Affinity purified from pooled serum. Learn more.

GABAA Receptor α5 Antibody

Our rabbit polyclonal GABAA Receptor α5 total protein antibody is affinity purified from pooled serum. It is excellent for use in WB and IHC.

Catalog #: 846A-GA5C** Categories: , Datasheet:

$119.00$380.00

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** This antibody is a replacement for our original catalog # 846-GA5C. It was produced by the same methods, using the same fusion protein antigen in new animals.

Pooled Serum
Size:
100 ul
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Rat, Mouse
Expected Reactivity:
Bovine, Canine, Chicken, Human, Non-human Primate, Zebrafish
Applications:
WB 1:1000IHC Engin et al, 2015 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
GABRA5
Molecular Weight:
~55 kDa
Cite This Antibody:
PhosphoSolutions Cat# 846A-GA5C, RRID:AB_2797145
Antigen/Purification: ExpandCollapse

The antigen is a fusion protein from the cytoplasmic loop of the α5-subunit of rat GABAA receptor.

The antibody is prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl channel associated with the GABAreceptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Synonyms: ExpandCollapse

• GAA 5 antibody
• GAA5 antibody
• GABA(A) receptor subunit alpha-5 antibody
• GABRA 5 antibody
• Gabra5 antibody
• Gamma aminobutyric acid GABA A receptor alpha 5 antibody
• Gamma aminobutyric acid GABA A receptor alpha 5 precursor antibody
• Gamma aminobutyric acid receptor alpha 5 subunit precursor GABA A receptor antibody
• Gamma-aminobutyric acid receptor subunit alpha-5 antibody
• GBRA5_HUMAN antibody
• GC138184 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Mouse | Rat
Western Blotting: Mouse


Immunohistochemistry: Mouse
PMID Dilution Publication
26446222*** not listed Engin, E., et al. 2015. Tonic Inhibitory Control of Dentate Gyrus Granule Cells by ?5-Containing GABAA Receptors Reduces Memory Interference. The Journal of Neuroscience, 35(40), 13698-13712.


Western Blot: Mouse
PMID Dilution Publication
30878595*** not listed Yu, J., et al. 2019. Gabapentin increases expression of δ subunit-containing GABAA receptors. EBioMedicine, pii: S2352-3964(19)30148-3.
26446222*** not listed Engin, E., et al. 2015. Tonic Inhibitory Control of Dentate Gyrus Granule Cells by ?5-Containing GABAA Receptors Reduces Memory Interference. The Journal of Neuroscience, 35(40), 13698-13712.


Western Blot: Rat
PMID Dilution Publication
25421517*** 1:1000 Centanni, S.W., et al. 2014. Adolescent alcohol exposure alters GABAA receptor subunit expression in adult hippocampus. Alcoholism: Clinical and Experimental Research, 38(11), 2800-2808.

***Product specific references for previous product # 845-GA4C which has been depleted and replaced with our product # 845A- GA4C which was produced by the same methods, using the same fusion protein antigen in new animals.

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