CRISPR Cas9 Antibody

Catalog #: 304-Cas9 Category: Datasheet:

$109.00$365.00

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Mouse Monoclonal Antibody

Formulation:
Protein G purified culture supernatant
Species Tested:
Specific for the Staphylococcus aureus Cas9 protein
Applications:
WB 1:1000IHC 1:1000-1:5000ICC 1:500-1:5000
Species:
Mouse Monoclonal
Molecular Reference:
~53 kDa
Cite This Antibody:
PhosphoSolutions Cat# 304-CAS9, RRID:AB_2715491
Antigen/Purification: ExpandCollapse

Recombinant protein from C-terminal region of Cas9 from Staphylococcus aureus.

The antibody is Protein G purified from culture supernatant.

Biological Significance: ExpandCollapse

CRISPR/Cas9 is a genome editing tool that makes possible the permanent alteration of genes by directing specifically designed RNA sequences complexed with the nuclease Cas9 to cleave DNA at any desired location allowing the deletion or addition of genes.  Antibodies against Cas9 are very useful in the direct detection of the CRISPR/Cas9 complex. This antibody detects Cas9 from Staphylococcus aureus transfected into cells and shows no reactivity toward the Streptococcus pyogenes Cas9 protein (Ran et al., 2015).

Synonyms: ExpandCollapse
  • Cas9 antibody
  • CRISPR-associated endonuclease Cas9/Csn1 antibody
  • CRISPR-Cas9/Csn1 antibody
  • csn1 antibody
  • SpyCas9 antibody

Storage

100 µl in PBS + 50% glycerol and 5 mM NaN3. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunocytochemistry

HEK293 cells transfected with a fusion construct of GFP with the C-terminal region of S. aureas Cas9 protein.

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General Reference

Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. (2015) In vivo genome editing using Staphylococcus aureus Cas9. Nature 520:186-91.

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Western blot of CRISPR Cas9 Antibody
Western blot of GFP-Cas9 transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~53 kDa GFP-Cas9 fusion protein. Labeling is absent in the untransfected lysate (-).

Click here to view our Western blotting and lysate preparation protocols.

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