Affinity purified from pooled serum. Learn more.

Creatine Kinase, mitochondrial (CK1Mt) (Tyr153) Antibody

Catalog #: p145-153 Categories: , Datasheet:

$109.00$365.00

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Rabbit Polyclonal Antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Bovine, Canine, Guinea pig, Hamster, Human, Mouse, Non-human primate, Rabbit, Rat, Sheep
Applications:
WB 1:1,000
Species:
Rabbit Polyclonal
Molecular Reference:
~46 kDa
Cite This Antibody:
PhosphoSolutions Cat# p145-153, RRID:AB_2632396
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho Tyr153 of human CK1Mt.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Biological Significance: ExpandCollapse

Creatine kinase (CK1) plays an important role transferring phosphate groups from phosphocreatine to ADP in the cytosol of tissues having high, fluctuating energy demands like skeletal muscle, heart, and brain.  Mitochondrial CK1, CK1Mt, has two isoforms, sarcomeric CK1Mt and ubiquitous CK1Mt.  CK1Mt is localized between the MIM (mitochondrial inner membrane) and MOM (mitochondrial outer membrane) and bound to the cardiolipin-rich inner leaflet (Muller et al., 1985) and along the cristae membranes (Wegmann et al., 1991).  CK1Mt exists in two forms; a homo dimer and an octamer consisting of four homodimers.  The CK1Mt octamer forms permanent contact sites and maintains a complex structure including porin and ANT (adenine nucleotide translocase) within MIM and MOM (Speer et al, 2005). There have been several phospho-serine, threonine, and tyrosine sites identified within CK1Mt, the role of each one has yet to be determined.

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Müller, M., Moser, R., Cheneval, D., Carafoli, E. (1985) Cardiolipin is the Membrane Receptor for Mitochondrial Creatine Phosphokinase. J. Biol. Chem. 260, 3839-3843.

Wegmann, G., Huber, R., Zanolla, E., Eppenberger, H. M., Wallimann, T. (1991) Differential Expression and Localization of Brain-Type and Mitochondrial Creatine Kinase Isoenzymes During Development of the Chicken Retina: Mi-CK as a Marker for Differentiation of Photoreceptor Cells. Differentiation 46, 77-87.

Speer O, Bäck N, Buerklen T, Brdiczka D, Koretsky A, Walliman T, Eriksson O (2005) Octameric Mitochondrial Creatine Kinase Induces and Stabilizes Contact Sites Between the Inner and Outer Membrane. Biochem. J. 385, 445-450.

CK1Mt tyr153 antibody
Western blot of mouse mitochondria from whole brain lysate showing specific immunolabeling of the ~46 kDa CK1Mt protein phosphorylated at Tyr153 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is greatly decreased by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

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