CRISPR Cas9 Antibody

Catalog #: 304-Cas9 Category: Datasheet:

$109.00$365.00

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Mouse Monoclonal Antibody

Formulation:
Protein G purified culture supernatant
Specificity:
Specific for the Staphylococcus aureus Cas9 protein
Applications:
WB 1:1000IF 1:500
Species:
Mouse Monoclonal
Cite This Antibody:
PhosphoSolutions Cat# 304-CAS9, RRID:AB_2715491
Antigen/Purification: ExpandCollapse

Recombinant protein from C-terminal region of Cas9 from Staphylococcus aureus.

The antibody is Protein G purified from culture supernatant.

Biological Significance: ExpandCollapse

CRISPR/Cas9 is a genome editing tool that makes possible the permanent alteration of genes by directing specifically designed RNA sequences complexed with the nuclease Cas9 to cleave DNA at any desired location allowing the deletion or addition of genes.  Antibodies against Cas9 are very useful in the direct detection of the CRISPR/Cas9 complex. This antibody detects Cas9 from Staphylococcus aureus transfected into cells and shows no reactivity toward the Streptococcus pyogenes Cas9 protein (Ran et al., 2015).

Storage

100 µl in PBS + 50% glycerol and 5 mM NaN3. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General Reference

Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. (2015) In vivo genome editing using Staphylococcus aureus Cas9. Nature 520:186-91.

Western blot of CRISPR Cas9 Antibody
Western blot of GFP-Cas9 transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~53 kDa GFP-Cas9 fusion protein. Labeling is absent in the untransfected lysate (-).
Immunofluorescence of HEK293 cells transfected with Cas9 and labeled with Cas9 antibody.

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