Affinity purified from pooled serum. Learn more.

eEF1A2 (Ser358) Antibody

Catalog #: p153-358 Category: Datasheet:


  • SizePrice

Rabbit polyclonal antibody

Pooled Serum
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse, Human, Xenopus, Chicken, Non-human primate, Sheep
WB 1:1000
Gene Name:
Molecular Weight:
~50 kDa
Cite This Antibody:
PhosphoSolutions Cat# p153-358, RRID:AB_2492084
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser358 of human eEF1A2.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Eukaryotic Elongation Factor eEF1A exists in two variant forms, eEF1A1 and eEF1A2. While eEF1A1 is almost ubiquitously expressed in humans, eEF1A2 is predominantly found in heart, brain, and skeletal muscle (Knudsen et al., 1993). Expression of eEF1A2 may have a role in ovarian cancer, as its expression is drastically increased in human ovarian tumors (Anand et al., 2002). Due to differences in structural models between the two isoforms, eEF1A1 and eEF1A2 likely have variant-specific phosphorylation sites (Soares et al., 2009). Ribosome-associated JNK phosphorylates Ser358 on eEF1A2 to promote degradation of newly synthesized polypeptides by the proteasome (Gandin et al., 2013).

Synonyms: ExpandCollapse
  • eEF1A-2 antibody
  • Eef1a2 antibody
  • EEF1AL antibody
  • EF 1 alpha 2 antibody
  • EF-1-alpha-2 antibody
  • EF1A antibody
  • EF1A2_HUMAN antibody
  • Elongation factor 1 A 2 antibody
  • Elongation factor 1 alpha antibody
  • Elongation factor 1 alpha 2 antibody
  • Elongation factor 1-alpha 2 antibody
  • Eukaryotic elongation factor 1 A-2 antibody
  • Eukaryotic translation elongation factor 1 alpha 2 antibody
  • FLJ41696 antibody
  • HS1 antibody
  • OTTHUMP00000031776 antibody
  • Statin like antibody
  • Statin S1 antibody
  • Statin-S1 antibody
  • STN antibody
  • STNL antibody


100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10- mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Gandin, V., Gutierrez, G. J., Brill, L. M., Varsano, T., Feng, Y., Aza-Blanc, P., Au, Q., McLaughlan, S., Ferreira, T.A., Alain, T., Sonenberg, N., Topisirovic I, & Ronai ZA (2013). Degradation of newly synthesized polypeptides by ribosome-associated RACK1/c-Jun N-terminal kinase/eukaryotic elongation factor 1A2 complex. Molecular and cellular biology, 33(13), 2510-2526. PMID: 23608534



  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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eEF1A2 Ser358 Antibody
Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~50 kDa eEF1A2 protein phosphorylated at Ser358 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 min).

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