FTO Antibody

Catalog #: 597-FTO Categories: , Datasheet:

$109.00$365.00

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Mouse monoclonal antibody

Formulation:
Protein G Purified
Species Tested:
Human, Mouse, Rat
Expected Reactivity:
Bovine, Canine, Non-human primate, sheep
Applications:
WB 1:1000ICC 1:100-1:600 Don't see your application?
Species:
Mouse
Gene Name:
FTO
Molecular Weight:
~58 kDa
Cite This Antibody:
PhosphoSolutions Cat# 597-FTO, RRID:AB_2492098
Antigen/Purification: ExpandCollapse

The antigen is synthetic peptide from the N-terminal region of rat FTO.

The antibody is Protein G purified culture supernatant.

Biological Significance: ExpandCollapse

The FTO gene is the most robust gene for common obesity characterized to date. FTO gene expression has been found to be significantly upregulated in the hypothalamus of rats after food deprivation and strongly negatively correlated with the expression of orexin peptide which is involved in the stimulation of food intake (Fredricksson R et al., 2008). Deletion analysis of FTO gene in mice showed that FTO is functionally involved in the control of both energy intake and energy expenditure (Fischer J et al., 2009).

Synonyms: ExpandCollapse

• AlkB homolog 9 antibody
• ALKBH9 antibody
• Alpha-ketoglutarate-dependent dioxygenase FTO antibody
• AW743446 antibody
• Fat mass and obesity-associated protein antibody
• FATSO, MOUSE, HOMOLOG OF antibody
• Fto antibody
• FTO_HUMAN antibody
• GDFD antibody
• KIAA1752 antibody
• mKIAA1752 antibody
• Protein fatso antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for up to one year after date of receipt. Avoid freeze/thaw cycles.

Product Specific Protocols

Immunocytochemistry

Mouse Embryonic Fibroblasts (MEFs)

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Mouse

Western Blot: Human | Mouse


Immunocytochemistry: Mouse
PMID Dilution Publication
29186567 1:500 Yu, J., et al. 2018. Dynamic m6A modification regulates local translation of mRNA in axons. Nucleic Acids Research. Feb 16;46(3):1412-1423.
26458103 1:600 Zhour, J., et al. 2015. Dynamic m6A mRNA methylation directs translational control of heat shock response. Nature, 526(7574), 591-594.


Western Blot: Human
PMID Dilution Publication
30796352 not listed Yang, F., et al. 2019 Yang, F., Jin, H., Que, B., Chao, Y., Zhang, H., Ying, X., Zhou, Z., Yuan, Z., Su, J., Wu, B. and Zhang, W., 2019. Dynamic m 6 A mRNA methylation reveals the role of METTL3-m 6 A-CDCP1 signaling axis in chemical carcinogenesis. Oncogene, Jun;38(24):4755-4772.
30232453 not listed Choe, J., et al. 2018. mRNA circularization by METTL3–eIF3h enhances translation and promotes oncogenesis. Nature, 561(7724), p.556-560.


Western Blot: Mouse
PMID Dilution Publication
31436138 1:1000 Chokkalla, A.K. et al. 2019. . Transient Focal Ischemia Significantly Alters the m6A Epitranscriptomic Tagging of RNAs in the Brain. Stroke1, pp. STROKEAHA119026433.
31434789 1:1000 Zhang, Q., et al. 2019. The RNA demethylase FTO is required for maintenance of bone mass and functions to protect osteoblasts from genotoxic damage. Proceedings of the National Academy of Sciences, p.201905489.
30232453 not listed Choe,J., et al. 2018. mRNA circularization by METTL3–eIF3h enhances translation and promotes oncogenesis. Nature, 561(7724), p.556-560.
29429926 not listed Zhou, J. et al. 2018. N6-methyladenosine guides mRNA alternative translation during integrated stress response. Molecular cell, 69(4), pp.636-647.
29335608 1:1000 Wang., et al. 2018. N 6-methyladenosine RNA modification regulates embryonic neural stem cell self-renewal through histone modifications. Nature Neuroscience, Feb;21(2):195-206.
29220654 not listed Guan, B.J., et al. 2017. A Unique ISR Program Determines Cellular Responses to Chronic Stress. Molecular Cell, 68(5), pp.885-900.
26458103 1:1000 Zhou, J., et al. 2015. Dynamic m6A mRNA methylation directs translational control of heat shock response. Nature, 526(7574), 591-594.
25242086 not listed Gulati, P., et al. 2014. Fat mass and obesity-related (FTO) shuttles between the nucleus and cytoplasm. Bioscience reports, 34(5), 621-628.
23359686 not listed Gulati, P., et al. 2013. Role for the obesity-related FTO gene in the cellular sensing of amino acids. Proc Natl Acad Sci U S A. Feb 12;110(7):2557-62.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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FTO Antibody
Western blot of rat testes lysate showing specific immunolabeling of the ~58 kDa Fto protein.

Click here to view our Western blotting and lysate preparation protocols.

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