Anti-Doublecortin Antibody (3E1 )

Name: Anti-Doublecortin Antibody
Brand: PhosphoSolutions
SKU: 451-DCX
Price: 430.0 USD
Availability: InStock

Our Anti-Doublecortin primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, human, mouse, and rat Doublecortin and is Protein G purified. It is great for use in WB, IHC, ICC.

Immunofluorescence of rat cortical neuron-glial cells showing strong cytoplasmic labeling of a small population of developing neurons and their processes with Anti-DCX (cat. 451-DCX, 1:1000, red) while Anti-MAP2 (cat. 1100-MAP2, 1:10,000, green) labels dendrites and perikarya or mature neurons, and additional nuclear staining was done with DAPI (blue). Anti-doublecortin is an excellent marker of early developing neuronal cells.

Western blot of postnatal day 3 rat brain lysate showing specific immunolabeling of the ~35 kDa and ~45 kDa doublecortin protein.

Immunofluorescence of cultured rat neurons showing strong cytoplasmic staining of doublecortin (cat. 451-DCX, 1:1000,green) in developing neurons and GFAP (cat. 621-GFAP, 1:1000, red)

Immunofluorescence of rat cortical neuron-glial cells showing strong cytoplasmic labeling of a small population of developing neurons and their processes with Anti-DCX (cat. 451-DCX, 1:1000, red) while Anti-MAP2 (cat. 1100-MAP2, 1:10,000, green) labels dendrites and perikarya or mature neurons, and additional nuclear staining was done with DAPI (blue). Anti-doublecortin is an excellent marker of early developing neuronal cells.

Species Reactivity

Bovine, Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

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from PhosphoSolutions Datasheet

Trial Size

SKU: 451-DCX

Volume: 100 µL

Variant

Price:

$430

Quantity:

Product Details

Target

Doublecortin

Target Description

Doublecortin, or DCX, is a microtubule associated protein that is expressed almost exclusively in very early neuronal development (Brown et al., 2003), making it an excellent marker for developing neuronal cells. Defects in the DCX gene lead to X-linked lissencephaly which is characterized by a lack of normal folds on the surface of the brain resulting in a smooth cerebral cortex caused by abnormal migration of neurons during development (des Portes et al., 1998; Gleeson et al., 1998).

Format

Protein G Purified

Clonality

Monoclonal

Clone

3E1

Isotype

IgG2a

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

DCX

Molecular Weight

35, 45 kDa

Antigen

Full length recombinant human protein expressed in and purified from E. coli.

Species Reactivity

Bovine, Human, Mouse, Rat

Antibody Registry ID

AB_2492079

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Protein G purified culture supernatant.

Buffer

PBS + 50% glycerol and 5 mm NaN3

Dilution Ranges

WB: 1:5000
WB Brain: 1:5000
IHC: 1:1000
ICC: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~35 kDa & ~45 kDa doublecortin protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

DBCN antibody, Dbct antibody, DC antibody, DCX antibody, DCX_HUMAN antibody, Doublecortex antibody, Doublin antibody, FLJ51296 antibody, Lis X antibody, Lis-X antibody, Lissencephalin X antibody, Lissencephalin-X antibody, Lissencephaly X linked antibody, Lissencephaly X linked doublecortin antibody, LISX antibody, Neuronal migration protein doublecortin antibody, OTTHUMP00000023859 antibody, OTTHUMP00000023860 antibody, OTTHUMP00000216315 antibody, OTTHUMP00000216316 antibody, SCLH antibody, XLIS antibody

UniProt Details

UniProt (Human): O43602
UniProt (Immunogen Species): O43602

NCBI Gene ID

1641

Bulk Order Anti-Doublecortin Antibody

Product Specific Protocols

Western Blot Protocol: Download

General References

Brown, J.P., Couillard‐Després, S., Cooper‐Kuhn, C.M., Winkler, J., Aigner, L. and Kuhn, H.G., 2003. Transient expression of doublecortin during adult neurogenesis. Journal of Comparative Neurology, 467(1), pp.1-10. PMID: 14574675
des Portes, V., Pinard, J.M., Billuart, P., Vinet, M.C., Koulakoff, A., Carrié, A., Gelot, A., Dupuis, E., Motte, J., Berwald-Netter, Y. and Catala, M., 1998. A novel CNS gene required for neuronal migration and involved in X-linked subcortical laminar heterotopia and lissencephaly syndrome. Cell, 92(1), pp.51-61. PMID: 9489699
Gleeson, J.G., Allen, K.M., Fox, J.W., Lamperti, E.D., Berkovic, S., Scheffer, I., Cooper, E.C., Dobyns, W.B., Minnerath, S.R., Ross, M.E. and Walsh, C.A., 1998. Doublecortin, a brain-specific gene mutated in human X-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein. Cell, 92(1), pp.63-72.PMID: 9489700

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Anti-Doublecortin Antibody (3E1 )

Our Anti-Doublecortin primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, human, mouse, and rat Doublecortin and is Protein G purified. It is great for use in WB, IHC, ICC.

SKU: 451-DCX

Product Details

Bulk Order Anti-Doublecortin Antibody

Product Specific Protocols

General References

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