Affinity purified from pooled serum. Learn more.

COXIV (Ser58) Antibody

Catalog #: p1008-58 Categories: , , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
COX4i1
Molecular Weight:
~17 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1008-58, RRID:AB_2492065
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser58 of mouse COXIV protein.

The antibody is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

COX, also known as cytochrome c oxidase, has 12 subunits that make up the transmembrane mitochondrial protein. Subunit IV has two isoforms; COXIV-1 and COXIV-2 (Huttermann et al., 2001). COXIV-1 is expressed ubiquitously while COXIV-2 is highly expressed in adult lung and low levels in brain and heart (Huttermann et al., 2001). Phosphorylation of amino acid residue Ser58 of the COXIV-1 protein is a PKA-dependent regulation of COX and plays an important role in metabolism and CREB cycle activation (AcinPerez et al., 2011).

Synonyms: ExpandCollapse
  • AL024441 antibody
  • COX 4 antibody
  • COX IV 1 antibody
  • COX IV antibody
  • COX IV-1 antibody
  • Cox4 antibody
  • COX41_HUMAN antibody
  • Cox4a antibody
  • COX4B antibody
  • COX4I1 antibody
  • COX4I2 antibody
  • COX4L2 antibody
  • Cytochrome c oxidase polypeptide IV antibody
  • Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody
  • Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody
  • Cytochrome C Oxidase subunit IV antibody
  • Cytochrome c oxidase subunit IV isoform 1 antibody
  • Cytochrome c oxidase subunit IV isoform 2 (lung) antibody
  • Cytochrome c oxydase subunit 4 antibody
  • 2 antibody
  • MGC105470 antibody
  • MGC72016 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Neviere, R., Delguste, F., Durand, A., Inamo, J., Boulanger, E. and Preau, S., 2016. Abnormal mitochondrial cAMP/PKA signaling is involved in sepsis-induced mitochondrial and myocardial dysfunction. International journal of molecular sciences, 17(12), p.2075. PMID: 27973394

Acin-Perez R, Gatti D, Bai Y, Manfredi D (2011) Protein Phosphorylation and Prevention of Cytochrome Oxidase Inhibition by ATP: Coupled Mechanisms of Energy Metabolism Regulation. Cell Metabolism 13, 712-719. PMID: 21641552

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Western blot of rat mitochondrial lysate showing specific immunolabeling of the ~17 kDa COXIV phosphorylated at Ser58 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

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