EWS Antibody

Catalog #: 540-EWS Categories: , , , Datasheet:

$109.00$365.00

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Mouse monoclonal antibody

Formulation:
Protein G Purified
Species Tested:
Canine, Human, Mouse, Rat
Applications:
WB 1:1000IHC 1:1000ICC 1:1000
Species:
Mouse Monoclonal
Gene Name:
EWSR1
Molecular Reference:
~85 kDa
Cite This Antibody:
PhosphoSolutions Cat# 540-EWS, RRID:AB_2650521
Antigen/Purification: ExpandCollapse

The antigen is recombinant full length human EWS expressed in and purified from E. Coli.

The antibody is Protein G purified from culture supernatant.

Biological Significance: ExpandCollapse

The Ewing Sarcoma Breakpoint Region 1 (EWSR1) gene encodes the EWS protein, which along with FUS and TAF15 comprises the FET family of RNA-binding proteins.  EWS is primarily expressed in the nucleus, but has also been found in the cytoplasm of secretory cell types (Andersson et al., 2008).  Defects of EWSR1 are known to cause Ewing sarcoma and have been implicated in amyotrophic lateral sclerosis (ALS) (Svetoni et al., 2016).  Recently, EWS has been shown to directly interact with oncogenic ETS transcription factors in the promotion of prostate cancer (Kedage et al., 2016).

Synonyms: ExpandCollapse
  • 4 antibody
  • 4 Ewing sarcoma breakpoint region 1 protein antibody
  • Ewing sarcoma breakpoint region 1 antibody
  • Ewing sarcoma breakpoint region 1 protein antibody
  • Ewings sarcoma EWS Fli1 type 1 oncogene antibody
  • EWS antibody
  • EWS oncogene antibody
  • EWS RNA binding protein 1 antibody
  • EWS_HUMAN antibody
  • EWSR 1 antibody
  • Ewsr1 antibody
  • EWSR1 protein antibody
  • RNA binding protein EWS antibody
  • RNA-binding protein EWS antibody

Storage

100 µl in PBS + 50% glycerol + 5 mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunocytochemistry

HeLa Cells

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References:

Andersson MK, Ståhlberg A, Arvidsson Y, Olofsson A, Semb H, Stenman G, Nilsson O, Aman P (2008). The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response. BMC Cell Biol. 11:9:37.

Svetoni F, Frisone P, Paronetto MP (2016) Role of FET proteins in neurodegenerative disorders. RNA Biol. 2016 Nov;13(11):1089-1102

Kedage V, Selvaraj N, Nicholas TR, Budka JA, Plotnik JP, Jerde TJ, Hollenhorst PC (2016) An Interaction with Ewing’s Sarcoma Breakpoint Protein EWS Defines a Specific Oncogenic Mechanism of ETS Factors Rearranged in Prostate Cancer. Cell Rep. 2016 Oct 25;17(5):1289-1301.

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EWSR1 antibody
Western blot of HeLa cell lysate showing specific immunolabeling of the ~85 kDa EWS protein.

Click here to view our Western blotting and lysate preparation protocols.
Immunofluorescence of HeLa cells showing clear localized labeling of the DNA in the nucleus with Anti-EWS (cat. # 540-EWS, 1:1000, gre¬¬en) while Anti-Vimentin (cat #2105-VIM, 1:5000, red) labels the cytoplasmic intermediate filament, and Hoechst DNA stain (blue).

Download the protocol used for this image:

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