Affinity purified from pooled serum. Learn more.

DiGeorge Syndrome Critical Region 8 DGCR8 (Ser377) Antibody

We are the original manufacturer of our DGCR8 (Ser377) rabbit polyclonal phospho specific antibody, affinity purified from pooled serum. Optimized in WB.

Catalog #: p1035-377 Categories: , , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse
Applications:
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
DGCR8
Molecular Weight:
~120 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1035-377, RRID:AB_2492071
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser377 of human DGCR8.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

The Drosha-DGCR8 microprocessor complex is required for microRNA (miRNA) biogenesis. DGCR8 (DiGeorge Syndrome Critical Region 8) recognizes the RNA substrate, whereas Drosha functions as the endonuclease. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, interacts with the pri-miRNA and functions as the molecular anchor that measures the distance from the ds-RNA-ssRNA junction and directs Drosha cleavage 11bp away (Han, J., et al, 2006). The efficiency of Drosha cleavage increases in the presence of heme and promotes the formation of highly ordered DGCR8 structures upon binding to RNA (Faller, M., et al, 2010).

Synonyms: ExpandCollapse
  • DGCRK6 antibody
  • C22orf12 antibody
  • D16H22S788E antibody
  • D16Wis2 antibody
  • DGCR 8 antibody
  • Dgcr8 antibody
  • DGCR8 microprocessor complex subunit antibody
  • DGCR8_HUMAN antibody
  • DGCRK 6 antibody
  • DiGeorge syndrome critical region 8 antibody
  • DiGeorge syndrome critical region gene 8 antibody
  • Gy1 antibody
  • Microprocessor complex subunit DGCR8 antibody
  • pasha antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Han, J., Lee, Y., Yeom, K.H., Nam, J.W., Heo, I., Rhee, J.K., Sohn, S.Y., Cho, Y., Zhang, B.T. and Kim, V.N., 2006. Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex. Cell, 125(5), pp.887-901. PMID: 16751099

Faller, M., Toso, D., Matsunaga, M., Atanasov, I., Senturia, R., Chen, Y., Zhou, Z.H. and Guo, F., 2010. DGCR8 recognizes primary transcripts of microRNAs through highly cooperative binding and formation of higher-order structures. RNA, 16(8), pp.1570-1583. PMID: 20558544

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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