Doublecortin (DCX) Antibody

Catalog #: 451-DCX Categories: , , , Datasheet:

$109.00$365.00

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Excellent marker for neuronal stem cells!

 

Formulation:
Protein G Purified
Species Tested:
Rat, Mouse, Human, Bovine
Expected Reactivity:
Most mammals
Applications:
WB 1:5000IHC 1:1000ICC 1:1000
Species:
Mouse monoclonal
Gene Name:
DCX
Molecular Weight:
~35 kDa and ~45 kDa
Cite This Antibody:
PhosphoSolutions Cat# 451-DCX, RRID:AB_2492079
Antigen/Purification: ExpandCollapse

The antigen is a full length recombinant human doublecortin expressed in and purified from E. coli.

The antibody is Protein G purified from culture supernatant.

Biological Significance: ExpandCollapse

Doublecortin, or DCX, is a microtubule associated protein that is expressed almost exclusively in very early neuronal development (Brown et al., 2003), making it an excellent marker for developing neuronal cells. Defects in the DCX gene lead to X-linked lissencephaly which is characterized by a lack of normal folds on the surface of the brain resulting in a smooth cerebral cortex caused by abnormal migration of neurons during development (des Portes et al., 1998; Gleeson et al., 1998).

Synonyms: ExpandCollapse
  • DBCN antibody
  • Dbct antibody
  • DC antibody
  • DCX antibody
  • DCX_HUMAN antibody
  • Doublecortex antibody
  • Doublin antibody
  • FLJ51296 antibody
  • Lis X antibody
  • Lis-X antibody
  • Lissencephalin X antibody
  • Lissencephalin-X antibody
  • Lissencephaly X linked antibody
  • Lissencephaly X linked doublecortin antibody
  • LISX antibody
  • Neuronal migration protein doublecortin antibody
  • OTTHUMP00000023859 antibody
  • OTTHUMP00000023860 antibody
  • OTTHUMP00000216315 antibody
  • OTTHUMP00000216316 antibody
  • SCLH antibody
  • XLIS antibody

Storage

100 µl in PBS + 50% glycerol + 5 mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunocytochemistry

Rat neuron-glial cells

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Brown JP, Couillard-Després S, Cooper-Kuhn CM, Winkler J, Aigner L, Kuhn HG (2003) Transient expression of doublecortin during adult neurogenesis. J Comp Neurol. Dec 1;467(1):1-10.

des Portes V, Pinard JM, Billuart P, Vinet MC, Koulakoff A, Carrié A, Gelot A, Dupuis E, Motte J, Berwald-Netter Y, Catala M, Kahn A, Beldjord C and Chelly J. (1998) A novel CNS gene required for neuronal migration and involved in X-linked subcortical laminar heterotopia and lissencephaly syndrome. Cell 92:51-61.

Gleeson JG, Allen KM, Fox JW, Lamperti ED, Berkovic S, Scheffer I, Cooper EC, Dobyns WB, Minnerath SR, Ross ME and Walsh CA. (1998) Doublecortin, a brain-specific gene mutated in human X-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein. Cell 92:63-72.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Doublecortin (DCX) Antibody
Western blot of postnatal day 3 rat brain lysate showing specific immunolabeling of the ~35 kDa and ~45 kDa doublecortin protein.

Click here to view our Western blotting and lysate preparation protocols.

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