CRISPR Cas9 Antibody

Catalog #: 304-Cas9 Categories: , Datasheet:

$119.00$380.00

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Mouse Monoclonal Antibody

Formulation:
Protein G purified culture supernatant
Species Tested:
Specific for the Staphylococcus aureus Cas9 protein
Applications:
WB 1:1000IHC 1:1000-1:5000ICC 1:500-1:5000 Don't see your application?
Species:
Mouse Monoclonal
Molecular Weight:
~53 kDa
Cite This Antibody:
PhosphoSolutions Cat# 304-CAS9, RRID:AB_2715491
Antigen/Purification: ExpandCollapse

Recombinant protein from C-terminal region of Cas9 from Staphylococcus aureus.

The antibody is Protein G purified from culture supernatant.

Biological Significance: ExpandCollapse

CRISPR/Cas9 is a genome editing tool that makes possible the permanent alteration of genes by directing specifically designed RNA sequences complexed with the nuclease Cas9 to cleave DNA at any desired location allowing the deletion or addition of genes.  Antibodies against Cas9 are very useful in the direct detection of the CRISPR/Cas9 complex. This antibody detects Cas9 from Staphylococcus aureus transfected into cells and shows no reactivity toward the Streptococcus pyogenes Cas9 protein (Ran et al., 2015).

Synonyms: ExpandCollapse
  • Cas9 antibody
  • CRISPR-associated endonuclease Cas9/Csn1 antibody
  • CRISPR-Cas9/Csn1 antibody
  • csn1 antibody
  • SpyCas9 antibody

Storage

100 µl in PBS + 50% glycerol and 5 mM NaN3. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunocytochemistry

HEK293 cells transfected with S. aureas Cas9 protein.

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Ran, F.A., Cong, L., Yan, W.X., Scott, D.A., Gootenberg, J.S., Kriz, A.J., Zetsche, B., Shalem, O., Wu, X., Makarova, K.S. and Koonin, E.V., 2015. In vivo genome editing using Staphylococcus aureus Cas9. Nature, 520(7546), p.186. PMID: 25830891

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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