GABAA Receptor α1 Antibody

Catalog #: 811-GA1C Category: Datasheet:
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$109.00 – $365.00

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Rabbit polyclonal antibody

Formulation:
Affinity purified
Species Tested:
Rat, Mouse
Species Reactivity:
Human, Bovine, Non-human primate, Canine
Applications:
WB 1:1000IHC 1:100
Species:
Rabbit
Gene Name:
GABRA1
Molecular Weight:
~51 kDa
Cite This Antibody:
PhosphoSolutions Cat# 811-GA1C, RRID:AB_2492099
Antigen/Purification: ExpandCollapse

The antigen is a fusion protein from the cytoplasmic loop of the ?1-subunit of rat GABAA receptor.

The antibody is prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Synonyms: ExpandCollapse

• ECA4 antibody
• EIEE19 antibody
• EJM antibody
• EJM5 antibody
• Gaba receptor alpha 1 polypeptide antibody
• GABA(A) receptor antibody
• GABA(A) receptor subunit alpha 1 antibody
• GABA(A) receptor subunit alpha-1 antibody
• GABA(A) receptor, alpha 1 antibody
• GABRA 1 antibody
• GABRA1 antibody
• Gamma aminobutyric acid (GABA) A receptor alpha 1 antibody
• Gamma aminobutyric acid A receptor alpha 1 antibody
• Gamma aminobutyric acid receptor subunit alpha 1 antibody
• Gamma aminobutyric acid type A receptor alpha1 subunit antibody
• Gamma-aminobutyric acid receptor subunit alpha-1 antibody
• GBRA1_HUMAN antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blotting: Rat
Immunohistochemistry: Rat

Immunohistochemistry: Rat
PMID Dilution Publication
25170988 1:100 Herman, M., et al. 2016. Cell‐type‐specific tonic GABA signaling in the rat central amygdala is selectively altered by acute and chronic ethanol. Addiction biology. Jan;21(1):72-86.

Western Blotting: Rat
PMID Dilution Publication
26041994 1:1000 Wang, P., et al. 2015. Neuronal gamma-aminobutyric acid (GABA) type A receptors undergo cognate ligand chaperoning in the endoplasmic reticulum by endogenous GABA. Frontiers in Cellular Neuroscience, 9, 188.
19144854 1:500 Rewal, M., et al. 2009. α-Containing GABAA Receptors in the Nucleus Accumbens Mediate Moderate Intake of Alcohol. J. Neurosci., 29: 543 – 549.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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GABA A Receptor alpha 1 Antibody
Western blot of mouse forebrain lysates from wild type (WT) and α1-knockout (K/O) animals showing specific immunolabeling of the ~51 kDa α1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from α1-knockout animals.

Click here to view our Western blotting and lysate preparation protocols.
Immunolabeling of mouse globus pallidus externus (subcortical structure) labelling the α1-subunit of the GABAA Receptor (cat # 811-GA1C, DAB, 1:100). Mice were transcardially perfused with 4% paraformaldehyde in PBS, then the brain was harvested and post fixed overnight. The harvested brains were stored in 20% sucrose/10% glycerol solution at 4C before sectioning. Frozen sections were cut to 35μM and stored in 1xPBS with 0.02% sodium azide until free-floating sections were labeled with above antibody. These images were kindly provided by Dr. Anton Reiner, Univ. of Tennessee Health Science Center (Memphis, TN).

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