GABAA Receptor α3, N-Terminus Antibody

Catalog #: 824-GA3N Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Formulation:
Affinity purified
100% Homology:
Rat, Mouse
Applications:
WB 1:1,000IHC 1:100
Species:
Rabbit
Gene Name:
GABRA3
Molecular Reference:
~51 kDa
Cite This Antibody:
PhosphoSolutions Cat# 824-GA3N, RRID:AB_2492102
Antigen/Purification: ExpandCollapse

The antigen is a synthetic peptide from N-terminal region of the α3-subunit of rat GABAA receptor.

The antibody is prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl-channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Engin, E., Zarnowska, E. D., Benke, D., Tsvetkov, E., Sigal, M., Keist, R., Bolshakov, V.Y., Pearce, R.A., & Rudolph, U. (2015). Tonic Inhibitory Control of Dentate Gyrus Granule Cells by α5-Containing GABAA Receptors Reduces Memory Interference. The Journal of Neuroscience, 35(40), 13698-13712. PMID: 26446222

GABA A Receptor alpha 3 N-terminus Antibody
Western blot of mouse brain lysates from wild type (WT) and α3-knockout (K/O) animals showing specific immunolabeling of the ~51 kDa α3-subunit of the GABAA-R. The labeling was absent from a lysate prepared from α3-knockout animals.
GABA A Receptor alpha 3 N-terminus Antibody
Immunostaining of rat amygdala showing labeling of GABAA α3 subunit.

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