Affinity purified from pooled serum. Learn more.

Dopamine Transporter Antibody, Extracellular Loop 2

$109.00$365.00

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Great for use in IHC!

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Human, Non-human primate
Applications:
WB 1:1,000IHC 1:1,000
Species:
Rabbit polyclonal
Gene Name:
SLC6A3
Molecular Reference:
~88 kDa
Cite This Antibody:
PhosphoSolutions Cat# 434-DATEL2, RRID:AB_2492077
Antigen/Purification: ExpandCollapse

The antigen is a peptide from the extracellular loop 2 (EL2) region of human dopamine transporter (DAT), conjugated to keyhole limpet hemocyanin (KLH).

The antibody is prepared from pooled rabbit serum by affinity purification using a SulfoLink® column matrix to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44. PMID: 10936640

Dopamine Transporter Antibody, Extracellular Loop 2
Western blot of human striatal lysate showing specific immunolabeling of the ~88 kDa DAT protein.

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