GABA Transporter (GAT) 1 Antibody

Catalog #: 880-GAT1 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Formulation:
Affinity purified
Species Tested:
Mouse, Human, Non-human Primate, Rat, Salamander
Applications:
WB 1:1000IHC 1:100-1:1000
Species:
Rabbit
Gene Name:
SLC6A1
Molecular Weight:
~67 kDa
Cite This Antibody:
PhosphoSolutions Cat# 880-GAT1, RRID:AB_2492119
Antigen/Purification: ExpandCollapse

The antigen is a peptide corresponding to amino acid residues from the C-terminal region of rat GAT-1.

The antibody is prepared from rabbit serum by affinity purification via chromatography with a column made with the peptide used as antigen.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl channel associated with the GABAA receptor (GABAA-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by high affinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-1, the most abundant of the transporters, is found predominantly in neurons, but also in some specialized glia (Minelli et al., 1995). GAT-1 is thought to play a key role in epileptogenesis (Zhao et al. 2003).

Synonyms: ExpandCollapse

• GABATHG antibody
• GABATR antibody
• GABT 1 antibody
• GABT1 antibody
• GAT-1 antibody
• GAT1 antibody
• SC6A1_HUMAN antibody
• Slc6a1 antibody
• Sodium and chloride dependent GABA transporter 1 antibody
• Sodium- and chloride-dependent GABA transporter 1 antibody
• Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody
• Solute carrier family 6 member 1 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunohistochemistry

Rat retina

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Human | Non-Human Primate| Rat | Salamanders


Immunohistochemistry: Human
PMID Dilution Publication
9623887 1:100 Conti, F., et al. 1998. Neuronal and glial localization of GAT‐1, a high‐affinity γ‐aminobutyric acid plasma membrane transporter, in human cerebral cortex: With a note on its distribution in monkey cortex. Journal of Comparative Neurology, 396(1), pp.51-63.


Immunohistochemistry: Non-Human Primate
PMID Dilution Publication
9623887 1:100 Conti, F., et al. 1998. Neuronal and glial localization of GAT‐1, a high‐affinity γ‐aminobutyric acid plasma membrane transporter, in human cerebral cortex: With a note on its distribution in monkey cortex. Journal of Comparative Neurology, 396(1), pp.51-63.


Immunohistochemistry: Rat
PMID Dilution Publication
8915826 not listed Johnson, J., et al. 1997. Multiple γ-aminobutyric acid plasma membrane transporters (GAT-1, GAT-2, GAT-3) in the rat retina. The Journal of comparative neurology, 375(2), 212.


Immunohistochemistry: Salamander
PMID Dilution Publication
9390763 1:1000 Yang, C.Y., et al. 1997. Immunocytochemical localization of γ‐aminobutyric acid plasma membrane transporters in the tiger salamander retina. Journal of Comparative Neurology, 389(1), pp.117-126.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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GABA Transporter (GAT) 1 Antibody
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~67 kDa GAT-1 protein.

Click here to view our Western blotting and lysate preparation protocols.
Immunoflouresence of mouse retina specifically labeling GAT-1 protein (cat. #880-GAT1, red, 1:500) in the inner plexiform layer (IPL). The white identifies nuclei stained with DAPI. The retina was fixed with 4% paraformaldehyde for 30 minutes, embedded in 5% agarose, and sliced into 60 micron thick slices with a vibratome. The image is a Z-stack taken from a confocal microscope. Image courtesy of Andrea Wellington, Assistant Research Scientist, University of Arizona Physiology Dept.

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