GABAA Receptor α1, N-Terminus Antibody

Catalog #: 812-GA1N Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Formulation:
Affinity purified
Species Reactivity:
Rat, Mouse, Bovine
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
GABRA1
Molecular Reference:
~51 kDa
Cite This Antibody:
PhosphoSolutions Cat# 812-GA1N, RRID:AB_2492100
Antigen/Purification: ExpandCollapse

The antigen is a peptide from the N-terminal region of the ?1-subunit of rat GABAA receptor, conjugated to keyhole limpet hemocyanin (KLH).

The antibody is prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl? channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six ?s, four ?s and four ?s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for ?- and ?-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a ?-subunit is required for benzo-diazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different ?- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Synonyms: ExpandCollapse

• ECA4 antibody
• EIEE19 antibody
• EJM antibody
• EJM5 antibody
• Gaba receptor alpha 1 polypeptide antibody
• GABA(A) receptor antibody
• GABA(A) receptor subunit alpha 1 antibody
• GABA(A) receptor subunit alpha-1 antibody
• GABA(A) receptor, alpha 1 antibody
• GABRA 1 antibody
• GABRA1 antibody
• Gamma aminobutyric acid (GABA) A receptor alpha 1 antibody
• Gamma aminobutyric acid A receptor alpha 1 antibody
• Gamma aminobutyric acid receptor subunit alpha 1 antibody
• Gamma aminobutyric acid type A receptor alpha1 subunit antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Engin, E., Zarnowska, E. D., Benke, D., Tsvetkov, E., Sigal, M., Keist, R., Bolshakov, V.Y., Pearce, R.A., & Rudolph, U. (2015). Tonic Inhibitory Control of Dentate Gyrus Granule Cells by ?5-Containing GABAA Receptors Reduces Memory Interference. The Journal of Neuroscience, 35(40), 13698-13712. PMID: 26446222

Wyatt, L. R., Finn, D. A., Khoja, S., Yardley, M. M., Asatryan, L., Alkana, R. L., & Davies, D. L. (2014). Contribution of P2X4 receptors to ethanol intake in male C57BL/6 mice. Neurochemical research, 39(6), 1127-1139. PMID: 24671605

Herman, M. A., & Roberto, M. (2014). Cell?type?specific tonic GABA signaling in the rat central amygdala is selectively altered by acute and chronic ethanol. Addiction biology. Aug. 29. PMID: 25170988

Mridula Rewal, Rachel Jurd, T. Michael Gill, Dao-Yao He, Dorit Ron, and Patricia H. Janak (2009) alpha-4-Containing GABAA Receptors in the Nucleus Accumbens Mediate Moderate Intake of Alcohol J. Neurosci., 29: 543-549. PMID: 19144854

Kurano, Y., Nakamura, M., Ichiba, M., Matsuda, M., Mizuno, E., Kato, M., Izumo, S. and Sano, A., 2006. Chorein deficiency leads to upregulation of gephyrin and GABAA receptor. Biochemical and biophysical research communications, 351(2), pp.438-442. PMID: 17070500

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GABA A Receptor alpha 1 N-terminus Antibody
Western blot of mouse forebrain lysates from Wild Type (WT) and ?1-knockout (K/O) animals showing specific immunolabeling of the ~51 kDa ?1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from ?1-knockout animals.

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