Affinity purified from pooled serum. Learn more.

Dopamine Transporter Antibody, Extracellular Loop 2

Catalog #: 434-DATEL2 Categories: , , , Datasheet:


  • SizePrice

Great for use in IHC!

Pooled Serum
Affinity Purified from Pooled Serum
Species Reactivity:
Human, Non-human primate
WB 1:1000IHC 1:10
Rabbit polyclonal
Gene Name:
Molecular Weight:
~80 kDa; diffuse band may be observed between ~70-85 kDa due to variable glycosylation
Cite This Antibody:
PhosphoSolutions Cat# 434-DATEL2, RRID:AB_2492077
Antigen/Purification: ExpandCollapse

The antigen is a peptide from the extracellular loop 2 (EL2) region of human dopamine transporter (DAT), conjugated to keyhole limpet hemocyanin (KLH).

The antibody is prepared from pooled rabbit serum by affinity purification using a SulfoLink® column matrix to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).

Synonyms: ExpandCollapse
  • DA transporter antibody
  • DAT 1 antibody
  • DAT antibody
  • DAT1 antibody
  • Dopamine transporter 1 antibody
  • Dopamine transporter antibody
  • PKDYS antibody
  • SC6A3_HUMAN antibody
  • SLC6A3 antibody
  • Sodium dependent dopamine transporter antibody
  • Sodium-dependent dopamine transporter antibody
  • Solute carrier family 6 (neurotransmitter transporter dopamine), member 3 antibody
  • Solute carrier family 6 (neurotransmitter transporter), member 3 antibody
  • Solute carrier family 6 member 3 antibody
  • Variable number tandem repeat (VNTR) antibody


100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44. PMID: 10936640

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Dopamine Transporter Antibody, Extracellular Loop 2
Western blot of human striatal lysate showing specific immunolabeling of the ~88 kDa DAT protein.

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