Affinity purified from pooled serum. Learn more.

Dopamine β-Hydroxylase Antibody, C-Terminus

Catalog #: 420-DBHC Categories: , , , , Datasheet:

$109.00$365.00

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Sheep polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Non-human primate
Applications:
WB 1:1000 Don't see your application?
Species:
Sheep
Gene Name:
DBH
Molecular Weight:
~75 kDa
Cite This Antibody:
PhosphoSolutions Cat# 420-DBHC, RRID:AB_2492074
Antigen/Purification: ExpandCollapse

The antigen is a peptide from the C-terminal region of human dopamine β-hydroxylase (DBH), conjugated to keyhole limpet hemocyanin (KLH).

The antibody is prepared from pooled sheep serum by affinity purification using a Sulfo-Link® column matrix to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

DBH catalyzes the conversion of dopamine to norepinephrine and serves as a marker of noradrenergic cells. DBH antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). The expression of DBH is also elevated during stress (Sabban and Kvetnansky, 2001).

Synonyms: ExpandCollapse
  • dbh antibody
  • DBM antibody
  • Dopamine beta hydroxylase antibody
  • Dopamine beta monooxygenase antibody
  • Dopamine beta-hydroxylase (dopamine beta-monooxygenase) antibody
  • Dopamine beta-monooxygenase antibody
  • DOPO_HUMAN antibody
  • OTTHUMP00000022501 antibody
  • Soluble dopamine beta-hydroxylase antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Kish, S.J., Kalasinsky, K.S., Derkach, P., Schmunk, G.A., Guttman, M., Ang, L., Adams, V., Furukawa, Y. and Haycock, J.W., 2001. Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology, 24(5), p.561. PMID: 11282256

Sabban, E.L. and Kvetňanský, R., 2001. Stress-triggered activation of gene expression in catecholaminergic systems: dynamics of transcriptional events. Trends in Neurosciences, 24(2), pp.91-98. PMID: 11164939

Zhu, M.Y., Klimek, V., Dilley, G.E., Haycock, J.W., Stockmeier, C., Overholser, J.C., Meltzer, H.Y. and Ordway, G.A., 1999. Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biological Psychiatry, 46(9), pp.1275-1286. PMID: 10560033

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Dopamine β-Hydroxylase Antibody, C-Terminus
Western blot of human adrenal medulla lysate showing specific immunolabeling of the ~75 kDa DBH protein.

Click here to view our Western blotting and lysate preparation protocols.

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