Affinity purified from pooled serum. Learn more.

Dopamine Transporter (DAT) (Thr53) Antibody

Catalog #: p435-53 Category: Datasheet:


  • SizePrice

Rabbit polyclonal antibody

Pooled Serum
Affinity Purified from Pooled Serum
Species Reactivity:
WB 1:1000
Gene Name:
Molecular Reference:
~55 kDa
Cite This Antibody:
PhosphoSolutions Cat# p435-53, RRID:AB_2492078
Antigen/Purification: ExpandCollapse

The antigen is a  phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr53 of rat DAT.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.


Biological Significance: ExpandCollapse

The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996). It has been shown that phosphorylation at Thr53 directly affects dopamine influx and amphetamine-stimulated substrate efflux, indicating that the Thr53 residue plays a major role in transport activity (Foster et al., 2012).

Synonyms: ExpandCollapse
  • DA transporter antibody
  • DAT 1 antibody
  • DAT antibody
  • DAT1 antibody
  • Dopamine transporter 1 antibody
  • Dopamine transporter antibody
  • PKDYS antibody
  • SC6A3_HUMAN antibody
  • SLC6A3 antibody
  • Sodium dependent dopamine transporter antibody
  • Sodium-dependent dopamine transporter antibody
  • Solute carrier family 6 (neurotransmitter transporter dopamine), member 3 antibody
  • Solute carrier family 6 (neurotransmitter transporter), member 3 antibody
  • Solute carrier family 6 member 3 antibody
  • Variable number tandem repeat (VNTR) antibody


100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Brodnik, Z.D., Alonso, I.P., Xu, W., Zhang, Y., Kortagere, S. and España, R.A., 2018. Hypocretin receptor 1 involvement in cocaine-associated behavior: Therapeutic potential and novel mechanistic insights. Brain research, S0006-8993(18)30410-4. PMID: 30071195

Gowrishankar, R., Gresch, P.J., Davis, G.L., Katamish, R.M., Riele, J.R., Stewart, A.M., Vaughan, R.A., Hahn, M.K. and Blakely, R.D., 2018. Region-Specific Regulation of Presynaptic Dopamine Homeostasis by D2 Autoreceptors Shapes the In Vivo Impact of the Neuropsychiatric Disease-Associated DAT Variant Val559. J Neurosci., 38(23):5302-5312. PMID: 29739866

Lameth, J., Gervais, A., Colin, C., Lévêque, P., Jay, T.M., Edeline, J.M. and Mallat, M., 2017. Acute neuroinflammation promotes cell responses to 1800 MHz GSM electromagnetic fields in the rat cerebral cortex. Neurotoxicity research, 32(3), pp.444-459. PMID: 28578480

Aher, Y.D., Subramaniyan, S., Shanmugasundaram, B., Sase, A., Saroja, S.R., Holy, M., Höger, H., Beryozkina, T., Sitte, H.H., Leban, J.J. and Lubec, G., 2016. A novel heterocyclic compound CE-104 enhances spatial working memory in the radial arm maze in rats and modulates the dopaminergic system. Front Behav Neurosci., 10:20. PMID: 26941626

Sase, A., Aher, Y., Saroja, S. R., Ganesan, M. K., Sase, S., Holy, M., Hoger, H., Bakulev, V., Ecker, G., Langer, T., Sitte, H.H., Leban, J., & Lubec, G. (2015). A heterocyclic compound CE-103 inhibits dopamine reuptake and modulates dopamine transporter and dopamine D1-D3 containing receptor complexes. Neuropharmacology. Sept 22 pii: S0028-3908(15)300046-0. PMID: 26407764

James D. Foster, Jae-Won Yang, Amy E. Moritz, Sathyavathi ChallaSivaKanaka, Margaret A. Smith, Marion Holy, Kyle Wilebski, Harald H. Sitte, and Roxanne A. Vaughan (2012) Dopamine Transporter Phosphorylation Site Threonine 53 Regulates Substrate Reuptake and Amphetamine-stimulated Efflux. Journal of Biological Chemistry 287(35):29702-29712. PMID: 22722938


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Dopamine Transporter (DAT) thr53 Antibody
Western blot of rat striatal lysate showing specific immunolabeling of the ~55 kDa glycosylated form of the DAT protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

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