GABAA Receptor α3, N-Terminus Antibody

Catalog #: 824-GA3N Category: Datasheet:


  • SizePrice

Rabbit polyclonal antibody

Affinity purified
Species Tested:
Mouse, Rat
Species Reactivity:
Bovine, Canine, Human, Non-human primate, Zebrafish
WB 1:1000IHC 1:100
Gene Name:
Molecular Weight:
~51 kDa
Cite This Antibody:
PhosphoSolutions Cat# 824-GA3N, RRID:AB_2492102
Antigen/Purification: ExpandCollapse

The antigen is a synthetic peptide from N-terminal region of the α3-subunit of rat GABAA receptor.

The antibody is prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl-channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Synonyms: ExpandCollapse

• GABA A Receptor alpha 3 antibody
• GABA(A) receptor subunit alpha 3 antibody
• GABA(A) receptor subunit alpha-3 antibody
• GABR A3 antibody
• GABRA 3 antibody
• Gabra3 antibody
• Gamma aminobutyric acid (GABA) A receptor alpha 3 antibody
• Gamma aminobutyric acid A receptor alpha 3 antibody
• Gamma aminobutyric acid receptor subunit alpha 3 antibody
• Gamma-aminobutyric acid receptor subunit alpha-3 antibody
• GBRA3_HUMAN antibody
• MGC33793 antibody


100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols


Rat amygdala

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Mouse

Western blot: Mouse
PMID Dilution Publication
26446222*** Not listed Engin, E., et al. 2015. Tonic Inhibitory Control of Dentate Gyrus Granule Cells by α5-Containing GABAA Receptors Reduces Memory Interference. The Journal of Neuroscience, 35(40), 13698-13712.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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GABA A Receptor alpha 3 N-terminus Antibody
Western blot of mouse brain lysates from wild type (WT) and α3-knockout (K/O) animals showing specific immunolabeling of the ~51 kDa α3-subunit of the GABAA-R. The labeling was absent from a lysate prepared from α3-knockout animals.

Click here to view our Western blotting and lysate preparation protocols.
GABA A Receptor alpha 3 N-terminus Antibody
Immunostaining of rat amygdala showing labeling of GABAA α3 subunit (DAB, 1:100).

Download the protocol used for this image:

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