Affinity purified from pooled serum. Learn more.

EphrinB (Tyr331) Antibody

Catalog #: p1110-331 Categories: , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Expected Reactivity:
Bovine, Chicken, Human, Xenopus, Zebra fish
Applications:
WB 1:1000 Don't see your application?
Species:
Rabbit
Gene Name:
EPHB2
Molecular Weight:
~46 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1110-331, RRID:AB_2492088
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr331 of chicken EphrinB. Note: Chicken Tyr331 is the homolog of mouse and rat Tyr343, human Tyr344 and also Xenopus Tyr324.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vanderhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work demonstrated that Tyr331 of EphrinB was phosphorylated in HEK293 cells after stimulation by the soluble EphB2 receptor tyrosine kinase (Kalo et al., 2001).

Synonyms: ExpandCollapse
  • CEK5 ligand antibody
  • EFNB1 antibody
  • ELK L antibody
  • ELK ligand antibody
  • ELKL antibody
  • EPH related receptor tyrosine kinase ligand 2 antibody
  • Ephrin B1 antibody
  • Ephrin B2 antibody
  • LERK2 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Bong, Y.S., Park, Y.H., Lee, H.S., Mood, K., Ishimura, A. and Daar, I.O., 2004. Tyr-298 in ephrinB1 is critical for an interaction with the Grb4 adaptor protein. Biochemical Journal, 377(2), pp.499-507. PMID: 14535844

Dufour, A., Seibt, J., Passante, L., Depaepe, V., Ciossek, T., Frisén, J., Kullander, K., Flanagan, J.G., Polleux, F. and Vanderhaeghen, P., 2003. Area specificity and topography of thalamocortical projections are controlled by ephrin/Eph genes. Neuron, 39(3), pp.453-465. PMID: 12895420

Flanagan, J.G. and Vanderhaeghen, P., 1998. The ephrins and Eph receptors in neural development. Annual Review of Neuroscience, 21(1), pp.309-345. PMID: 9530499

Oike, Y., Ito, Y., Hamada, K., Zhang, X.Q., Miyata, K., Arai, F., Inada, T., Araki, K., Nakagata, N., Takeya, M. and Kisanuki, Y.Y., 2002. Regulation of vasculogenesis and angiogenesis by EphB/ephrin-B2 signaling between endothelial cells and surrounding mesenchymal cells. Blood, 100(4), pp.1326-1333. PMID: 12149214

Palmer, A., Zimmer, M., Erdmann, K.S., Eulenburg, V., Porthin, A., Heumann, R., Deutsch, U. and Klein, R., 2002. EphrinB phosphorylation and reverse signaling: regulation by Src kinases and PTP-BL phosphatase. Molecular Cell, 9(4), pp.725-737. PMID: 11983165

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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EphrinB tyr331 Antibody
Western blot of rat testes lysate showing specific immunolabeling of the ~46 kDa EphrinB phosphorylated at Tyr331 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 min).

Click here to view our Western blotting and lysate preparation protocols.

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