Affinity purified from pooled serum. Learn more.

GABAA Receptor α4 Antibody

Catalog #: 845A-GA4C** Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

**This antibody is a replacement for our original catalog #845-GA4C. It was
produced by the same methods, using the same fusion protein antigen in
new animals.

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Applications:
WB 1:1000IHC 1:300
Species:
Rabbit
Gene Name:
GABRA4
Molecular Weight:
~64 kDa
Cite This Antibody:
PhosphoSolutions Cat# 845A-GA4C, RRID:AB_2800407
Antigen/Purification: ExpandCollapse

The antigen is a fusion protein from the cytoplasmic loop of the α4-subunit of rat GABAA receptor.

The antibody is prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunitsresults in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Synonyms: ExpandCollapse

• GABA(A) receptor subunit alpha 4 antibody
• GABA(A) receptor subunit alpha-4 antibody
• GABR A4 antibody
• GABRA 4 antibody
• Gabra4 antibody
• Gamma aminobutyric acid (GABA) A receptor alpha 4 antibody
• Gamma aminobutyric acid A receptor alpha 4 antibody
• Gamma aminobutyric acid receptor alpha 4 subunit antibody
• Gamma aminobutyric acid receptor subunit alpha 4 antibody
• Gamma-aminobutyric acid receptor subunit alpha-4 antibody
• GBRA4_HUMAN antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Mouse | Rat


Immunohistochemistry: Mouse
PMID Dilution Publication
22723702*** not listed Marowsky, A., et al. 2012. Tonic inhibition in principal cells of the amygdala: a central role for alpha-3 subunit-containing GABAA receptors. J Neurosci. 32(25):8611-9.


Immunohistochemistry: Rat
PMID Dilution Publication
29373653*** 1:300 Wang, L., et al. 2019. . Presynaptic GABAA Receptors Modulate Thalamocortical Inputs in Layer 4 of Rat V1. Cerebral Cortex. 29(3):921-936.

***Product specific references for previous product # 845-GA4C which has been depleted and replaced with our product # 845A- GA4C which was produced by the same methods, using the same fusion protein antigen in new animals.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~64 kDa α4-subunit of the GABAA-R.

Click here to view our Western blotting and lysate preparation protocols.

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