Affinity purified from pooled serum. Learn more.

DARPP 32 Antibody

Catalog #: 374-DARPP Categories: , , Datasheet:

$109.00$365.00

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Excellent marker for striatal medium spiny neurons!

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse, Human, Xenopus, Bovine, Chicken, Non-human primate, Canine
Applications:
WB 1:1000IHC 1:250
Species:
Rabbit polyclonal
Gene Name:
PPP1R1B
Molecular Reference:
~32 kDa
Cite This Antibody:
PhosphoSolutions Cat# 374-DARPP, RRID:AB_2492067
Antigen/Purification: ExpandCollapse

The antigen is a peptide corresponding to amino acid residues from the N-terminal region of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH).

The antibody is prepared from pooled rabbit serum by affinity purification via chromatography on an affinity column prepared with the N-terminal peptide used as antigen.

Biological Significance: ExpandCollapse

DARPP-32 is a dopamine (DA) and cAMP-regulated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).

Synonyms: ExpandCollapse
  • DARPP32 antibody
  • DARPP-32 antibody
  • Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
  • Dopamine and cAMP regulated neuronal phosphoprotein antibody
  • Dopamine and cAMP regulated phosphoprotein antibody
  • Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody
  • Dopamine and cAMP regulated phosphoprotein DARPP32 antibody
  • Dopamine- and cAMP-regulated neuronal phosphoprotein antibody
  • FLJ20940 antibody
  • IPPD antibody
  • Neuronal phosphoprotein DARPP 32 antibody
  • PPP1R1B antibody
  • PPR1B_HUMAN antibody
  • Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody
  • Protein phosphatase 1 regulatory inhibitor subunit 1B antibody
  • Protein phosphatase 1 regulatory subunit 1B antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Bonito-Oliva, A., DuPont, C., Madjid, N., Ögren, S. O., & Fisone, G. (2015). Involvement of the striatal medium spiny neurons of the direct pathway in the motor stimulant effects of phencyclidine. The international journal of neuropsychopharmacology/(CINP). PMID: 26657176

Michelle Niculescu, Shane A. Perrine, Jonathan S. Miller, Michelle E. Ehrlich, and Ellen M. Unterwald (2008) Trk: A Neuromodulator of Age-Specific Behavioral and Neurochemical Responses to Cocaine in Mice. J. Neurosci., Jan 2008; 28: 1198 – 1207. PMID: 18234897

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DARPP 32 Antibody
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~32 kDa DARPP protein.
DARPP 32 Antibody
Immunochemical staining of cultured CD1 mouse striatal cells showing specific labeling of DARPP-32 in green and Tuj1 in red. Cells and photo courtesy of QBM CellScience.

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