Affinity purified from pooled serum. Learn more.

ERK/MAPK Antibody

Catalog #: 500-ERK Categories: , Datasheet:

$109.00$365.00

  • SizePrice
Clear

Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Rat
Applications:
WB 1:1000ICC 1:250 Don't see your application?
Species:
Rabbit
Gene Name:
MAPK1/2
Molecular Weight:
~42 kDa - 44 kDa
Cite This Antibody:
PhosphoSolutions Cat# 500-ERK, RRID:AB_2492089
Antigen/Purification: ExpandCollapse

The antigen is a peptide corresponding to amino acid residues from the C-terminus of rat ERK/MAPK.

The antibody is prepared from pooled rabbit serum by affinity purification.

Biological Significance: ExpandCollapse

Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002; Ahn, 1993; Tanoue and Nishida, 2003; Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).

Synonyms: ExpandCollapse
  • ERK 1 antibody
  • ERK 2 antibody
  • ERK-2 antibody
  • ERK1 antibody
  • erk1/2 antibody
  • ERK2 antibody
  • ERT1 antibody
  • ERT2 antibody
  • Extracellular signal regulated kinase 1 antibody
  • Extracellular signal-regulated kinase 2 antibody
  • MAP kinase 1 antibody
  • MAP kinase 2 antibody
  • MAP kinase isoform p42 antibody
  • MAP kinase isoform p44 antibody
  • MAPK 1 antibody
  • MAPK 2 antibody
  • MAPK 3 antibody
  • Mapk1 antibody
  • MAPK2 antibody
  • MAPK3 antibody
  • Mitogen-activated protein kinase 1 antibody
  • Mitogen-activated protein kinase 2 antibody
  • MK01_HUMAN antibody
  • p38 antibody
  • p40 antibody
  • p41 antibody
  • p42-MAPK antibody
  • PRKM 2 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunocytochemistry

Human lymphocytes (blood)

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Human
Western Blot: Human


Immunocytochemistry: Human
PMID Dilution Publication
27743527 1:250 Erickson, C.A., et al. 2017 Initial analysis of peripheral lymphocytic extracellular signal related kinase activation in autism. Journal of Psychiatric Research, 84, pp.153-160.


Western Blot: Human
PMID Dilution Publication
27743527 1:250 Erickson, C.A., et al. 2017 Initial analysis of peripheral lymphocytic extracellular signal related kinase activation in autism. Journal of Psychiatric Research, 84, pp.153-160.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

ERK/MAPK Antibody
Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~42-44 kDa ERK/MAPK protein.

Click here to view our Western blotting and lysate preparation protocols.

You may also like…