Affinity purified from pooled serum. Learn more.

GABAA Receptor α4 Antibody

Catalog #: 845A-GA4C Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

*This antibody is a replacement for our original catalog # 845-GA4C. It was
produced by the same methods, using the same fusion protein antigen in
new animals.

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Mouse, Rat
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
GABRA4
Molecular Reference:
~64 kDa
Cite This Antibody:
PhosphoSolutions Cat# 845A-GA4C, RRID:AB_2800407
Antigen/Purification: ExpandCollapse

The antigen is a fusion protein from the cytoplasmic loop of the α4-subunit of rat GABAA receptor.

The antibody is prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl? channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six ?s, four ?s and four ?s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for ?- and ?-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a ?-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different ?-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Synonyms: ExpandCollapse

• GABA(A) receptor subunit alpha 4 antibody
• GABA(A) receptor subunit alpha-4 antibody
• GABR A4 antibody
• GABRA 4 antibody
• Gabra4 antibody
• Gamma aminobutyric acid (GABA) A receptor alpha 4 antibody
• Gamma aminobutyric acid A receptor alpha 4 antibody
• Gamma aminobutyric acid receptor alpha 4 subunit antibody
• Gamma aminobutyric acid receptor subunit alpha 4 antibody
• Gamma-aminobutyric acid receptor subunit alpha-4 antibody
• GBRA4_HUMAN antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References for previous cat. #845-GA4C*

* Our original antibody, cat. #845-GA4C has been depleted and replaced with our new version, cat. #845A-GA4C. It was produced by the same methods, using the same fusion protein antigen in new animals.

Wang, L., Kloc, M., Maher, E., Erisir, A. and Maffei, A., 2018. Presynaptic GABAA Receptors Modulate Thalamocortical Inputs in Layer 4 of Rat V1. Cerebral Cortex. PMID: 29373653

Marowsky A, Rudolph U, Fritschy JM, Arand M (2012) Tonic inhibition in principal cells of the amygdala: a central role for alpha-3 subunit-containing GABAA receptors. J Neurosci. 32(25):8611-9. PMID: 22723702

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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~64 kDa α4-subunit of the GABAA-R.

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