GABAA Receptor α4 Antibody

Catalog #: 845-GA4C Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Formulation:
Affinity purified
100% Homology:
Rat
Applications:
WB 1:1,000
Species:
Rabbit
Gene Name:
GABRA4
Molecular Reference:
~64 kDa
Cite This Antibody:
PhosphoSolutions Cat# 845-GA4C, RRID:AB_2492103
Antigen/Purification: ExpandCollapse

The antigen is a fusion protein from the cytoplasmic loop of the α4-subunit of rat GABAA receptor.

The antibody is prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Wang, L., Kloc, M., Maher, E., Erisir, A. and Maffei, A., 2018. Presynaptic GABAA Receptors Modulate Thalamocortical Inputs in Layer 4 of Rat V1. Cerebral Cortex. PMID: 29373653

Marowsky A, Rudolph U, Fritschy JM, Arand M (2012) Tonic inhibition in principal cells of the amygdala: a central role for alpha-3 subunit-containing GABAA receptors. J Neurosci. 32(25):8611-9. PMID: 22723702

GABA A Receptor alpha 4 Antibody
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~64 kDa α4-subunit of the GABAA-R.

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