GABAA Receptor α5 Antibody

Catalog #: 846-GA5C Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Formulation:
Affinity purified
100% Homology:
Rat, Mouse
Applications:
WB 1:1,000
Species:
Rabbit
Gene Name:
GABRA5
Molecular Reference:
~55 kDa
Cite This Antibody:
PhosphoSolutions Cat# 846-GA5C, RRID:AB_2492105
Antigen/Purification: ExpandCollapse

The antigen is a fusion protein from the cytoplasmic loop of the α5-subunit of rat GABAA receptor.

The antibody is prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABAreceptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Engin, E., Zarnowska, E. D., Benke, D., Tsvetkov, E., Sigal, M., Keist, R., Bolshakov, V.Y., Pearce, R.A., & Rudolph, U. (2015). Tonic Inhibitory Control of Dentate Gyrus Granule Cells by α5-Containing GABAA Receptors Reduces Memory Interference. The Journal of Neuroscience, 35(40), 13698-13712. PMID: 26446222

Centanni, S. W., Teppen, T., Risher, M. L., Fleming, R. L., Moss, J. L., Acheson, S. K., Mulholland, P.J., Pandey, S.C., Chandler, L.J., & Swartzwelder, H. S. (2014). Adolescent alcohol exposure alters GABAA receptor subunit expression in adult hippocampus. Alcoholism: Clinical and Experimental Research, 38(11), 2800-2808. PMID: 25421517

Zurek, A. A., Yu, J., Wang, D. S., Haffey, S. C., Bridgwater, E. M., Penna, A., Lecker, I., Lei, G., Chang, T., Salter, E. W. and Orser, B. A. (2014). Sustained increase in α5GABAA receptor function impairs memory after anesthesia. The Journal of clinical investigation, 124(12), p.5437.  PMID: 25365226

GABA A Receptor alpha 5 Antibody
Western blot of 7 µg of rat hippocampal showing specific immunolabeling of the ~55 kDa α5-subunit of the GABAA-R.

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