Affinity purified from pooled serum. Learn more.

Dynamin (Ser774) Antibody

We are the original manufacturer of our Dynamin (Ser774) sheep polyclonal phospho antibody, affinity purified from pooled serum. Optimized in WB, IF, and IHC.

Catalog #: p1050-774 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Rat, Mouse
Expected Reactivity:
Bovine, Canine, Chicken, Human, Non-human primate
Applications:
WB 1:1000ICC 1:1000 Don't see your application?
Host Species:
Sheep Polyclonal
Gene Name:
DNM1
Molecular Weight:
~95 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1050-774, RRID:AB_2492082
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser774 of rat dynamin.

The antibody is prepared from pooled sheep serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of Ser774 by GSK3 has recently been shown to control activity-dependent bulk endocytosis of synaptic vesicles (Clayton et al., 2010).

Synonyms: ExpandCollapse
  • B dynamin antibody
  • D100 antibody
  • DNM 1 antibody
  • DNM antibody
  • DNM1 antibody
  • DYN1_HUMAN antibody
  • Dynamin antibody
  • Dynamin-1 antibody
  • Dynamin1 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunocytochemistry

Rat B104 Neuroblastoma cells

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Western Blot: Mouse | Rat


Immunocytochemistry: Rat
PMID Dilution Publication
12855954 not listed Tan, T.C., et al. 2003 Cdk5 is essential for synaptic vesicle endocytosis. Nature Cell Biology, 5(8), p.701.


Western Blot: Mouse
PMID Dilution Publication
28052246 not listed Vargas, K.J., et al. 2017 Synucleins have multiple effects on presynaptic architecture. Cell Reports, 18(1), pp.161-173.


Western Blot: Rat
PMID Dilution Publication
18519737 not listed Leal-Ortiz, S., et al. 2008 Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis. The Journal of Cell Biology, 181(5), pp.831-846.
12855954 not listed Tan, T.C., et al. 2003 Cdk5 is essential for synaptic vesicle endocytosis. Nature Cell Biology, 5(8), p.701.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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