Affinity purified from pooled serum. Learn more.

GABAA Receptor α5 Antibody

Catalog #: 846A-GA5C* Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

* This antibody is a replacement for our original catalog # 846-GA5C.  It was
produced by the same methods, using the same fusion protein antigen
in new animals.

Pooled Serum
Size:
100 ul
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Rat, Mouse
Species Reactivity:
Bovine, Canine, Chicken, Human, Non-human Primate, Zebrafish
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
GABRA5
Molecular Reference:
~55 kDa
Cite This Antibody:
PhosphoSolutions Cat# 846A-GA5C, RRID:AB_2797145
Antigen/Purification: ExpandCollapse

The antigen is a fusion protein from the cytoplasmic loop of the ?5-subunit of rat GABAA receptor.

The antibody is prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl channel associated with the GABAreceptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Synonyms: ExpandCollapse

• GAA 5 antibody
• GAA5 antibody
• GABA(A) receptor subunit alpha-5 antibody
• GABRA 5 antibody
• Gabra5 antibody
• Gamma aminobutyric acid GABA A receptor alpha 5 antibody
• Gamma aminobutyric acid GABA A receptor alpha 5 precursor antibody
• Gamma aminobutyric acid receptor alpha 5 subunit precursor GABA A receptor antibody
• Gamma-aminobutyric acid receptor subunit alpha-5 antibody
• GBRA5_HUMAN antibody
• GC138184 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References for previous cat. #846-GA5C *

* Our original antibody, cat. #846-GA5C has been depleted and replaced with our new version, cat. #846A-GA5C. It was produced by the same methods, using the same fusion protein antigen in new animals.

Yu, J., Wang, D.S., Bonin, R.P., Penna, A., Alavian-Ghavanini, A., Zurek, A.A., Rauw, G., Baker, G.B. and Orser, B.A., 2019. Gabapentin increases expression of ? subunit-containing GABAA receptors. EBioMedicine, pii: S2352-3964(19)30148-3. PMID: 30878595

Engin, E., Zarnowska, E. D., Benke, D., Tsvetkov, E., Sigal, M., Keist, R., Bolshakov, V.Y., Pearce, R.A., & Rudolph, U. (2015). Tonic Inhibitory Control of Dentate Gyrus Granule Cells by ?5-Containing GABAA Receptors Reduces Memory Interference. The Journal of Neuroscience, 35(40), 13698-13712. PMID: 26446222

Centanni, S. W., Teppen, T., Risher, M. L., Fleming, R. L., Moss, J. L., Acheson, S. K., Mulholland, P.J., Pandey, S.C., Chandler, L.J., & Swartzwelder, H. S. (2014). Adolescent alcohol exposure alters GABAA receptor subunit expression in adult hippocampus. Alcoholism: Clinical and Experimental Research, 38(11), 2800-2808. PMID: 25421517

Zurek, A. A., Yu, J., Wang, D. S., Haffey, S. C., Bridgwater, E. M., Penna, A., Lecker, I., Lei, G., Chang, T., Salter, E. W. and Orser, B. A. (2014). Sustained increase in ?5GABAA receptor function impairs memory after anesthesia. The Journal of clinical investigation, 124(12), p.5437.  PMID: 25365226

Western blot of mouse whole brain showing specific immunolabeling of the ~55 kDa ?5-subunit of the GABAA-R.

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