Rabbit polyclonal antibodyPooled Serum
- 100 µl
- Affinity Purified from Pooled Serum
- Rat, Mouse, Human, Non-human primate, Zebra fish, Canine
- WB 1:1,000
- Molecular Reference:
- ~46 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p1110-298, RRID:AB_2492086
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho Tyr298 of Xenopus EphrinB. Note: Xenopus Tyr298 is the homolog of human, mouse and rat Tyr317 and also chicken Tyr305.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Biological Significance: ExpandCollapse
EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vancerhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cellto cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling (Kalo, et al., 2001).