicken Polyclonal Antibody

Catalog #: GAD Categories: , , , , Datasheet:

$399.00

Size:
1000 µl
Formulation:
Affinity Purified IgY
Species Tested:
Mouse
Expected Reactivity:
Human, Mouse, Rat
Applications:
WB 1:2000-1:5000IHC 1:1000-1:2000ICC 1:1000-1:2000
Host Species:
Chicken Polyclonal
Gene Name:
GAD1 GAD GAD67
Molecular Weight:
67 kDa
Cite This Antibody:
Aves Labs Cat# GAD, RRID:AB_2313545
Antigen/Purification: ExpandCollapse

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to a region near the Cterminus of this gene product, and was 100% conserved between the human (Q99259, NCBI), mouse (P48318, NCBI) and rat (NP_058703, NCBI) gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 µg/mL, and the preparation was filter-sterilized through a 0.45 µm filter.

Synonyms: ExpandCollapse
  • Glutamate decarboxylase 1 EC 4.1.1.15
  • 67 kDa glutamic acid decarboxylase
  • GAD-67
  • Glutamate decarboxylase 67 kDa isoform

Storage

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS,” pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Product Specific References

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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