Affinity purified from pooled serum. Learn more.

DiGeorge Syndrome Critical Region 8 DGCR8 (Ser377) Antibody

Catalog #: p1035-377 Categories: , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
100% Homology:
Mouse, Human
Applications:
WB 1:1,000
Species:
Rabbit
Gene Name:
DGCR8
Molecular Reference:
~120 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1035-377, RRID:AB_2492071
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser377 of human DGCR8.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

The Drosha-DGCR8 microprocessor complex is required for microRNA (miRNA) biogenesis. DGCR8 (DiGeorge Syndrome Critical Region 8) recognizes the RNA substrate, whereas Drosha functions as the endonuclease. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, interacts with the pri-miRNA and functions as the molecular anchor that measures the distance from the ds-RNA-ssRNA junction and directs Drosha cleavage 11bp away (Han J et al, 2006). The efficiency of Drosha cleavage increases in the presence of heme and promotes the formation of highly ordered DGCR8 structures upon binding to RNA (Faller et al, 2010).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Han J, Lee Y, Yeom KH, Nam JW Heo I, Rhee JK, Sohn SY, Cho Y, Zhang BT, Kim VN (2006). Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex. Cell Jun 2; 125(5): 887-901.

Faller M, Toso D, Matsunaga M, Atanasov I, Senturia R, Chen Y, Zhou Zh, Guo F (2010). DGCR8 recognizes primary transcripts of microRNAs through highly cooperative binding and formation of higher-order structures. RNA 2010 Aug;16(8):1570-83.

DiGeorge Syndrome Critical Region 8 DGCR8 ser377 Antibody
Western blot of mouse nuclei lysate showing specific immunolabeling of the ~120 kDa DGCR8 protein phosphorylated at in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

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