Affinity purified from pooled serum. Learn more.

GABAA Receptor β3 (Ser408/409) Antibody

Catalog #: p1130-4089 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Rat, Mouse
Species Reactivity:
Bovine, Canine, Chicken, Human, Non-human primate, Xenopus, Zebra fish
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
GABRB3
Molecular Weight:
~58 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1130-4089, RRID:AB_2492111
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the di-phospho-Ser408/409 of the rat GABAA receptor, β3 subunit.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. Phosphorylation of serine 408 and serine 409 within the β3 subunit have been shown to be critical for the functional modulation of β3 containing recombinant receptors (Brandon et al., 2000).

Synonyms: ExpandCollapse

• ECA5 antibody
• GABA alpha receptor beta-2 subunit antibody
• GABA(A) receptor subunit beta-3 antibody
• GABAA receptor beta 3 subunit antibody
• GABAA receptor subunit beta 3 antibody
• GABR B3 antibody
• Gabrb3 antibody
• Gamma aminobutyric acid (GABA) A receptor beta 3 antibody
• Gamma aminobutyric acid receptor subunit beta 3 antibody
• Gamma-aminobutyric acid receptor subunit beta-3 antibody
• GBRB3_HUMAN antibody
• MGC9051 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Mouse | Rat


Western Blot: Mouse
PMID Dilution Publication
30077648 not listed Brewer,A.L., et al. 2018. Role of spinal GABA receptors in the acute antinociceptive response of mice to hyperbaric oxygen. Brain research, 1699, pp.107-116.


Western Blot: Rat
PMID Dilution Publication
25274828 not listed Riffault, B., et al. 2014. Pro-Brain-Derived Neurotrophic Factor Inhibits GABAergic Neurotransmission by Activating Endocytosis and Repression of GABAA Receptors. The Journal of Neuroscience, 34(40), 13516-13534.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~58 kDa GABAA β3 protein phosphorylated at Ser408/409 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Click here to view our Western blotting and lysate preparation protocols.

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