GABAA Receptor α1 Antibody

Catalog #: 811-GA1C Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Formulation:
Affinity purified
100% Homology:
Rat, Mouse, Human, Bovine, Non-human primate, Canine
Applications:
WB 1:1,000
Species:
Rabbit
Gene Name:
GABRA1
Molecular Reference:
~51 kDa
Cite This Antibody:
PhosphoSolutions Cat# 811-GA1C, RRID:AB_2492099
Antigen/Purification: ExpandCollapse

The antigen is a fusion protein from the cytoplasmic loop of the α1-subunit of rat GABAA receptor.

The antibody is prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Wang, P., Eshaq, R. S., Meshul, C. K., Moore, C., Hood, R. L., & Leidenheimer, N. J. (2015). Neuronal gamma-aminobutyric acid (GABA) type A receptors undergo cognate ligand chaperoning in the endoplasmic reticulum by endogenous GABA. Frontiers in Cellular Neuroscience, 9, 188. PMID: 26041994

Herman, M. A., & Roberto, M. (2014). Cell‐type‐specific tonic GABA signaling in the rat central amygdala is selectively altered by acute and chronic ethanol. Addiction biology. Aug. 29. PMID: 25170988

Mridula Rewal, Rachel Jurd, T. Michael Gill, Dao-Yao He, Dorit Ron, and Patricia H. Janak (2009) alpha-4-Containing GABAA Receptors in the Nucleus Accumbens Mediate Moderate Intake of Alcohol J. Neurosci., 29: 543-549. PMID: 19144854

GABA A Receptor alpha 1 Antibody
Western blot of mouse forebrain lysates from wild type (WT) and α1-knockout (K/O) animals showing specific immunolabeling of the ~51 kDa α1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from α1-knockout animals.

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