Affinity purified from pooled serum. Learn more.

Dopamine Transporter Antibody, C-Terminus

Catalog #: 431-DATC Categories: , , , , Datasheet:

$109.00$365.00

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Excellent dopaminergic marker for IHC!

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Non-human primate, Rat
Applications:
WB 1:1000IHC 1:100-1:1000 Don't see your application?
Species:
Rabbit polyclonal
Gene Name:
SLC6A3
Molecular Weight:
~80 kDa; diffuse band may be observed between ~70-85 kDa due to variable glycosylation
Cite This Antibody:
PhosphoSolutions Cat# 431-DATC, RRID:AB_2492076
Antigen/Purification: ExpandCollapse

The antigen is a peptide from the intracellular C-terminus region of human dopamine transporter (DAT), conjugated to keyhole limpet hemocyanin (KLH).

The antibody is prepared from pooled rabbit serum by affinity purification using a SulfoLink® column matrix to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).

Synonyms: ExpandCollapse
  • DA transporter antibody
  • DAT 1 antibody
  • DAT antibody
  • DAT1 antibody
  • Dopamine transporter 1 antibody
  • Dopamine transporter antibody
  • PKDYS antibody
  • SC6A3_HUMAN antibody
  • SLC6A3 antibody
  • Sodium dependent dopamine transporter antibody
  • Sodium-dependent dopamine transporter antibody
  • Solute carrier family 6 (neurotransmitter transporter dopamine), member 3 antibody
  • Solute carrier family 6 (neurotransmitter transporter), member 3 antibody
  • Solute carrier family 6 member 3 antibody
  • Variable number tandem repeat (VNTR) antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Rat
Western Blot: Human


Immunohistochemistry: Rat
PMID Dilution Publication
26321151 1:500 Sarkar, S., et al. 2015 Histopathological and electrophysiological indices of rotenone-evoked dopaminergic toxicity: Neuroprotective effects of acetyl-l-carnitine. Neuroscience Letters, 606, pp.53-59.


Western Blot: Human
PMID Dilution Publication
31529335 not listed Rosas-Hernandez, H., et al. 2019 Stretch-Induced Deformation as a Model to Study Dopaminergic Dysfunction in Traumatic Brain Injury. Neurochemical Research, 44(11), pp.2546-2555.
14535941 1.4ug/ml Haycock, J.W., et al. 2003 Marked disparity between age‐related changes in dopamine and other presynaptic dopaminergic markers in human striatum. Journal of Neurochemistry, 87(3), pp.574-585.
11282256 not listed Kish, S.J., et al. 2001 Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology, 24(5), p.561.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Dopamine Transporter Antibody, C-Terminus
Western blot of human striatal lysate showing specific immunolabeling of the ~88 kDa DAT protein.

Click here to view our Western blotting and lysate preparation protocols.

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