Affinity purified from pooled serum. Learn more.

EphrinB (Tyr298) Antibody

Catalog #: p1110-298 Category: Datasheet:

$365.00

Rabbit polyclonal antibody

Pooled Serum
Size:
100 µl
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Rat, Mouse, Human, Non-human primate, Zebra fish, Canine
Applications:
WB 1:1,000
Species:
Rabbit
Molecular Reference:
~46 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1110-298, RRID:AB_2492086
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho Tyr298 of Xenopus EphrinB. Note: Xenopus Tyr298 is the homolog of human, mouse and rat Tyr317 and also chicken Tyr305.

The antibody is  prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vancerhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cellto cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling (Kalo, et al., 2001).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Bong, Y.S., Park, Y.H., Lee, H.S., Mood, K., Ishimura, A. and Daar, I.O. Tyr-298 in ephrinB1 is critical for an interaction with the Grb4 adaptor protein, Biochem. J. 377:499-507 (2004).

Dufour, A., Seibt, J., Passante, L., Depaepe, V., Ciossek, T., Frisen, J., Kullander, K., Flanagan, J.G., Polleux, F. and Vanderhaeghen, P. Area specificity and topography of thalamocortical projections are controlled by ephrin/Eph genes, Neuron 39:453-465 (2003).

Flanagan, J.G. and Vanderhaeghen, P. The ephrins and Eph receptors in neural development, Annu. Rev. Neurosci. 21:309-345 (1998).

Flanagan, J.G. and Vanderhaeghen, P. The ephrins and Eph receptors in neural development, Annu. Rev. Neurosci. 21:309-345 (1998).

Oike, Y., Ito, Y., Hamada, K., Zhang, X.Q., Miyata, K., Arai, F., Inada, T., Araki, K., Nakagata, N., Takeya, M., Kisanuki, Y.Y., Yanagisawa, M., Gale, N.W. and Suda, T, Regulation of vasculogenesis and angiogenesis by EphB/ephrin-B2 signaling between endothelial cells and surrounding mesenchymal cells, Blood 100:1326-1333 (2002).

Palmer, A., Zimmer, M., Erdmann, K.S., Eulenburg, V., Porthin, A., Heumann, R., Deutsch, U. and Klein, R Ephrin B phosphorylation and reverse signaling: regulation by Src kinases and PTP-BL Phosphatase, Mol Cell 9:725-737 (2002)

EphrinB tyr298 Antibody
Western blot of rat testes lysate showing specific immunolabeling of the ~46 kDa EphrinB phosphorylated at Tyr298 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

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