GABA Transporter (GAT) 2 Antibody

Catalog #: 881-GAT2 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Formulation:
Affinity purified
Species Tested:
Rat, Mouse
Applications:
WB 1:100-200IHC 1:100-200
Species:
Rabbit
Gene Name:
SLC6A13
Molecular Weight:
~67 kDa
Cite This Antibody:
PhosphoSolutions Cat# 881-GAT2, RRID:AB_2492120
Antigen/Purification: ExpandCollapse

The antigen is a peptide corresponding to amino acid residues from the C-terminal region of rat GAT-2.

The antibody is prepared from rabbit serum by affinity purification via chromatography with a column made with the peptide used as antigen.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl channel associated with the GABAA receptor (GABAA-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by high affinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-2 is found in a wide range of neuronal and non-neuronal cells including dendrites and axon terminals as well as epithelial cells and cells forming the pia and arachnoid complex (Conti et al., 1999)

Synonyms: ExpandCollapse

• GABA transport protein antibody
• GAT 2 antibody
• GAT-2 antibody
• GAT2 antibody
• GAT3 antibody
• S6A13_HUMAN antibody
• SLC6A13 antibody
• Sodium- and chloride-dependent GABA transporter 2 antibody
• Solute carrier family 6 (Neurotransmitter transporter, GABA), member 13 antibody
• Solute carrier family 6 member 13 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunohistochemistry

Rat retina

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Rat


Immunohistochemistry: Rat
PMID Dilution Publication
8915826 not listed Johnson, J., et al. 1997. Multiple γ-aminobutyric acid plasma membrane transporters (GAT-1, GAT-2, GAT-3) in the rat retina. The Journal of comparative neurology, 375(2), 212.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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GAT2 Western Blot Image
Western blot of mouse whole brain lysate showing specific immunolabeling of the ~67 kDa GAT-2 protein.

Click here to view our Western blotting and lysate preparation protocols.

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