Affinity purified from pooled serum. Learn more.

Dynamin (Ser778) Antibody

We are the original manufacturer of our Dynamin (Ser778) sheep polyclonal phosphospecific antibody, affinity purified from pooled serum. Optimized in WB.

Catalog #: p1050-778 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Expected Reactivity:
Bovine, Canine, Chicken, Human
Applications:
WB 1:1000 Don't see your application?
Host Species:
Sheep Polyclonal
Gene Name:
DNM1
Molecular Weight:
~95 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1050-778, RRID:AB_2492083
Antigen/Purification: ExpandCollapse

The antigen is a  phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser778 of rat dynamin.

The antibody is prepared from pooled sheep serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.

Synonyms: ExpandCollapse
  • B dynamin antibody
  • D100 antibody
  • DNM 1 antibody
  • DNM antibody
  • DNM1 antibody
  • DYN1_HUMAN antibody
  • Dynamin antibody
  • Dynamin-1 antibody
  • Dynamin1 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Mouse


Western Blot: Mouse
PMID Dilution Publication
28052246 not listed Vargas, K.J., et al. 2017 Synucleins have multiple effects on presynaptic architecture. Cell Reports, 18(1), pp.161-173.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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