Affinity purified from pooled serum. Learn more.

Elk-1 (Ser383) Antibody

We are the original manufacturer of our Elk-1 (Ser383) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. Optimized in WB.

Catalog #: p155-383 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Expected Reactivity:
Canine, Human, Non-human primate, Zebra fish
Applications:
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
ELK1
Molecular Weight:
~46 kDa
Cite This Antibody:
PhosphoSolutions Cat# p155-383, RRID:AB_2492085
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser383 of human Elk-1.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Elk-1 is a transcription factor involved in mediating gene transcription in response to growth factors (Hill and Treisman, 1995). Elk-1 is thought to be phosphorylated by MAP kinase at Ser383 and phosphorylation at this site is essential for the transcriptional activity of Elk-1 (Li et al., 2003). Phosphorylation of Elk-1 has also been implicated in synaptic plasticity in the adult hippocampus (Thiels et al., 2002).

Synonyms: ExpandCollapse
  • ELK 1 antibody
  • Elk1 antibody
  • ELK1 member of ETS oncogene family antibody
  • ELK1 protein antibody
  • ELK1, ETS transcription factor antibody
  • ELK1_HUMAN antibody
  • ELK2 member of ETS oncogene family antibody
  • ETS domain containing protein Elk 1 antibody
  • ETS domain containing protein Elk1 antibody
  • ETS domain protein Elk1 antibody
  • ETS domain-containing protein Elk-1 antibody
  • ETS like gene 1 antibody
  • Member of ETS oncogene family antibody
  • Oncogene Elk1 antibody
  • Tyrosine kinase (ELK1) oncogene antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Hill, C.S. and Treisman, R., 1995. Transcriptional regulation by extracellular signals: mechanisms and specificity. Cell, 80(2), pp.199-211. PMID: 8521730

Li, Q.J., Yang, S.H., Maeda, Y., Sladek, F.M., Sharrocks, A.D. and Martins‐Green, M., 2003. MAP kinase phosphorylation‐dependent activation of Elk‐1 leads to activation of the co‐activator p300. The EMBO Journal, 22(2), pp.281-291. PMID: 8521730

Thiels, E., Kanterewicz, B.I., Norman, E.D., Trzaskos, J.M. and Klann, E., 2002. Long-term depression in the adult hippocampus in vivo involves activation of extracellular signal-regulated kinase and phosphorylation of Elk-1. Journal of Neuroscience, 22(6), pp.2054-2062. PMID: 8521730

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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