FOX3 (NeuN) Antibody

Catalog #: 583-FOX3 Categories: , , Datasheet:

$109.00$365.00

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Robust marker of neurons and neuronal stem cells!

 

Formulation:
Protein G Purified
Species Tested:
Bovine, Human, Mouse, Rat,
Applications:
WB 1:2000IHC 1:500-1:1000ICC 1:200-1:1000
Species:
Mouse monoclonal
Gene Name:
RBFOX3
Molecular Reference:
~46/48 kDa
Cite This Antibody:
PhosphoSolutions Cat# 583-FOX3, RRID:AB_2492096
Antigen/Purification: ExpandCollapse

The antigen is a recombinant protein taken from the N-terminus of human FOX3 expressed in and purified from E. Coli.

The antibody is Protein G purified from culture supernatant.

Biological Significance: ExpandCollapse

FOX3, also known as NeuN and hexaribonucleotide binding protein 3, is a neuron-specific RNA binding nuclear protein involved in the regulation of pre-mRNA alternative splicing (Kim et al., 2009). FOX3 dependent alternative splicing of Numb has recently been shown to play an important role in the progression of neuronal differentiation during vertebrate development (Kim et al., 2013).

Synonyms: ExpandCollapse

• FLJ56884 antibody
• FLJ58356 antibody
• Fox-1 homolog C antibody
• fox1 homolog C antibody
• Fox 3 antibody
• FOX3NeuN antibody
• hexaribonucleotide binding protein 3 antibody
• HRNBP3 antibody
• NeuN antibody
• NEUN antibody
• neuronal nuclei antibody
• Rbfox3 antibody
• RFOX3_HUMAN antibody
• RNA binding protein fox-1 homolog 3 antibody
• RNA binding protein, fox 1 homolog (C. elegans) 3 antibody

Storage

100 µl in PBS + 50% glycerol + 5 mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunocytochemistry

Cultured rat neurons

Immunohistochemistry

Rat Brain Stem

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Mouse Hippocampal Neurons

Immunohistochemistry: Mouse


Immunocytochemistry: Mouse Hippocampal Neurons
PMID Dilution Publication
27277805 1:200 Kolisnyk, B., et al. 2016. Cholinergic Regulation of hnRNPA2/B1 Translation by M1 Muscarinic Receptors. The Journal of Neuroscience, 36(23), 6287-6296.


Immunohistochemistry: Mouse Hippocampus
PMID Dilution Publication
27312991 1:500 Kolisnyk, B., et al. 2017. Cholinergic Surveillance over Hippocampal RNA Metabolism and Alzheimer’s-Like Pathology. Cerebral Cortex, bhw177.

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FOX3 (NeuN) Antibody
Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa FOX3 protein.

Click here to view our Western blotting and lysate preparation protocols.
Immunolabeling of cultured rat neurons showing strong nuclear and distal cytoplasmic labeling with anti-FOX3(cat # 583-FOX3, 1:1000, red). The complete absence of astrocyte staining is shown using anti-GFAP (cat #621-GFAP, 1:1000, green) and nuclear staining was done with DAPI (blue).

Download the protocol used for this image:
Immunofluorescence of a section of rat brain stem showing colabeled with Anti-FOX3(cat. # 583-FOX3, green, 1:1000) and Anti-MAP2 (cat #1100-MAP2, red, 1:5000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.

Download the protocol used for this image:

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