This product is produced by our partner Aves Labs. Learn more.

FOX3 (NeuN) Antibody

Chicken Polyclonal Antibody

Catalog #: NUN Categories: , , , Datasheet:

$399.00

Size:
1000 µl
Formulation:
Affinity Purified IgY
Species Tested:
Mouse
Expected Reactivity:
Human, Rat
Applications:
IHC 1:1000-1:2000ICC 1:1000-1:2000
Host Species:
Chicken Polyclonal
Gene Name:
RBFOX3
Molecular Weight:
46, 48 kDa
Cite This Antibody:
Aves Labs Cat# NUN, RRID:AB_2313556
Antigen/Purification: ExpandCollapse

Hens were immunized with a synthetic peptide-keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to the Fox-3 gene product (also known as the Neu-N antigen), but was shared between the human NP_001076044, NCBI) and mouse (NP_001020102, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 µg/mL, and the preparation filter-sterilized.

Synonyms: ExpandCollapse
  • RNA binding protein fox-1 homolog 3
  • Fox-1 homolog C
  • Neuronal nuclei antigen
  • NeuN antigen

Storage

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS,” pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Store at 4˚C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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