Affinity purified from pooled serum. Learn more.

COXIV (Ser58) Antibody

We are the original manufacturer of our COXIV (Ser58) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. Optimized in WB.

Catalog #: p1008-58 Categories: , , , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Applications:
WB 1:1000 Don't see your application?
Host Species:
Rabbit
Gene Name:
COX4i1
Molecular Weight:
~17 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1008-58, RRID:AB_2492065
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser58 of mouse COXIV protein.

The antibody is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

COX, also known as cytochrome c oxidase, has 12 subunits that make up the transmembrane mitochondrial protein. Subunit IV has two isoforms; COXIV-1 and COXIV-2 (Huttermann et al., 2001). COXIV-1 is expressed ubiquitously while COXIV-2 is highly expressed in adult lung and low levels in brain and heart (Huttermann et al., 2001). Phosphorylation of amino acid residue Ser58 of the COXIV-1 protein is a PKA-dependent regulation of COX and plays an important role in metabolism and CREB cycle activation (AcinPerez et al., 2011).

Synonyms: ExpandCollapse
  • AL024441 antibody
  • COX 4 antibody
  • COX IV 1 antibody
  • COX IV antibody
  • COX IV-1 antibody
  • Cox4 antibody
  • COX41_HUMAN antibody
  • Cox4a antibody
  • COX4B antibody
  • COX4I1 antibody
  • COX4I2 antibody
  • COX4L2 antibody
  • Cytochrome c oxidase polypeptide IV antibody
  • Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody
  • Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody
  • Cytochrome C Oxidase subunit IV antibody
  • Cytochrome c oxidase subunit IV isoform 1 antibody
  • Cytochrome c oxidase subunit IV isoform 2 (lung) antibody
  • Cytochrome c oxydase subunit 4 antibody
  • 2 antibody
  • MGC105470 antibody
  • MGC72016 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Mouse | Rat


Western Blot: Mouse
PMID Dilution Publication
27973394 1:500 Neviere, R., et al. 2016 Abnormal mitochondrial cAMP/PKA signaling is involved in sepsis-induced mitochondrial and myocardial dysfunction. International journal of molecular sciences, 17(12), p.2075.


Western Blot: Rat
PMID Dilution Publication
21641552 not listed Acin-Perez, R., et al. 2011 Protein Phosphorylation and Prevention of Cytochrome Oxidase Inhibition by ATP: Coupled Mechanisms of Energy Metabolism Regulation. Cell Metabolism, 13, 712-719.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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