GABAA Receptor α1, N-Terminus Antibody

Catalog #: 812-GA1N Category: Datasheet:

$109.00$365.00

  • SizePrice
Clear

Rabbit polyclonal antibody

Formulation:
Affinity purified
Species Tested:
Mouse, Rat
Species Reactivity:
Bovine, Canine, Human, Non-human primate
Applications:
WB 1:1000IHC 1:100
Species:
Rabbit
Gene Name:
GABRA1
Molecular Weight:
~51 kDa
Cite This Antibody:
PhosphoSolutions Cat# 812-GA1N, RRID:AB_2492100
Antigen/Purification: ExpandCollapse

The antigen is a peptide from the N-terminal region of the α1-subunit of rat GABAA receptor, conjugated to keyhole limpet hemocyanin (KLH).

The antibody is prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzo-diazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Synonyms: ExpandCollapse

• ECA4 antibody
• EIEE19 antibody
• EJM antibody
• EJM5 antibody
• Gaba receptor alpha 1 polypeptide antibody
• GABA(A) receptor antibody
• GABA(A) receptor subunit alpha 1 antibody
• GABA(A) receptor subunit alpha-1 antibody
• GABA(A) receptor, alpha 1 antibody
• GABRA 1 antibody
• GABRA1 antibody
• Gamma aminobutyric acid (GABA) A receptor alpha 1 antibody
• Gamma aminobutyric acid A receptor alpha 1 antibody
• Gamma aminobutyric acid receptor subunit alpha 1 antibody
• Gamma aminobutyric acid type A receptor alpha1 subunit antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunohistochemistry

Rat Brain

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blotting: Mouse | Rat
Immunohistochemistry: Rat


Immunohistochemistry: Rat
PMID Dilution Publication
25170988 1:100 Herman, M., et al. 2016. Cell‐type‐specific tonic GABA signaling in the rat central amygdala is selectively altered by acute and chronic ethanol. Addiction biology. Jan;21(1):72-86.


Western Blotting: Mouse
PMID Dilution Publication
26446222 not listed Engin, E., et al. 2015. Tonic Inhibitory Control of Dentate Gyrus Granule Cells by α5-Containing GABAA Receptors Reduces Memory Interference. The Journal of Neuroscience, 35(40), 13698-13712.

24671605 1:1000 Wyatt, L.R., et al. 2014. Contribution of P2X4 receptors to ethanol intake in male C57BL/6 mice. Neurochemical research, 39(6), 1127-1139.

17070500 1:200 Kurano, Y., et al. 2006. . Chorein deficiency leads to upregulation of gephyrin and GABAA receptor. Biochemical and biophysical research communications, 351(2), pp.438-442..


Western Blotting: Rat
PMID Dilution Publication
19144854 1:500 Rewal, M., et al. 2009. α-Containing GABAA Receptors in the Nucleus Accumbens Mediate Moderate Intake of Alcohol. J. Neurosci., 29: 543 – 549.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

GABA A Receptor alpha 1 N-terminus Antibody
Western blot of mouse forebrain lysates from Wild Type (WT) and α1-knockout (K/O) animals showing specific immunolabeling of the ~51 kDa α1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from α1-knockout animals.

Click here to view our Western blotting and lysate preparation protocols.

You may also like…