Affinity purified from pooled serum. Learn more.

ERK/MAPK (Thr202, Tyr204) Antibody

Catalog #: p160-2024 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse, Human, Xenopus, Bovine, Chicken, Non-human primate, Zebra fish, Canine
Applications:
WB 1:1000IHC 1:500
Species:
Rabbit
Gene Name:
MAPK1/2
Molecular Weight:
~42 - 44 kDa
Cite This Antibody:
PhosphoSolutions Cat# p160-2024, RRID:AB_2492090
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr202 and Tyr204 of rat ERK/MAPK.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002; Ahn, 1993; Tanoue and Nishida, 2003; Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).

Synonyms: ExpandCollapse
  • ERK 1 antibody
  • ERK 2 antibody
  • ERK-2 antibody
  • ERK1 antibody
  • erk1/2 antibody
  • ERK2 antibody
  • ERT1 antibody
  • ERT2 antibody
  • Extracellular signal regulated kinase 1 antibody
  • Extracellular signal-regulated kinase 2 antibody
  • MAP kinase 1 antibody
  • MAP kinase 2 antibody
  • MAP kinase isoform p42 antibody
  • MAP kinase isoform p44 antibody
  • MAPK 1 antibody
  • MAPK 2 antibody
  • MAPK 3 antibody
  • Mapk1 antibody
  • MAPK2 antibody
  • MAPK3 antibody
  • Mitogen-activated protein kinase 1 antibody
  • Mitogen-activated protein kinase 2 antibody
  • MK01_HUMAN antibody
  • p38 antibody
  • p40 antibody
  • p41 antibody
  • p42-MAPK antibody
  • PRKM 2 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Rainville, J.R., Weiss, G.L., Evanson, N., Herman, J.P., Vasudevan, N. and Tasker, J.G., 2017. Membrane-Initiated Nuclear Trafficking of the Glucocorticoid Receptor in Hypothalamic Neurons. Steroids. Dec 12. pii: S0039-128X(17)30231-3. PMID: 29242167

Erickson, C.A., Ray, B., Wink, L.K., Bayon, B.L., Pedapati, E.V., Shaffer, R., Schaefer, T.L. and Lahiri, D.K., 2016. Initial analysis of peripheral lymphocytic extracellular signal related kinase activation in autism. Journal of Psychiatric Research. Jan;84:153-160. PMID: 27743527

Morgan, J. E., Shanderson, R. L., Boyd, N. H., Cacan, E., & Greer, S. F. (2015). The class II transactivator (CIITA) is regulated by post-translational modification cross-talk between ERK1/2 phosphorylation, mono-ubiquitination and Lys63 ubiquitination. Bioscience reports, 35(4), e00233. PMID: 26181363

Shuto, T., Kuroiwa, M., Koga, Y., Kawahara, Y., Sotogaku, N., Toyomasu, K. and Nishi, A., 2013. Acute effects of resveratrol to enhance cocaine-induced dopamine neurotransmission in the striatum. Neuroscience letters, 542, pp.107-112. PMID: 23499958

Iwanaga, R., Wang, C.A., Micalizzi, D.S., Harrell, J.C., Jedlicka, P., Sartorius, C.A., Kabos, P., Farabaugh, S.M., Bradford, A.P. and Ford, H.L., 2012. Expression of Six1 in luminal breast cancers predicts poor prognosis and promotes increases in tumor initiating cells by activation of extracellular signal-regulated kinase and transforming growth factor-beta signaling pathways. Breast Cancer Research, 14(4), p.R100. PMID: 22765220

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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ERK/MAPK thr202, tyr204 Antibody
Western blot of human T47D cell lysate showing specific immunolabeling of ~42-44 kDa ERK/MAPK protein phosphorylated at Thr202/Tyr204 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
ERK/MAPK thr202, tyr204 Antibody
Immunostaining of granule cells in the dentate gyrus of saline treated mouse showing ERK/MAPK when phosphorylated at Thr202/Tyr204 (red) and nuclei (blue). Photo courtesy of Robert Wine.

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