Rabbit polyclonal antibodyPooled Serum
- Affinity Purified from Pooled Serum
- 100% Homology:
- Mouse, Human
- WB 1:1,000
- Gene Name:
- Molecular Reference:
- ~120 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p1035-377, RRID:AB_2492071
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser377 of human DGCR8.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Biological Significance: ExpandCollapse
The Drosha-DGCR8 microprocessor complex is required for microRNA (miRNA) biogenesis. DGCR8 (DiGeorge Syndrome Critical Region 8) recognizes the RNA substrate, whereas Drosha functions as the endonuclease. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, interacts with the pri-miRNA and functions as the molecular anchor that measures the distance from the ds-RNA-ssRNA junction and directs Drosha cleavage 11bp away (Han J et al, 2006). The efficiency of Drosha cleavage increases in the presence of heme and promotes the formation of highly ordered DGCR8 structures upon binding to RNA (Faller et al, 2010).