Fractin (fragment of actin) Antibody

Our rabbit polyclonal Fractin (fragment of actin) total protein antibody is neat serum. It is a marker of apoptosis and is excellent for use in WB and IHC.

Catalog #: 592-FRAC Categories: , Datasheet:

$109.00$325.00

  • SizePrice
Clear
Formulation:
Neat serum
Species Tested:
Human, Rat
Applications:
WB 1:1000IHC 1:50-1:1000ICC 1:100-1:500 Don't see your application?
Host Species:
Rabbit Polyclonal
Molecular Weight:
~32 kDa
Cite This Antibody:
PhosphoSolutions Cat# 592-FRAC, RRID:AB_2492097
Antigen/Purification: ExpandCollapse

The antigen is a Synthetic peptide taken from the C terminus of the ~32 kDa actin fragment.

The antibody is neat serum.

Biological Significance: ExpandCollapse

Fractin (fragment of actin) is a caspase-specific cleavage product of actin and serves as a novel marker of apoptosis-related events. The antibody has been shown to detect the processes and cell bodies of degenerating neurons and plaque-associated microglia in Alzheimer’s disease (Yang et al., 1998). It has recently been reported that Fractin may have a functional role in apoptotic signaling in oligodendrocytes (Schulz, R., et al., Glia, 2009, in press).

Storage

100 µl liquid. Contains no preservative. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunocytochemistry

rat.

Immunohistochemistry

Rat

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Human | Rat
Immunohistochemistry: Human | Rat


Immunocytochemistry: Human
PMID Dilution Publication
9466564 1:100 Yang, F., et al. 1998 Antibody to caspase-cleaved actin detects apoptosis in differentiated neuroblastoma and plaque-associated neurons and microglia in Alzheimer’s disease. The American Journal of Pathology, 152(2), p.379-389.


Immunocytochemistry: Rat
PMID Dilution Publication
11516428 1:400 Chen, T.A., et al. 2001 Inhibition of caspase-3-like activity reduces glutamate induced cell death in adult rat retina. Brain Research, 904(1), pp.177-188.
11431120 1:500 Adamec, E., et al. 2001 Multiple-label immunocytochemistry for the evaluation of nature of cell death in experimental models of neurodegeneration. Brain Research Protocols, 7(3), pp.193-202.


Immunohistochemistry: Human
PMID Dilution Publication
11841033 1:1000 Rossiter, J.P., et al. 2002 Caspase-3 activation and caspase-like proteolytic activity in human perinatal hypoxic-ischemic brain injury. Acta Neuropathologica, 103(1), pp.66-73.
10931367 1:50 Rossiter, J.P., et al. 2000 Caspase‐cleaved actin (fractin) immunolabelling of Hirano bodies. Neuropathology and Applied Neurobiology, 26(4), pp.342-346.
9466564 1:100 Yang, F., et al. 1998 Antibody to caspase-cleaved actin detects apoptosis in differentiated neuroblastoma and plaque-associated neurons and microglia in Alzheimer’s disease. The American Journal of Pathology, 152(2), p.379-389.


Immunohistochemistry: Rat
PMID Dilution Publication
11516428 1:400 Chen, T.A., et al. 2001 Inhibition of caspase-3-like activity reduces glutamate induced cell death in adult rat retina. Brain Research, 904(1), pp.177-188.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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