Fibrillarin Antibody

Our mouse monoclonal Fibrillarin protein antibody is total IgG fraction. It is great for staining nucleoli and is excellent for use in WB, IHC, and ICC.

Catalog #: 560-FIB Categories: , , , Datasheet:

$109.00$325.00

  • SizePrice
Clear
Formulation:
Total IgG fraction
Species Tested:
Bovine, Chicken, Drosophila, Human, Mouse, Pig, Rat
Expected Reactivity:
Most Mammals
Applications:
WB 1:1000IHC 1:100-1:500ICC 1:100-1:500 Don't see your application?
Host Species:
Mouse monoclonal
Gene Name:
NOP1
Molecular Weight:
~34 kDa
Cite This Antibody:
PhosphoSolutions Cat# 560-FIB, RRID:AB_2492093
Antigen/Purification: ExpandCollapse

The antigen is a yeast nuclear preparations.

The antibody is total IgG fraction.

Biological Significance: ExpandCollapse

Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archaea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3). This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).

Synonyms: ExpandCollapse

• 34 kD nucleolar scleroderma antigen antibody
• 34 kDa nucleolar scleroderma antigen antibody
• Fbl antibody
• FBRL_HUMAN antibody
• FIB antibody
• FIB1 antibody
• FLRN antibody
• Histone-glutamine methyltransferase antibody
• Nop1p antibody
• RNA U3 small nucleolar interacting protein 1 antibody
• RNU3IP1 antibody
• rRNA 2′ O methyltransferase fibrillarin antibody
• rRNA 2′-O-methyltransferase fibrillarin antibody

Storage

100 µl in PBS + 50% glycerol + 5 mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunocytochemistry

Human neuroblastoma SH-SY5Y cells
HeLa Cells

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Paeschke, K., Simonsson, T., Postberg, J., Rhodes, D. and Lipps, H.J., 2005. Telomere end-binding proteins control the formation of G-quadruplex DNA structures in vivo. Nature Structural & Molecular Biology, 12(10), p.847. PMID: 16142245

Vermaak, D., Henikoff, S. and Malik, H.S., 2005. Positive selection drives the evolution of rhino, a member of the heterochromatin protein 1 family in Drosophila. PLoS Genetics, 1(1), 96-108. PMID: 16103923

Tyagi, S. and Alsmadi, O., 2004. Imaging native β-actin mRNA in motile fibroblasts. Biophysical Journal, 87(6), pp.4153-4162. PMID: 15377515

Newton, K., Petfalski, E., Tollervey, D. and Cáceres, J.F., 2003. Fibrillarin is essential for early development and required for accumulation of an intron-encoded small nucleolar RNA in the mouse. Molecular and Cellular Biology, 23(23), pp.8519-8527. PMID: 14612397

Aris, J.P. and Blobel, G., 1988. Identification and characterization of a yeast nucleolar protein that is similar to a rat liver nucleolar protein. The Journal of Cell Biology, 107(1), pp.17-31. PMID: 3292539

Ochs, R.L., Lischwe, M.A., Spohn, W.H. and Busch, H., 1985. Fibrillarin: a new protein of the nucleolus identified by autoimmune sera. Biology of the Cell, 54(2), pp.123-133. PMID: 2933102

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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