FTO Antibody

Catalog #: 597-FTO Category: Datasheet:

$365.00

Mouse monoclonal antibody

Size:
100 µl
Formulation:
Protein G Purified
Specificity:
Rat, Mouse, Human
Applications:
WB 1:1,000IF 1:100
Species:
Mouse
Molecular Reference:
~58 kDa
Cite This Antibody:
PhosphoSolutions Cat# 597-FTO, RRID:AB_2492098
Antigen/Purification: ExpandCollapse

The antigen is synthetic peptide from the N-terminal region of rat FTO.

The antibody is Protein G purified culture supernatant.

Biological Significance: ExpandCollapse

The FTO gene is the most robust gene for common obesity characterized to date. FTO gene expression has been found to be significantly upregulated in the hypothalamus of rats after food deprivation and strongly negatively correlated with the expression of orexin peptide which is involved in the stimulation of food intake (Fredricksson R et al., 2008). Deletion analysis of FTO gene in mice showed that Fto is functionally involved in the control of both energy intake and energy expenditure (Fischer J et al., 2009).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for up to one year after date of receipt. Avoid freeze/thaw cycles.

Product Specific References

Zhou, J., Wan, J., Shu, X.E., Mao, Y., Liu, X.M., Yuan, X., Zhang, X., Hess, M.E., Brüning, J.C. and Qian, S.B., 2018. N6-methyladenosine guides mRNA alternative translation during integrated stress response. Molecular cell, 69(4), pp.636-647. PMID: 29429926

Wang, Y., Li, Y., Yue, M., Wang, J., Kumar, S., Wechsler-Reya, R.J., Zhang, Z., Ogawa, Y., Kellis, M., Duester, G. and Zhao, J.C., 2018. N 6-methyladenosine RNA modification regulates embryonic neural stem cell self-renewal through histone modifications. Nature Neuroscience, Feb 21(2):195-206. PMID: 29335608

Guan, B.J., van Hoef, V., Jobava, R., Elroy-Stein, O., Valasek, L.S., Cargnello, M., Gao, X.H., Krokowski, D., Merrick, W.C., Kimball, S.R. and Komar, A.A., 2017. A Unique ISR Program Determines Cellular Responses to Chronic Stress. Molecular Cell, 68(5), pp.885-900. PMID: 29220654

Yu, J., Chen, M., Huang, H., Zhu, J., Song, H., Zhu, J., Park, J. and Ji, S.J., 2017. Dynamic m6A modification regulates local translation of mRNA in axons. Nucleic Acids Research. Nov 23. PMID: 29186567

Zhou, J., Wan, J., Gao, X., Zhang, X., Jaffrey, S. R., & Qian, S. B. (2015). Dynamic m6A mRNA methylation directs translational control of heat shock response. Nature, 526(7574), 591-594. PMID: 26458103

Gulati, P., Avezov, E., Ma, M., Antrobus, R., Lehner, P., O’Rahilly, S., & Yeo, G. S. (2014). Fat mass and obesity-related (FTO) shuttles between the nucleus and cytoplasm. Bioscience reports, 34(5), 621-628. PMID: 25242086

Gulati P, Cheung MK, Antrobus R, Church CD, Harding HP, Tung YC, Rimmington D, Ma M, Ron D, Lehner PJ, Ashcroft FM, Cox RD, Coll AP, O’Rahilly S, Yeo GS (2013) Role for the obesity-related FTO gene in the cellular sensing of amino acids. Proc Natl Acad Sci U S A. Jan 28. PMID: 23359686

FTO Antibody
Western blot of rat testes lysate showing specific immunolabeling of the ~58 kDa Fto protein.

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