DARPP 32 (Thr75) Antibody

Catalog #: p1025-75 Categories: , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Formulation:
Affinity purified
Species Tested:
Mouse, Rat
Expected Reactivity:
Human, Bovine, Chicken, Non-human primate, Canine
Applications:
WB 1:1000 Don't see your application?
Species:
Rabbit
Gene Name:
PPP1R1B
Molecular Weight:
~32 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1025-75, RRID:AB_2492069
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr75 of rat DARPP-32.

The antibody is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

DARPP-32 is a dopamine (DA) and cAMP-regulated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).

Synonyms: ExpandCollapse
  • DARPP32 antibody
  • DARPP-32 antibody
  • Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
  • Dopamine and cAMP regulated neuronal phosphoprotein antibody
  • Dopamine and cAMP regulated phosphoprotein antibody
  • Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody
  • Dopamine and cAMP regulated phosphoprotein DARPP32 antibody
  • Dopamine- and cAMP-regulated neuronal phosphoprotein antibody
  • FLJ20940 antibody
  • IPPD antibody
  • Neuronal phosphoprotein DARPP 32 antibody
  • PPP1R1B antibody
  • PPR1B_HUMAN antibody
  • Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody
  • Protein phosphatase 1 regulatory inhibitor subunit 1B antibody
  • Protein phosphatase 1 regulatory subunit 1B antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Mouse


Western Blot: Mouse
PMID Dilution Publication
21946266 not listed Errico, F., et al. 2011 Higher free D-aspartate and N-methyl-D-aspartate levels prevent striatal depotentiation and anticipate L-DOPA-induced dyskinesia. Experimental neurology, 232(2), pp.240-250.
20720111 1:1000 Napolitano, F., et al. 2010 Role of aberrant striatal dopamine D1 receptor/cAMP/protein kinase A/DARPP32 signaling in the paradoxical calming effect of amphetamine. Journal of Neuroscience, 30(33), pp.11043-11056.
18234897 1:1000 Niculescu, M., et al. 2008 Trk: a neuromodulator of age-specific behavioral and neurochemical responses to cocaine in mice. Journal of Neuroscience, 28(5), pp.1198-1207.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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DARPP 32 thr75 Antibody
Western blot of rat striatal lysate showing specific immunolabeling of the ~32 kDa DARPP-32 phosphorylated at Thr75 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Click here to view our Western blotting and lysate preparation protocols.

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