GABA Transporter (GAT) 1 Antibody

Catalog #: 880-GAT1 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Formulation:
Affinity purified
100% Homology:
Rat, Mouse
Applications:
WB 1:1,000IHC 1:100-1:200
Species:
Rabbit
Gene Name:
SLC6A1
Molecular Reference:
~67 kDa
Cite This Antibody:
PhosphoSolutions Cat# 880-GAT1, RRID:AB_2492119
Antigen/Purification: ExpandCollapse

The antigen is a peptide corresponding to amino acid residues from the C-terminal region of rat GAT-1.

The antibody is prepared from rabbit serum by affinity purification via chromatography with a column made with the peptide used as antigen.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABAA receptor (GABAA-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by highaffinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-1, the most abundant of the transporters, is found predominantly in neurons, but also in some specialized glia (Minelli et al., 1995). GAT-1 is thought to play a key role in epileptogenesis (Zhao et al. 2003).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific Reference

Johnson, J., Chen, T. K., Rickman, D. W., Evans, C., & Brecha, N. C. (1996). Multiple γ-aminobutyric acid plasma membrane transporters (GAT-1, GAT-2, GAT-3) in the rat retina. The Journal of comparative neurology, 375(2), 212. PMID: 8915826

GABA Transporter (GAT) 1 Antibody
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~67 kDa GAT-1 protein.

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