Affinity purified from pooled serum. Learn more.

Fragile X Mental Retardation Protein (FMRP) (Ser499) Antibody

We are the original manufacturer of our Fragile X Mental Retardation Protein (FMRP) (Ser499) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. Optimized in WB.

Catalog #: p1125-499 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Chicken, Human, Mouse, Rat
Expected Reactivity:
Bovine, Canine, Non-human primate, Sheep, Xenopus, Zebra fish
Applications:
WB 1:1000IHC 1:250-1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
FMR1
Molecular Weight:
~71 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1125-499, RRID:AB_2492094
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser499 of rat FMRP.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Fragile X Mental Retardation Protein (FMRP) is an RNA-binding protein that plays an essential role in cognitive brain function. Mutations in the FMR1 gene, which codes for FMRP, can result in fragile X syndrome, autism, as well as other cognitive deficits (Brown et al.,1998, Goodlin-Jones et al., 2004). Phosphorylation of the highly conserved Ser499 has been shown to trigger hierarchical phosphorylation of nearby serines and may play a role in suppressing target mRNA translation (Ceman et al., 2003, Narayanan et al. 2008).

Synonyms: ExpandCollapse

• FMR 1 antibody
• Fmr1 antibody
• Fmr1 gene antibody
• FMR1_HUMAN antibody
• FMRP antibody
• Fragile X mental retardation 1 antibody
• Fragile X mental retardation 1 protein antibody
• Fragile X mental retardation protein 1 antibody
• Fragile X mental retardation protein antibody
• fragile X mental retardation syndrome-related protein 1 antibody
• fragile X mental retardation, autosomal homolog 1 antibody
• FRAXA antibody
• fxr1 antibody
• MGC87458 antibody
• POF antibody
• POF1 antibody
• Protein FMR-1 antibody
• Protein FMR1 antibody
• wu:fb16f11 antibody
• wu:fd18c10 antibody
• zgc:66226 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunohistochemistry

Mouse Retina

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Chicken | Human | Mouse
Western Blot: Chicken | Human | Mouse | Rat


Immunohistochemistry: Chicken
PMID Dilution Publication
26719241 1:1000 Guimarães-Souza, E.M., et al. 2016 Fragile X Mental Retardation Protein expression in the retina is regulated by light. Experimental Eye Research, 146, pp.72-82.


Immunohistochemistry: Human
PMID Dilution Publication
30630951 1:250-1:1000 Chen, Y., et al. 2019 Aggregation of the nucleic acid–binding protein TDP-43 occurs via distinct routes that are coordinated with stress granule formation. Journal of Biological Chemistry, 294(10), pp.3696-3706.


Immunohistochemistry: Mouse
PMID Dilution Publication
26719241 1:1000 Guimarães-Souza, E.M., et al. 2016 Fragile X Mental Retardation Protein expression in the retina is regulated by light. Experimental Eye Research, 146, pp.72-82.


Western Blot: Chicken
PMID Dilution Publication
32449186 not listed Yu, X., et al. 2020 Dynamics of the Fragile X Mental Retardation Protein Correlates with Cellular and Synaptic Properties in Primary Auditory Neurons following Afferent Deprivation. Journal of Comparative Neurology.
26719241 1:2000 Guimarães-Souza, E.M., et al. 2016 Fragile X Mental Retardation Protein expression in the retina is regulated by light. Experimental Eye Research, 146, pp.72-82.


Western Blot: Human
PMID Dilution Publication
30630951 1:1000 Chen, Y., et al. 2019 Aggregation of the nucleic acid–binding protein TDP-43 occurs via distinct routes that are coordinated with stress granule formation. Journal of Biological Chemistry, 294(10), pp.3696-3706.


Western Blot: Mouse
PMID Dilution Publication
31112584 1:1000 DeMarco, B., et al. 2019 FMRP-G-quadruplex mRNA-miR-125a interactions: Implications for miR-125a mediated translation regulation of PSD-95 mRNA. PloS One, 14(5), p.e0217275.
27957526 1:1000 Bartley, C.M., et al. 2016 Mammalian FMRP S499 is phosphorylated by CK2 and promotes secondary phosphorylation of FMRP. eNeuro, 3(6).
26719241 1:2000 Guimarães-Souza, E.M., et al. 2016 Fragile X Mental Retardation Protein expression in the retina is regulated by light. Experimental Eye Research, 146, pp.72-82.
26580204 1:500 Reynolds, C.D., et al. 2015 FMRP S499 is phosphorylated independent of mTORC1-S6K1 activity. PLoS One, 9(5), p.e96956.
24806451 not listed Bartley, C.M., et al. 2014 FMRP S499 is phosphorylated independent of mTORC1-S6K1 activity. PLoS One, 9(5), p.e96956.


Western Blot: Rat
PMID Dilution Publication
23831253 not listed Bernard, P.B., et al. 2013 Phosphorylation of FMRP and alterations of FMRP complex underlie enhanced mLTD in adult rats triggered by early life seizures. Neurobiology of Disease, 59, pp.1-17.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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