Rabbit polyclonal antibody
- Formulation:
- Affinity purified
- 100% Homology:
- Rat, Mouse, Human, Bovine, Non-human primate, Canine
- Applications:
- WB 1:1,000
- Species:
- Rabbit
- Gene Name:
- GABRA1
- Molecular Reference:
- ~51 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# 811-GA1C, RRID:AB_2492099
Antigen/Purification: Collapse
The antigen is a fusion protein from the cytoplasmic loop of the α1-subunit of rat GABAA receptor.
The antibody is prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
Biological Significance: Collapse
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).
