Affinity purified from pooled serum. Learn more.

Eukaryotic Initiation Factor 4A2 (eIF4A2)

Catalog #: 495-eIF4A2 Category: Datasheet:

$109.00$365.00

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Rabbit Polyclonal Antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Bovine, Human, Mouse, Non-human primate, Rat
Applications:
WB 1:1000
Species:
Rabbit Polyclonal
Molecular Reference:
~ 46 kDa
Cite This Antibody:
PhosphoSolutions Cat# 495-eIF4A2, RRID:AB_2630324
Antigen/Purification: ExpandCollapse

The antigen is a peptide from the N-terminal region of the human eIF4A2 protein.

The antibody is prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

 

Biological Significance: ExpandCollapse

The eIF4A, eukaryotic initiation factor 4A, are a set of proteins, eIF4A1, eIF4A2, and eIF4A3, that belong to the extensive DEAD-box RNA helicase family (Weinstein et al., 1997). They are involved in many aspects of RNA metabolism by virtue of their RNA-binding capacity and ATPase activity (Lu et al., 2014).  eIF4A2 is a highly conserved gene for one of the protein-synthesis initiation factors involved in the binding of mRNA to the ribosome (Gingras et al., 1999). In humans eIF4A2 is expressed in all normal tissues, though highly expressed in skeletal muscle (Sudo et al., 1995).  Studies suggest eIF4A2 may play a role in some cancers, specifically breast and lung cancer, along with melanoma (Shaoyan et al., 2013).

Synonyms: ExpandCollapse
  • ATP dependent RNA helicase eIF4A 2 antibody
  • ATP-dependent RNA helicase eIF4A-2 antibody
  • BM-010 antibody
  • DDX2B antibody
  • eIF 4A II antibody
  • eIF-4A-II antibody
  • EIF4A antibody
  • eIF4A II antibody
  • eIF4A-II antibody
  • EIF4A2 antibody
  • EIF4F antibody
  • Eukaryotic initiation factor 4A II antibody
  • Eukaryotic initiation factor 4A-II antibody
  • eukaryotic translation initiation factor 4A isoform 2 antibody
  • Eukaryotic translation initiation factor 4A2 antibody
  • IF4A2_HUMAN antibody
  • N-terminally processed antibod

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

General References

Lu, W. T., Wilczynska, A., Smith, E., & Bushell, M. (2014). The diverse roles of the eIF4A family: you are the company you keep. Biochemical Society Transactions, 42(1), 166-172.

Shaoyan X, Juanjuan Y, Yalan T, Ping H, Jianzhong L, Qinian W (2013) Downregulation of eIF4A2 in Non-Small-Cell Lung Cancer Associates with Poor Prognosis. Clin Lung Cancer, 14(6):658-65.

Gingras AC, Raught B, Sonenberg N (1999) eIF4 initiation factors: Effectors of mRNA Recruitment to Ribosomes and Regulators of Translation. Annu Rev Biochem, 68: 913-63.

Weinstein DC, Honore E, Hemmati-Brivanlou A (1997) Epidermal Induction and Inhibition of Neural Fate by Translation Initiation Factor 4AIII. Development 124: 4235-4242.

Sudo K, Takahashi E, Nakamura Y (1995) Isolation and Mapping of the Human eIF4A2 Gene Homologous to the Murine Protein Synthesis Initiation Factor 4A-II gene Eif4a2. Cytogenet Cell Genet, 71: 385-8.

 

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eIF4A2 antibody
Western blot of mouse testes lysate showing specific immunolabeling of the ~46 kDa eukaryotic initiation factor 4A2, eIF4A2, protein.

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