Anti-FOX3 (NeuN) Antibody (1B7)

4 Citations

Our Anti-FOX3 (NeuN) primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, human, mouse, and rat FOX3 (NeuN) and is Protein G purified. It is great for use in WB, IHC, ICC.

Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa FOX3 protein.
Immunofluorescence of a section of rat brain stem co-labeled with Anti-FOX3(cat. 583-FOX3, green, 1:1000) and Anti-MAP2 (cat. 1100-MAP2, red, 1:5000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.
Immunolabeling of cultured rat neurons showing strong nuclear and distal cytoplasmic labeling with anti-FOX3(cat. 583-FOX3, 1:1000, red). The complete absence of astrocyte staining is shown using anti-GFAP (cat. 621-GFAP, 1:1000, green) and nuclear staining was done with DAPI (blue).
Cell Line: human-derived iPSC cerebral organoids. Neurofilament-H (green), NeuN (purple), DAPI (turquoise). Image courtesy of Caroline Krall, Johns Hopkins University
Cell Line: human-derived iPSC cerebral organoids. MAP2 (pink), NeuN (yellow), DAPI (turquoise). Image courtesy of Caroline Krall, Johns Hopkins University
Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament-L (cat. 1453-NFL, 1:2000, green) and labeling of FOX3/NeuN (cat. 583-FOX3, 1:5000, red). The anti-NFL strongly labels the axons of basket calls and perikarya and processes of neuronal cells. The anti-FOX3/NeuN labels the nuclei and proximal cytoplasm of neurons. The blue is DAPI staining of nuclear DNA.
Immunofluorescence of a section of rat hippocampus showing specific labeling of UCHL1 (cat. 2060-UCHL1, 1:5000, green) in cell bodies and dendrites of neurons, and specific labeling of FOX3 (red). The blue is DAPI staining of nuclear DNA.
Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa FOX3 protein.
Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa FOX3 protein.
Immunofluorescence of a section of rat brain stem co-labeled with Anti-FOX3(cat. 583-FOX3, green, 1:1000) and Anti-MAP2 (cat. 1100-MAP2, red, 1:5000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.
Immunolabeling of cultured rat neurons showing strong nuclear and distal cytoplasmic labeling with anti-FOX3(cat. 583-FOX3, 1:1000, red). The complete absence of astrocyte staining is shown using anti-GFAP (cat. 621-GFAP, 1:1000, green) and nuclear staining was done with DAPI (blue).
Cell Line: human-derived iPSC cerebral organoids. Neurofilament-H (green), NeuN (purple), DAPI (turquoise). Image courtesy of Caroline Krall, Johns Hopkins University
Cell Line: human-derived iPSC cerebral organoids. MAP2 (pink), NeuN (yellow), DAPI (turquoise). Image courtesy of Caroline Krall, Johns Hopkins University
Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament-L (cat. 1453-NFL, 1:2000, green) and labeling of FOX3/NeuN (cat. 583-FOX3, 1:5000, red). The anti-NFL strongly labels the axons of basket calls and perikarya and processes of neuronal cells. The anti-FOX3/NeuN labels the nuclei and proximal cytoplasm of neurons. The blue is DAPI staining of nuclear DNA.
Immunofluorescence of a section of rat hippocampus showing specific labeling of UCHL1 (cat. 2060-UCHL1, 1:5000, green) in cell bodies and dendrites of neurons, and specific labeling of FOX3 (red). The blue is DAPI staining of nuclear DNA.
Bovine, Human, Mouse, Rat
ICC, IHC, WB
Mouse

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from PhosphoSolutions Datasheet
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SKU: 583-FOX3

Volume: 100 µL
Price:
$430

Product Specific References for Applications and Species

Immunocytochemistry: Mouse Hippocampal Neurons
PMID Dilution Publication
31956038 not listed Zhu, Z., et al. 2020. Zika Virus Targets Glioblastoma Stem Cells through a SOX2-Integrin αvβ5 Axis. Cell Stem Cell, 26(2):187-204.
27277805 1:200 Kolisnyk, B., et al. 2016. Cholinergic Regulation of hnRNPA2/B1 Translation by M1 Muscarinic Receptors. The Journal of Neuroscience, 36(23), 6287-6296.
Immunohistochemistry: Mouse Hippocampus
PMID Dilution Publication
27312991 1:500 Kolisnyk, B., et al. 2017. Cholinergic surveillance over hippocampal RNA metabolism and Alzheimer's-like pathology. Cerebral Cortex, 27(7), pp.3553-3567.
Immunohistochemistry: Rat
PMID Dilution Publication
32351379 1:200 Wang, J., et al. 2020. A New Rat Model of Chronic Cerebral Hypoperfusion Resulting in Early-Stage Vascular Cognitive Impairment. Frontiers in Aging Neuroscience, 12, p.86.

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