Affinity purified from pooled serum. Learn more.

ERK/MAPK (Thr202, Tyr204) Antibody

We are the original manufacturer of our ERK/MAPK (Thr202, Tyr204) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. Optimized in WB and IHC.

Catalog #: p160-2024 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Rat
Expected Reactivity:
Bovine, Canine, Chicken, Non-human primate, Xenopus, Zebra fish
Applications:
WB 1:1000IHC 1:100-1:500ICC 1:250 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
MAPK1/2
Molecular Weight:
~42 - 44 kDa
Cite This Antibody:
PhosphoSolutions Cat# p160-2024, RRID:AB_2492090
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr202 and Tyr204 of rat ERK/MAPK.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002; Ahn, 1993; Tanoue and Nishida, 2003; Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).

Synonyms: ExpandCollapse
  • ERK 1 antibody
  • ERK 2 antibody
  • ERK-2 antibody
  • ERK1 antibody
  • erk1/2 antibody
  • ERK2 antibody
  • ERT1 antibody
  • ERT2 antibody
  • Extracellular signal regulated kinase 1 antibody
  • Extracellular signal-regulated kinase 2 antibody
  • MAP kinase 1 antibody
  • MAP kinase 2 antibody
  • MAP kinase isoform p42 antibody
  • MAP kinase isoform p44 antibody
  • MAPK 1 antibody
  • MAPK 2 antibody
  • MAPK 3 antibody
  • Mapk1 antibody
  • MAPK2 antibody
  • MAPK3 antibody
  • Mitogen-activated protein kinase 1 antibody
  • Mitogen-activated protein kinase 2 antibody
  • MK01_HUMAN antibody
  • p38 antibody
  • p40 antibody
  • p41 antibody
  • p42-MAPK antibody
  • PRKM 2 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunohistochemistry

Mouse dentate gyrus.

Immunocytochemistry

Human Lymphocytes (blood).

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Human
Immunohistochemistry: Human
Western Blot: Human | Mouse


Immunocytochemistry: Human
PMIDDilutionPublication
277435271:250 Erickson, C.A., et al. 2019 Initial analysis of peripheral lymphocytic extracellular signal related kinase activation in autism. Journal of Psychiatric Research, 84, pp.153-160.


Immunohistochemistry: Human
PMIDDilutionPublication
227652201:100 Iwanga, R., et al. 2012 Expression of Six1 in luminal breast cancers predicts poor prognosis and promotes increases in tumor initiating cells by activation of extracellular signal-regulated kinase and transforming growth factor-beta signaling pathways. Breast Cancer Research, 14(4), p.R100.


Western Blot: Human
PMIDDilutionPublication
277435271:250 Erickson, C.A., et al. 2017 Initial analysis of peripheral lymphocytic extracellular signal related kinase activation in autism. Journal of Psychiatric Research, 84, pp.153-160.
26181363not listed Morgan, J.E., et al. 2015 The class II transactivator (CIITA) is regulated by post-translational modification cross-talk between ERK1/2 phosphorylation, mono-ubiquitination and Lys63 ubiquitination. Bioscience Reports, 35(4).


Western Blot: Mouse
PMIDDilutionPublication
292421671:100 Rainville, J.R., et al. 2019 Membrane-initiated nuclear trafficking of the glucocorticoid receptor in hypothalamic neurons. Steroids/i1,142:55-64.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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