Affinity purified from pooled serum. Learn more.

DARPP 32 (Ser137) Antibody

Catalog #: p1025-137 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse, Human, Bovine, Chicken, Non-human primate, Canine
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
PPP1R1B
Molecular Reference:
~32 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1025-137, RRID:AB_2492070
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser137 of rat DARPP-32.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

DARPP-32 is a dopamine (DA) and cAMP-regulated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002). Serine 137 is phosphorylated by casein kinase 1 and phosphorylation of this site is increased following acute administration of Prozac® (Svenningsson et al., 2002).

Synonyms: ExpandCollapse
  • DARPP32 antibody
  • DARPP-32 antibody
  • Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
  • Dopamine and cAMP regulated neuronal phosphoprotein antibody
  • Dopamine and cAMP regulated phosphoprotein antibody
  • Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody
  • Dopamine and cAMP regulated phosphoprotein DARPP32 antibody
  • Dopamine- and cAMP-regulated neuronal phosphoprotein antibody
  • FLJ20940 antibody
  • IPPD antibody
  • Neuronal phosphoprotein DARPP 32 antibody
  • PPP1R1B antibody
  • PPR1B_HUMAN antibody
  • Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody
  • Protein phosphatase 1 regulatory inhibitor subunit 1B antibody
  • Protein phosphatase 1 regulatory subunit 1B antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Bellini, S., Fleming, K.E., De, M., McCauley, J.P., Petroccione, M.A., D’Brant, L.Y., Tkachenko, A., Kwon, S., Jones, L.A. and Scimemi, A., 2018. Neuronal glutamate transporters control dopaminergic signaling and compulsive behaviors. Journal of Neuroscience, 38(4), pp.937-961. PMID: 29229708

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DARPP 32 ser137 Antibody
Western blot of rat striatal lysate showing specific immunolabeling of the ~32 kDa DARPP-32 phosphorylated at Ser137 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

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