Affinity purified from pooled serum. Learn more.

Dynamin (Ser774) Antibody

Catalog #: p1050-774 Category: Datasheet:

$109.00$365.00

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Sheep polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse, Human, Bovine, Chicken, Non-human primate, Canine
Applications:
WB 1:1000IF 1:1000IHC 1:10
Species:
Sheep
Gene Name:
DNM1
Molecular Weight:
~95 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1050-774, RRID:AB_2492082
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser774 of rat dynamin.

The antibody is prepared from pooled sheep serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of Ser774 by GSK3 has recently been shown to control activity-dependent bulk endocytosis of synaptic vesicles (Clayton et al., 2010).

Synonyms: ExpandCollapse
  • B dynamin antibody
  • D100 antibody
  • DNM 1 antibody
  • DNM antibody
  • DNM1 antibody
  • DYN1_HUMAN antibody
  • Dynamin antibody
  • Dynamin-1 antibody
  • Dynamin1 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Vargas, K.J., Schrod, N., Davis, T., Fernandez-Busnadiego, R., Taguchi, Y.V., Laugks, U., Lucic, V. and Chandra, S.S., 2017. Synucleins Have Multiple Effects on Presynaptic Architecture. Cell Reports, 18(1), pp.161-173.  PMID: 28052246

Sergio Leal-Ortiz, Clarissa L. Waites, Ryan Terry-Lorenzo, Pedro Zamorano, Eckart D. Gundelfinger, and Craig C. Garner (2008) Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis. J. Cell Biol., 181: 831 – 846. PMID: 18519737

Tan TC, Valova VA, Malladi CS, Graham ME, Berven LA, Jupp OJ, Hansra G, McClure SJ, Sarcevic B, Boadle RA, Larsen MR, Cousin MA, Robinson PJ (2003) cdk5 is essential for synaptic vesicle endocytosis. Nat Cell Biol 5:701-710.  PMID: 12855954

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Dynamin ser774 Antibody
Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95 kDa dynamin phosphorylated at Ser774 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

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