Affinity purified from pooled serum. Learn more.

Elk-1 (Ser383) Antibody

Catalog #: p155-383 Category: Datasheet:

$109.00$365.00

  • SizePrice
Clear

Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
100% Homology:
Rat, Mouse, Human, Non-human primate, Zebra fish, Canine
Applications:
WB 1:1,000
Species:
Rabbit
Gene Name:
ELK1
Molecular Reference:
~46 kDa
Cite This Antibody:
PhosphoSolutions Cat# p155-383, RRID:AB_2492085
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser383 of human Elk-1.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Elk-1 is a transcription factor involved in mediating gene transcription in response to growth factors (Hill and Treisman, 1995). Elk-1 is thought to be phosphorylated by MAP kinase at Ser383 and phosphorylation at this site is essential for the transcriptional activity of Elk-1 (Li et al., 2003). Phosphorylation of Elk-1 has also been implicated in synaptic plasticity in the adult hippocampus (Thiels et al., 2002).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Hill CS, Treisman R (1995) Transcriptional regulation by extracellular signals: mechanisms and specificity. Cell 80:199-211.

Li QJ, Yang SH, Maeda Y, Sladek FM, Sharrocks AD. Martins-Green M (2003) MAP kinase phosphorylationdependent activation of Elk-1 leads to activation of the co-activator p300. EMBO J 22:281-291.

Thiels E, Kanterewicz BI, Norman ED, Trzaskos, JM, Klann E (2002) Long-term depression in the adult hippocampus in vivo involves activation of extracellular signal-regulated kinase and phosphorylation of Elk-1. J Neurosci 22:2054-2062.

Elk-1 ser383 Antibody
Western blot of recombinant Elk-1 showing specific immunolabeling of the ~46 kDa Elk-1 phosphorylated at Ser383 in the right lane (-). Phosphospecificity is shown in the left lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

You may also like…