Affinity purified from pooled serum. Learn more.

Dopamine Transporter Antibody, C-Terminus

$109.00$365.00

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Excellent marker for IHC!

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Mouse, Human, Non-human primate
Applications:
WB 1:1,000IHC 1:1,000
Species:
Rabbit polyclonal
Gene Name:
SLC6A3
Molecular Reference:
~88 kDa
Cite This Antibody:
PhosphoSolutions Cat# 431-DATC, RRID:AB_2492076
Antigen/Purification: ExpandCollapse

The antigen is a peptide from the intracellular C-terminus region of human dopamine transporter (DAT), conjugated to keyhole limpet hemocyanin (KLH).

The antibody is prepared from pooled rabbit serum by affinity purification using a SulfoLink® column matrix to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Sarkar, S., Gough, B., Raymick, J., Beaudoin, M. A., Ali, S. F., Virmani, A., & Binienda, Z. K. (2015). Histopathological and electrophysiological indices of rotenone-evoked dopaminergic toxicity: Neuroprotective effects of acetyl-l-carnitine. Neuroscience letters, 606, 53-59. PMID: 26321151

Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567. PMID: 11282256

Dopamine Transporter Antibody, C-Terminus
Western blot of human striatal lysate showing specific immunolabeling of the ~88 kDa DAT protein.

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