EWS Antibody

$109.00$365.00

  • SizePrice
Clear

Mouse monoclonal antibody

Formulation:
Protein G Purified
Specificity:
Human
Applications:
WB 1:1000IHC 1:500
Species:
Mouse Monoclonal
Molecular Reference:
~85 kDa
Cite This Antibody:
PhosphoSolutions Cat# 540-EWS, RRID:AB_2650521
Antigen/Purification: ExpandCollapse

The antigen is recombinant full length human EWS expressed in and purified from E. Coli.

The antibody is Protein G purified from culture supernatant.

Biological Significance: ExpandCollapse

The Ewing Sarcoma Breakpoint Region 1 (EWSR1) gene encodes the EWS protein, which along with FUS and TAF15 comprises the FET family of RNA-binding proteins.  EWS is primarily expressed in the nucleus, but has also been found in the cytoplasm of secretory cell types (Andersson et al., 2008).  Defects of EWSR1 are known to cause Ewing sarcoma and have been implicated in amyotrophic lateral sclerosis (ALS) (Svetoni et al., 2016).  Recently, EWS has been shown to directly interact with oncogenic ETS transcription factors in the promotion of prostate cancer (Kedage et al., 2016).

Storage

100 µl in PBS + 5 mM sodium azide.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least 1 year. Avoid freeze/thaw cycles.

General References:

Andersson MK, Ståhlberg A, Arvidsson Y, Olofsson A, Semb H, Stenman G, Nilsson O, Aman P (2008). The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response. BMC Cell Biol. 11:9:37.

Svetoni F, Frisone P, Paronetto MP (2016) Role of FET proteins in neurodegenerative disorders. RNA Biol. 2016 Nov;13(11):1089-1102

Kedage V, Selvaraj N, Nicholas TR, Budka JA, Plotnik JP, Jerde TJ, Hollenhorst PC (2016) An Interaction with Ewing’s Sarcoma Breakpoint Protein EWS Defines a Specific Oncogenic Mechanism of ETS Factors Rearranged in Prostate Cancer. Cell Rep. 2016 Oct 25;17(5):1289-1301.

EWSR1 antibody
Western blot of HeLa cell lysate showing specific immunolabeling of the ~85 kDa EWS protein.

You may also like…