Our Anti-GABA Transporter (GAT) 1 primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, non-human primate, and rat GABA Transporter (GAT) 1 and is affinity purified. It is great for use in WB, IHC.
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~67 kDa GAT-1 protein.
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by high affinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-1, the most abundant of the transporters, is found predominantly in neurons, but also in some specialized glia (Minelli et al., 1995). GAT-1 is thought to play a key role in epileptogenesis (Zhao et al. 2003).
Affinity Purified
Polyclonal
IgG
IHC, WB
Rabbit
SLC6A1
67 kDa
Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat GAT1, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
AB_2492119
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
IHC: 1:100-1:1000
Unconjugated
Specific for endogenous levels of the ~67 kDa GAT-1 protein. Immunolabeling is blocked by pre-adsorption of antibody with the peptide used to generate the antibody.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
GABATHG antibody, GABATR antibody, GABT 1 antibody, GABT1 antibody, GAT-1 antibody, GAT1 antibody, SC6A1_HUMAN antibody, Slc6a1 antibody, Sodium and chloride dependent GABA transporter 1 antibody, Sodium- and chloride-dependent GABA transporter 1 antibody, Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody, Solute carrier family 6 member 1 antibody
UniProt (Immunogen Species): P23978
79212
Product Specific References for Applications and Species
Conti, F., et al. 1998. Neuronal and glial localization of GAT‐1, a high‐affinity γ‐aminobutyric acid plasma membrane transporter, in human cerebral cortex: With a note on its distribution in monkey cortex. Journal of Comparative Neurology, 396(1), pp.51-63.
Conti, F., et al. 1998. Neuronal and glial localization of GAT‐1, a high‐affinity γ‐aminobutyric acid plasma membrane transporter, in human cerebral cortex: With a note on its distribution in monkey cortex. Journal of Comparative Neurology, 396(1), pp.51-63.
Johnson, J., et al. 1997. Multiple γ-aminobutyric acid plasma membrane transporters (GAT-1, GAT-2, GAT-3) in the rat retina. The Journal of Comparative Neurology, 375(2), 212.
Yang, C.Y., et al. 1997. Immunocytochemical localization of γ‐aminobutyric acid plasma membrane transporters in the tiger salamander retina. Journal of Comparative Neurology, 389(1), pp.117-126.
