Affinity purified from pooled serum. Learn more.

GABAA Receptor β1 Antibody

Our rabbit polyclonal GABAA Receptor β1 total protein antibody is affinity purified from pooled serum. It is excellent for use in WB.

Catalog #: 860A-GB1C** Categories: , Datasheet:

$119.00$380.00

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**This antibody is a replacement for our original catalog #860-GB1C. It was produced by the same methods, using the same fusion protein antigen in new animals.

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Rat, Mouse
Expected Reactivity:
Bovine, Canine, Chicken, Human, Non-human primate, Xenopus, Zebra fish
Applications:
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
GABRB1
Molecular Weight:
~55 kDa
Cite This Antibody:
PhosphoSolutions Cat# 860A-GB1C, RRID:AB_2617129
Antigen/Purification: ExpandCollapse

The antigen is a fusion protein from the cytoplasmic loop of the β1-subunit of rat GABAA receptor.

The antibody is prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Synonyms: ExpandCollapse

• AW061132 antibody
• B230208N19Rik antibody
• GABA(A) receptor beta 1 antibody
• GABA(A) receptor subunit beta-1 antibody
• GABA-A receptor, beta-1 polypeptide antibody
• Gabrb-1 antibody
• GABRB1 antibody
• Gamma aminobutyric acid (GABA) A receptor beta 1 antibody
• Gamma Aminobutyric Acid A Receptor Beta 1 antibody
• Gamma Aminobutyric Acid Receptor , beta-1 antibody
• Gamma-aminobutyric acid (GABA) A receptor, subunit beta 1 antibody
• Gamma-aminobutyric acid receptor subunit beta-1 antibody
• GARB1 antibody
• GBRB1_HUMAN antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Brandon NJ, et al. (2003) A kinase anchoring protein 79/150 facilitates the phosphorylation of GABAA receptors by cAMP-dependent protein kinase via selective interaction with receptor β-subunits. Mol Cell Neurosci 22:87-97. PMID: 12595241

McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor α1-subtype. Nature Neurosci 3:587-592. PMID: 10816315

Mehta AK, Ticku MK (1998) Prevalence of the GABAA receptor assemblies containing α1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration. Mol Brain Res 67:194-199. PMID: 10101248

Ogris W, et al.(2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor γ2-subunit. Biochem Pharmacol 68:1621-1629. Olsen RW, Tobin AJ (1990) Molecular biology of GABAA receptors. FASEB 4:1469-1480. PMID: 15451405

Pöltl A, et al. (2003) Subunit composition and quantitative importance of GABAA receptor subtypes in the cerebellum of mouse and rat. J Neurochem 87:1444-1455. PMID: 14713300

Whiting PJ, et al. (1999) Molecular and functional diversity of the expanding GABAA receptor gene family. Ann NY Acad Sci 868:645-653. PMID: 10414349

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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