Affinity purified from pooled serum. Learn more.

GABAA Receptor β1 Antibody

Catalog #: 860A-GB1C** Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

**This antibody is a replacement for our original catalog # 860-GB1C.
It was produced by the same methods, using the same fusion protein
antigen in new animals.

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Rat, Mouse
Species Reactivity:
Bovine, Canine, Chicken, Human, Non-human primate, Xenopus, Zebra fish
Applications:
WB 1:1000
Species:
Rabbit Polyclonal
Gene Name:
GABRB1
Molecular Weight:
~55 kDa
Cite This Antibody:
PhosphoSolutions Cat# 860A-GB1C, RRID:AB_2617129
Antigen/Purification: ExpandCollapse

The antigen is a fusion protein from the cytoplasmic loop of the β1-subunit of rat GABAA receptor.

The antibody is prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Synonyms: ExpandCollapse

• AW061132 antibody
• B230208N19Rik antibody
• GABA(A) receptor beta 1 antibody
• GABA(A) receptor subunit beta-1 antibody
• GABA-A receptor, beta-1 polypeptide antibody
• Gabrb-1 antibody
• GABRB1 antibody
• Gamma aminobutyric acid (GABA) A receptor beta 1 antibody
• Gamma Aminobutyric Acid A Receptor Beta 1 antibody
• Gamma Aminobutyric Acid Receptor , beta-1 antibody
• Gamma-aminobutyric acid (GABA) A receptor, subunit beta 1 antibody
• Gamma-aminobutyric acid receptor subunit beta-1 antibody
• GARB1 antibody
• GBRB1_HUMAN antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Brandon NJ, et al. (2003) A kinase anchoring protein 79/150 facilitates the phosphorylation of GABAA receptors by cAMP-dependent protein kinase via selective interaction with receptor β-subunits. Mol Cell Neurosci 22:87-97. PMID: 12595241

McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor α1-subtype. Nature Neurosci 3:587-592. PMID: 10816315

Mehta AK, Ticku MK (1998) Prevalence of the GABAA receptor assemblies containing α1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration. Mol Brain Res 67:194-199. PMID: 10101248

Ogris W, et al.(2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor γ2-subunit. Biochem Pharmacol 68:1621-1629. Olsen RW, Tobin AJ (1990) Molecular biology of GABAA receptors. FASEB 4:1469-1480. PMID: 15451405

Pöltl A, et al. (2003) Subunit composition and quantitative importance of GABAA receptor subtypes in the cerebellum of mouse and rat. J Neurochem 87:1444-1455. PMID: 14713300

Whiting PJ, et al. (1999) Molecular and functional diversity of the expanding GABAA receptor gene family. Ann NY Acad Sci 868:645-653. PMID: 10414349

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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GABA A Receptor β1 Antibody
Western blot of mouse whole brain lysates showing specific immunolabeling of the ~55 kDa β1-subunit of the GABAA-R.

Click here to view our Western blotting and lysate preparation protocols.

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