Affinity purified from pooled serum. Learn more.

GABAA Receptor α4 Antibody

Our rabbit polyclonal GABAA Receptor α4 total protein antibody is affinity purified from pooled serum. It is excellent for use in WB and IHC.

Catalog #: 845A-GA4C** Categories: , Datasheet:

$119.00$380.00

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**This antibody is a replacement for our original catalog #845-GA4C. It was produced by the same methods, using the same fusion protein antigen in new animals

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Applications:
WB 1:1000IHC 1:300 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
GABRA4
Molecular Weight:
~64 kDa
Cite This Antibody:
PhosphoSolutions Cat# 845A-GA4C, RRID:AB_2800407
Antigen/Purification: ExpandCollapse

The antigen is a fusion protein from the cytoplasmic loop of the α4-subunit of rat GABAA receptor.

The antibody is prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunitsresults in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Synonyms: ExpandCollapse

• GABA(A) receptor subunit alpha 4 antibody
• GABA(A) receptor subunit alpha-4 antibody
• GABR A4 antibody
• GABRA 4 antibody
• Gabra4 antibody
• Gamma aminobutyric acid (GABA) A receptor alpha 4 antibody
• Gamma aminobutyric acid A receptor alpha 4 antibody
• Gamma aminobutyric acid receptor alpha 4 subunit antibody
• Gamma aminobutyric acid receptor subunit alpha 4 antibody
• Gamma-aminobutyric acid receptor subunit alpha-4 antibody
• GBRA4_HUMAN antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Mouse | Rat


Immunohistochemistry: Mouse
PMID Dilution Publication
22723702*** not listed Marowsky, A., et al. 2012. Tonic inhibition in principal cells of the amygdala: a central role for alpha-3 subunit-containing GABAA receptors. J Neurosci. 32(25):8611-9.


Immunohistochemistry: Rat
PMID Dilution Publication
29373653*** 1:300 Wang, L., et al. 2019. . Presynaptic GABAA Receptors Modulate Thalamocortical Inputs in Layer 4 of Rat V1. Cerebral Cortex. 29(3):921-936.

***Product specific references for previous product # 845-GA4C which has been depleted and replaced with our product # 845A- GA4C which was produced by the same methods, using the same fusion protein antigen in new animals.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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