Affinity purified from pooled serum. Learn more.

Dopamine β-Hydroxylase Antibody, C-Terminus

$109.00$365.00

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Sheep polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Mouse, Human, Non-human primate
Applications:
WB 1:1,000
Species:
Sheep
Gene Name:
DBH
Molecular Reference:
~75 kDa
Cite This Antibody:
PhosphoSolutions Cat# 420-DBHC, RRID:AB_2492074
Antigen/Purification: ExpandCollapse

The antigen is a peptide from the C-terminal region of human dopamine β-hydroxylase (DBH), conjugated to keyhole limpet hemocyanin (KLH).

The antibody is prepared from pooled sheep serum by affinity purification using a Sulfo-Link® column matrix to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

DBH catalyzes the conversion of dopamine to norepinephrine and serves as a marker of noradrenergic cells. DBH antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). The expression of DBH is also elevated during stress (Sabban and Kvetnansky, 2001).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44. PMID: 10936640

Dopamine β-Hydroxylase Antibody, C-Terminus
Western blot of human adrenal medulla lysate showing specific immunolabeling of the ~75 kDa DBH protein.

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