Affinity purified from pooled serum. Learn more.

CtIP (Ser327) Antibody

Catalog #: p1012-327 Categories: , Datasheet:

$119.00$380.00

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Rabbit Polyclonal Antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human
Expected Reactivity:
Non-human primate
Applications:
WB 1:1000 Don't see your application?
Species:
Rabbit Polyclonal
Molecular Weight:
~ 100 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1012-327, RRID:AB_2651148
Antigen/Purification: ExpandCollapse

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser327 of human CtIP.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Biological Significance: ExpandCollapse

CtIP, C-terminal binding protein-interacting protein, is a DNA endonuclease activated by double stranded breaks (DSBs).  DSB repairs can be performed by either one of two mechanisms; non-homologous end joining (NHEJ) or homologous recombination (HR).  NHEJ is the predominant DSB repair pathway throughout the entire cell cycle, most importantly in the G1 phase (Rothkamm et al, 2003); while HR is important for repairing DSBs in S and G2 phases (Beucher et al, 2009). CtIP controls DSB resection; an event that only occurs in HR during G2-phase. Phosphorylation of Thr847 dictates the resection efficiency (Huertas et al, 2008). Furthermore, it has been found that DSBs undergo resection and repair in G1-phase cells via a process requiring Plk3 phosphorylation of CtIP at Ser327 and Thr847 (Barton et al, 2014).  Several additional phosphorylation sites within CtIP have been identified, but their significance in the repair of DNA have yet to be determined.

Synonyms: ExpandCollapse
  • COM1 antibody
  • COM1_HUMAN antibody
  • CtBP interacting protein antibody
  • CtBP-interacting protein antibody
  • CtIP antibody
  • DNA endonuclease RBBP8 antibody
  • JWDS antibody
  • RB binding protein 8 endonuclease antibody
  • RBBP-8 antibody
  • RBBP8 antibody
  • Retinoblastoma-binding protein 8 antibody
  • Retinoblastoma-interacting protein and myosin-like antibody
  • Rim antibody
  • SAE2 antibody
  • SCKL2 antibody
  • Sporulation in the absence of SPO11 protein 2 homolog antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Human


Western Blot: Human
PMID Dilution Publication
25267294 not listed Barton, O., et al. 2014 Polo-like kinase 3 regulates CtIP during DNA double-strand break repair in G1. J Cell Biol, 206(7), pp.877-894.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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